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Scopolamine hbr

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Scopolamine HBr is a compound used in various laboratory applications. It is a salt form of the naturally occurring alkaloid scopolamine. Scopolamine HBr is commonly utilized as a reference standard in analytical procedures, but its specific applications may vary depending on the research or testing requirements.

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Lab products found in correlation

Epinephrine bitartrate, by Merck Group (1 mentions) Atropine sulfate, by Merck Group (1 mentions) Lidocaine hcl, by Merck Group (1 mentions) Phentolamine, by Merck Group (1 mentions) Phencyclidine, by Merck Group (1 mentions) 3h spiperone, by PerkinElmer (1 mentions) Isobutylmethylxanthine, by Merck Group (1 mentions) D amphetamine sulphate, by Merck Group (1 mentions) 35s gtpγs, by PerkinElmer (1 mentions) Methysergide maleate, by Sandoz (1 mentions) Rolipram, by Merck Group (1 mentions)

4 protocols using scopolamine hbr

1

Pharmacological Evaluation of Plant Extracts

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The following drugs and dosage were used: carbachol HCl (Sigma) 2 mg/kg; atropine sulfate (Sigma) 2.5 mg/kg; scopolamine HBr (Sigma) 2 mg/kg; epinephrine bitartrate (Sigma) 1 mg/kg; amphetamine bitartrate 5 mg/kg; lidocaine HCl (Sigma) 40 mg/kg; acetyl salycilic acid,100 mg/kg; morphine HCL, 10 mg/kg; haloperidol (Syntex latino, SA) 1 and 6 mg/kg. Each drug was dissolved in isotonic saline solution (0.9% NaCl) just before use, and the resulting solution was administered by intraperitoneal (i.p.) injection of 0.2 mL. The hydroalcoholic extract of the bark was administered intraperitoneally at the dose of 0.5 g dried plant/kg weight, and dichloromethane extract at 1.25 g dried plant/kg weight.
de la Cabeza Fernández M., Sánchez M., Caceres A., Iglesias I, & Gómez-Serranillos M.P. (2023). Neuropharmacological Effects in Animal Models and HPLC-Phytochemical Profiling of Byrsonima crassifolia (L.) Kunth Bark Extracts. Molecules, 28(2), 764.
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2

Scopolamine-HBr Spatial Memory Study

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Scopolamine-HBr was purchased from Sigma-Aldrich (St Louis, MO) and saline (0.9% NaCl) was from Hospira (Lake Forest, IL). Scopolamine-HBr was dissolved in saline and freshly prepared each day animals were dosed. The Morris water maze pool was purchased from Harvard Apparatus (Holliston, MA); all other testing equipment was purchased from Stoelting Co. (Wood Dale, IL).
Wolf A., Bauer B., Abner E.L., Ashkenazy-Frolinger T, & Hartz A.M. (2016). A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice. PLoS ONE, 11(1), e0147733.
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3

Radioligand Binding Assay Protocol

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The following radioligands were purchased from Amersham Bioscience: [3H]prazosin [furanyl-5-3H] (TRK-843, specific activity 88 Ci/mmol), [3H]mesulergine [N6-methyl-3H] (TRK-845, specific activity 84 Ci/mmol), and [35S]GTPγS (SJ-1308, specific activity 1000–1200 Ci/mmol).
The following ligands were purchased from PerkinElmer Inc.: [3H]spiperone (NET-565, specific activity 15.7 Ci/mmol), [3H]8-OH-DPAT (NET-929, specific activity 129 Ci/mmol), and [3H]ketanserin (NET-791, specific activity 88.0 Ci/mmol).
[3H](+)PHNO (specific activity 63–64 Ci/mmol) was purchased from Moravek Biochemical Inc. (Brea, CA, USA).
Apomorphine HCl, butaclamol HCl, D-amphetamine sulphate, forskolin (FSK), phencyclidine, phentolamine, scopolamine HBr, (±)-2,5-dimethoxy-4-iodoamphetamine HCI, serotonin-creatinin sulphate, and isobutyl-methyl xanthine were purchased from Sigma-Aldrich Co. (St Louis, MO, USA).
Mianserin HCl was obtained from Organon, methysergide maleate from Sandoz, and MK-801 from RBI. Haloperidol, cariprazine, DDCAR (as base or HCl salt), and DCAR (as base or HCl salt) were synthesized at Gedeon Richter Plc (Budapest, Hungary).
Other chemicals used in the experiments were of analytical grade and obtained from commercial sources.
Kiss B., Némethy Z., Fazekas K., Kurkó D., Gyertyán I., Sághy K., Laszlovszky I., Farkas B., Kirschner N., Bolf-Terjéki E., Balázs O, & Lendvai B. (2019). Preclinical pharmacodynamic and pharmacokinetic characterization of the major metabolites of cariprazine. Drug Design, Development and Therapy, 13, 3229-3248.
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4

Rodent Cognitive Enhancement Protocol

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Each compound was freshly dissolved on every experimental day. Donepezil (1 mg/kg; generously given by Solvay Pharmaceuticals, Weesp, The Netherlands) was dissolved in saline and administered p.o., the injection volume was 1 mL/kg. Vardenafil (1 mg/kg; kindly donated by BAYER AG, Wuppertal, Germany) was dissolved in a vehicle containing 98% tylose (methyl-cellulose) solution (1%) and 2% tween 80, and was given orally (p.o.) at an injection volume of 1 mL/kg. The same vehicle was used for dissolving rolipram, which was given i.p. (1 mL/kg). For rolipram (Sigma-Aldrich, Zwijndrecht, the Netherlands), a dose of 0.03 mg/kg was used in the 24-h retention interval sessions and 0.1 mg/kg in the 1-h retention sessions in combination with scopolamine. A fixed dose of 0.1 mg/kg scopolamine Hbr (Sigma-Aldrich) was used as an acquisition-deficit model, and was also dissolved in saline. Scopolamine was administered intraperitoneally (i.p.) at a volume of 1 mL/kg. Doses, vehicles, and administration routes were determined based on earlier studies using these drugs (Prickaerts et al. 2005 (link); Rutten et al. 2006 (link), 2007 (link)).
Akkerman S., Blokland A, & Prickaerts J. (2016). Possible overlapping time frames of acquisition and consolidation phases in object memory processes: a pharmacological approach. Learning & Memory, 23(1), 29-37.
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