Hydrocortisol

Dp was purchased from Mansite Bio-technology Co (Chengdu, China); DMEM/F12 medium and FBS were purchased from HyClone (Beijing, China); Trizol reagent, horse serum, gentamicin, insulin, Lipofectamine 2000, Opti-Mem were purchased from Invitrogen (Carlsbad, CA, USA); Epidermal growth factor (EFG) was purchased from PeproTech Inc (Rocky Hill, USA); PathScan Phospho-Akt ELISA assay kit and all antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), benzo[a]pyrene (B[a]P), cholera enterotoxin, hydrocortisol, dimethylsulfoxide (DMSO), phosphate buffered saline (PBS) and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). All cell lines were purchased from Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China)

Resveratrol (R5010), cholera enterotoxin, hydrocortisol and CQ (chloroquine) were purchased from Sigma-Aldrich (St. Louis, MO, USA); DMEM/F12, DMEM, Ham's F12 medium, FBS (fetal bovine serum) and BSA (bovine serum albumin) were purchased from HyClone (Beijing, China); Trizol reagent, horse serum, medium 199, antibioticantimycotic, gentamicin, insulin, Lipofectamine 2000, Opti-Mem were purchased from Invitrogen (Carlsbad, CA, USA); EFG and bFGF (Basic fibroblast growth factor) were purchased from PeproTech Inc (Rocky Hill, USA); B-27 was purchased from Gibco (Gaithersburg, MD, USA); The Cell Counting Kit (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). Aldefluor assay kit and collagenase/hyaluronidase were purchased from StemCell Technologies (Vancouver, Canada); Antibodies to β-catenin was purchased from Bioworld Technology (Minneapolis, MN, USA); Antibodies to cyclin D1, Beclin1 and Atg 7 were purchased from Santa Cruz (CA, USA); Antibodies to LC3 were purchased from Cell Signaling Technology (Danvers, MA, USA). The GFP- LC3-II plasmids were kindly provided by Dr. Tamotsu Yoshimori (National Institute for Basic Biology, Okazaki, Japan). The plasmid of pcDNA3-S33Y β-catenin (Plasmid 19286) was provided by Addgene (MA, USA).

The JIMT-1 human breast carcinoma cell line (ACC589) was purchased from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). The normal-like breast epithelial MCF-10A cell line (CRL-10317), the cancer cell lines MCF-7 (HTB-22) and HCC1937 (CRL-2336) were purchased from American Type Culture Collection (Manassas, VA, USA). The cells were tested negative for mycoplasma (Eurofins, Konstanz, Germany).
The JIMT-1 cells were routinely cultured at 37 °C in a humidified incubator with 5% CO₂ in air. The cells were cultured in DMEM/Ham’s F-12 medium supplemented with 10% fetal bovine serum (FBS), glutamine (2 mM), non-essential amino acids (1 mM), insulin (10 μg/ml), penicillin (100 U/ml), and streptomycin (100 μg/ml).
MCF-10A, MCF-7, and HCC1937 cell lines were cultured in RPMI 1640 medium (VWR) supplemented with 10% heat-inactivated FBS (VWR, Lund, Sweden), glutamine (2 mM), 1 mM non-essential amino acids (VWR), 10 μg/ml insulin (Sigma-Aldrich, Stockholm, Sweden), and 100 U/ml penicillin/100 μg/ml streptomycin (VWR). The MCF-10A cells were also supplemented with 20 ng/ml epidermal growth factor (Sigma-Aldrich), 50 ng/ml cholera toxin (Sigma-Aldrich), and 250 ng/ml hydrocortisol (Sigma-Aldrich). Finally, the HCC1937 medium was supplemented with 20 ng/ml epidermal growth factor (Sigma-Aldrich) besides the mentioned supplements.