The effect of exosomes on C2C12 myoblast proliferative capacities was monitored with the xCELLigence live cell analysis System (Life Science Roche, Meylan, France), which offers dynamic live cell monitoring [22 (link)]. One day post-plating, cells were grown in serum-free DMEM and 4.5 g/l glucose supplemented with exosomes, and monitored every 15 min for 24 h [15 (link)]. At the end of the experiment, cells were trypsinised, counted and their sizes were determined by using the Scepter 2.0 handheld automated cell counter from Millipore (Guyancourt, France).
Scepter 2.0 handheld
Manufactured by Merck Group
Sourced in France, United States
The Scepter 2.0 handheld is a laboratory instrument designed for cell counting and viability analysis. It provides rapid and precise measurements of cell populations in small sample volumes.
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Lab products found in correlation
3 protocols using scepter 2.0 handheld
1
Exosomes' Effect on Myoblast Proliferation
2
Doxycycline-Inducible Luciferase Assay
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HEK293 Tet-On 3G cells (Clontech) expressing the pTETOnLux plasmid were washed once with PBS, harvested, and counted using a Scepter 2.0 handheld automated cell counter (Millipore). Approximately 1×106 cells/well were plated in 1 ml medium volumes in a black 24-well plate. Doxycycline was added at final concentrations of 0 (control), 5, 10, 50 or 500 ng/ml in triplicate wells. Bioluminescence measurements were obtained immediately after doxycycline induction using an IVIS Lumina imaging system with a 10 min integration time every 30 min for 16 h.
3
Cell Enumeration Techniques for UC Tissue
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Enumeration of cells from freshly digested UC tissue and of thawed cells from digested tissue was performed by hemocytometer. Enumeration of cells collected at the end of explant culture, of expanded c-UCMSCs and d-UCMSCs at the end of each passage and of daily samples during growth curve analysis studies was performed using a handheld cytometer (Scepter 2.0 handheld automated cell counter; Millipore Corporation, Billerica, MA, USA).
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