Potter s
The Potter S is a laboratory homogenizer designed for the disruption and homogenization of biological samples. It features a motorized pestle that operates in a glass or PTFE tissue grinder vessel to efficiently break down and mix various materials.
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10 protocols using potter s
Isolation of Rat Brain and Liver Mitochondria
Myeloperoxidase Activity Assay in Ileum
The method of Suzuki et al. was used with a slight modification [13 (link)]. This method is based on the oxidation of the synthetic substrate 3,3’,5,5’-tetramethyl benzidine (TMB) by MPO. The standard reaction mixture consisted of 500 μl detergent-containing buffer (160 mM potassium phosphate buffer, pH 5.4, 1% hexadecyltrimethylammonium bromide), 100 μl TMB (16 mM, dissolved in dimethylformamid), 50 μl homogenate and 300 μl water. The reaction was started by the addition of 50 μl H2O2 (diluted 0.003%) at 37°C. The rate of MPO-catalyzed oxidation of TMB was followed by recording the increase in absorbance at 655 nm. Considering the initial and linear phase of the reaction, we measured the absorbance change per minute, and one enzyme unit was defined as the amount of enzyme producing one absorbance change per minute under assay conditions. Enzyme activity was calculated as units per gram of wet weight tissue.
Subcellular Fractionation of SERT Cells
Acetylcholinesterase Activity Quantification
Affinity Isolation of Endogenous PKA Complexes
Isolation of Mitochondria from Liver Tissue
Liver Extraction and Homogenization Protocol
Isolation of Endogenous PKA Complexes
Postmortem Brain Tissue Homogenization
Fractionation of Cerebellar Proteins
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