All animal protocols were approved by the Institutional Animal Care and Use Committee at Massachusetts General Hospital (Protocols #2009N000239 and #2013N000115). The following mouse lines were obtained from Jackson Laboratory (Bar Harbor, ME, USA): TauGFP/GFP mice, Ednrb+/− mice, Wnt1::Cre mice, R26-iDTR mice, and PC::G5-tdT mice (Supplementary Table S1 ). Plp1-GFP mice were kindly gifted by Dr Wendy Macklin, University of Colorado.22 (link) The various breeding schemes and genotypes of controls are summarized in Table 1 . Wnt1::Cre mice were crossed with R26-iDTR mice to obtain Wnt1-Cre;R26-iDTR (Cre+) mice (hereafter referred to as Wnt1-iDTR). R26-iDTR mice (Cre-) were used as control.
R26 idtr mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
The R26-iDTR mice are a transgenic mouse model that expresses a diphtheria toxin receptor (iDTR) under the control of the ROSA26 promoter. This model allows for the inducible and specific ablation of targeted cell populations upon administration of diphtheria toxin.
Automatically generated - may contain errors
4 protocols using r26 idtr mice
1
Genetic Mouse Models for Neural Crest Studies
2
Genetically Engineered Mouse Lines
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All animal protocols were approved by the Institutional Animal Care and Use Committee at Massachusetts General Hospital (protocols #2009N000239 and #2013N000115). All methods were performed in accordance with relevant regulations. The following mouse lines were obtained from Jackson Laboratory (Bar Harbor, ME): ChAT::Cre mice (stock #006410) were crossed with R26-iDTR mice (stock #007900) to obtain ChAT::Cre;R26-iDTR (annotated as ChAT-iDTR) mice. ChAT::Cre mice also were crossed with R26-ChR2-tdTomato (stock #012567) reporter mice to obtain ChAT::Cre;R26-ChR2-tdTomato (annotated as ChAT-ChR2) mice, in which ChAT+ cells express the light-sensitive ion channel ChR2 and red fluorescent protein, tdT. Cx3cr1GFP;CCR2RFP mice (stock #032127), in which red fluorescent protein is expressed by peripheral monocytes and lymphocytes, and green fluorescent protein is expressed by macrophages. All experiments used adult mice (both sexes) between 10 and 12 weeks of age.
3
Conditional Diphtheria Toxin Ablation
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All animal protocols were approved by the Institutional Animal Care and Use Committee at Massachusetts General Hospital (Protocols #2009N000239 and #2013N000115). All methods were carried out in accordance with relevant guidelines and regulations. The reporting in the manuscript follows the recommendations in the ARRIVE guidelines.
The following mouse lines were obtained from Jackson Laboratory (Bar Harbour, ME, USA): Wnt1::Cre mice (Stock #009107) were crossed with R26-iDTR mice (Stock #007900) to obtain Wnt1::Cre; R26-iDTR (Wnt1-iDTR) mice. R26-iDTR mice were used as controls. All mice were used at 2–3 months of age and included both sexes.
The following mouse lines were obtained from Jackson Laboratory (Bar Harbour, ME, USA): Wnt1::Cre mice (Stock #009107) were crossed with R26-iDTR mice (Stock #007900) to obtain Wnt1::Cre; R26-iDTR (Wnt1-iDTR) mice. R26-iDTR mice were used as controls. All mice were used at 2–3 months of age and included both sexes.
4
Genetically Modified Mice in Bone Research
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All animals were maintained under specific pathogen-free conditions, and all experiments were performed with the approval of the Institutional Review Board at The University of Tokyo. C57BL/6 mice were purchased from CLEA Japan. KSN/Slc Nude mice (6-8 week-old male) were purchased from SLC Japan. R26-iDTR mice, Ihhflox/flox mice and B6.SJL (CD45.1+) mice were obtained from the Jackson Laboratory. Prmt5flox/flox mice12 (link), Ctsk-Cre mice14 (link), CAG-CAT-EGFP mice37 (link), Col2a1-Cre mice18 (link), Sp7-Cre mice19 (link), LysM-Cre mice17 (link), Col10a1-Cre mice30 (link) and Tnfsf11flox/flox mice28 (link) were described previously. Three-dimensional microcomputed tomography analyses and bone morphometric analyses were performed as described38 (link). Age and sex-matched littermates were used for all of the experiments unless otherwise noted. The sex and age of mice used are described in figures or figure legends. All animals were maintained at a constant ambient temperature of 22–26 degree Celsius, 40–65% of humidity under a 12 h light/dark cycle with free access to food and drink.
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