Petri dishes

Manufactured by Techno Plastic Products

Sourced in Switzerland

Petri dishes are circular, shallow glass or plastic containers commonly used in laboratories for culturing microorganisms. They provide a contained environment for growing and observing microbial cultures.

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Lab products found in correlation

3 protocols using petri dishes

Cellular Uptake of AuNPs in 2D and 3D

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Cells of the HepG2 and HEK293 lines were sown in Petri dishes (40 mm diameter, TPP Techno Plastic Products AG, Trasadingen, Switzerland), 10⁵ cells per dish. After reaching 70% coverage, the monolayers were washed with a culture medium, and AuNPs or AuBSA-NPs, or AuPEI-NPs suspended in an IMDM (for HepG2) or DMEM (for HEK293) were added to cells. Final concentration of AuNPs in the medium was 1 nM.
The cells were incubated for 15 min, 30 min, 1, 2 and 4 h in a medium without serum. Then the cells were rinsed three times with PBS, removed with trypsin, sedimented by centrifugation (5 min at 3000 rpm), and fixed with 4% paraformaldehyde for TEM studies.
Seven-day spheroids of HepG2 and HEK 293 cells cultured in 96-well plates were washed with a culture medium. The AuNPs or AuBSA-NPs or AuPEI-NPs were added to spheroids in an IMDM (HepG2-spheroids) or DMEM (HEK293 spheroids); final concentration of AuNPs was 1 nM. The spheroids were incubated with NPs for 1, 2 and 4 h without serum, and then fixed with 4% paraformaldehyde.

Chelobanov B., Poletaeva J., Epanchintseva A., Tupitsyna A., Pyshnaya I, & Ryabchikova E. (2020). Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids. Nanomaterials, 10(10), 2040.

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Culturing CHO Cell Lines

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CHO (CCL-61T; ATCC, Teddington, UK) cell lines were cultured on Petri dishes (Techno Plastic Products, Neuchâtel, Switzerland), coated with poly-D-lysine (PDL; Sigma-Aldrich, Milano, Italy), in Dulbecco’s modified Eagle’s medium (Gibco, Paisley, UK) containing 4.5% glutamine and glucose, 10% inactivated fetal bovine serum, 1.0% penicillin-streptomycin, and 1.0% nonessential amino acids (Gibco) at 37 °C in 5.0% CO₂. The cells were split every 4–5 days before reaching a confluency rate of <80%.

Oropesa-Nuñez R., Mescola A., Vassalli M, & Canale C. (2021). Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature. Sensors (Basel, Switzerland), 21(4), 1069.

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Adipogenic Differentiation of hADMSCs

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Human adipose-derived mesenchymal stem cells (hADMSCs), isolated from a female donor from subcutaneous adipose tissue and characterized by flow cytometry were purchased from Thermo Fisher (Thermo Fisher Scientific, Carlsbad, CA, USA) and cultivated as described previously (Mullerova et al. 2017) . Briefly, the cells were seeded at 1×10 5 cells and cultured in Petri dishes (TPP Techno Plastic Products, Trasadingen, Switzerland) in commercially available culture medium MesenPRO RS™ Medium supplemented with MesenPRO RS™ Growth Supplement with reduced serum level (2 %), 1 % L-glutamine and 1 % gentamicin (all Thermo Fisher Scientific, Carlsbad, CA, USA).
After 80 % confluence was reached, the hADMSCs were cultured for adipogenic differentiation in culture plates according to the manufacturer's instructions in StemPro® Adipogenesis Differentiation Basal Medium (DM) with StemPro® Adipogenesis Supplement and 1 % gentamicin (all Thermo Fisher Scientific, Carlsbad, CA, USA). The medium was changed every 3 days up to a total incubation time of 21 days. The cells were maintained and cultured into differentiated adipocytes under 5 % CO 2 atmosphere at 37 °C.

Kladnická I., Čedíková M., Kripnerová M., Dvořáková J., Kohoutová M., Tůma Z., Müllerová D, & Kuncová J. (2019). Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiological research, 68(Suppl 3).

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