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Anti cd105 pe clone sn6

Manufactured by Thermo Fisher Scientific

Anti-CD105-PE (clone SN6) is a fluorescently labeled monoclonal antibody that binds to the CD105 (endoglin) antigen. CD105 is a cell surface marker expressed on endothelial cells and is involved in angiogenesis. This product can be used for flow cytometry applications to detect and quantify CD105-expressing cells.

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2 protocols using anti cd105 pe clone sn6

1

Multiparametric Characterization of Cellular Phenotypes

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Antibodies used for staining included: anti-pan-cytokeratin (clone C11, 1:500) and vimentin (clone D21H3, 1:200) (Cell Signaling), anti- α-smooth muscle actin (α-SMA) (clone 1A4, 1:200) (Dako), anti-Ki-67 (rabbit polyclonal, ab15580, 1:300) (Abcam), anti-CD105-PE (clone SN6, 1:100) (eBioscience), anti-mitochondria (clone 113-1, 1:100) and anti-fibroblast specific protein-1 (FSP-1; rabbit polyclonal, 07-2274, 1:200) (Millipore). IL-6 ELISA kit was purchased from BD Biosciences. CCL5 DuoSet ELISA kit and mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit were from R&D Systems. Alexa Fluor® fluorescent dye-conjugated secondary antibodies were from Life Technologies for immunofluorescent staining.
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2

Multiparametric Characterization of Cellular Phenotypes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for staining included: anti-pan-cytokeratin (clone C11, 1:500) and vimentin (clone D21H3, 1:200) (Cell Signaling), anti- α-smooth muscle actin (α-SMA) (clone 1A4, 1:200) (Dako), anti-Ki-67 (rabbit polyclonal, ab15580, 1:300) (Abcam), anti-CD105-PE (clone SN6, 1:100) (eBioscience), anti-mitochondria (clone 113-1, 1:100) and anti-fibroblast specific protein-1 (FSP-1; rabbit polyclonal, 07-2274, 1:200) (Millipore). IL-6 ELISA kit was purchased from BD Biosciences. CCL5 DuoSet ELISA kit and mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit were from R&D Systems. Alexa Fluor® fluorescent dye-conjugated secondary antibodies were from Life Technologies for immunofluorescent staining.
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