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Linoleoyl coa

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Linoleoyl-CoA is a chemical compound that serves as an essential substrate in various metabolic processes. It is a fatty acyl-coenzyme A derivative that plays a crucial role in the regulation of lipid metabolism. As a core function, Linoleoyl-CoA is involved in the synthesis and degradation of fatty acids, contributing to the overall energy homeostasis of the cell.

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Lab products found in correlation

Oleoyl coa, by Merck Group (2 mentions) Qubit protein assay kit, by Thermo Fisher Scientific (1 mentions) Labchip ez reader 12 sipper chip, by PerkinElmer (1 mentions) Profilerpro separation buffer, by PerkinElmer (1 mentions) Palmitoleoyl coa, by Merck Group (1 mentions) Myristoyl coa, by Merck Group (1 mentions) Eicosapentaenoic acid, by Merck Group (1 mentions) Clofibrate, by Merck Group (1 mentions) Anti rabbit igg secondary antibody, by Merck Group (1 mentions) Palmitoyl coa, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Linoleic acid, by Merck Group (1 mentions) Coenzyme a, by Merck Group (1 mentions)

3 protocols using linoleoyl coa

1

Diacylglycerol Acyltransferase Activity Assay

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The diacylglycerol acyltransferase activity assay used was modified from Sanderson et al.38 (link) and performed as described by Haili et al.39 (link) using a reaction mixture containing 60 µg of the purified enzyme in 100 mM phosphate buffer pH 8 and 100 µM 1,2-Dioleoyl-sn-glycerol (Cayman chemical, Ann Arbor, USA) in a final volume of 100 µl. The reaction mixture was incubated with 50 µM linoleoyl-CoA (Sigma-Aldrich). The Dga1p∆19 protein from Y. lipolytica, used as positive control, was obtained according to Haili et al.39 (link). Substrates and products were separated on HPTLC plates and lipids visualized using methanolic cupric sulfate oxidation according to the same authors39 (link).
Aymé L., Arragain S., Canonge M., Baud S., Touati N., Bimai O., Jagic F., Louis-Mondésir C., Briozzo P., Fontecave M, & Chardot T. (2018). Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis. Scientific Reports, 8, 17254.
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2

Purification and Characterization of Acyl-CoA Analogues

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Recombinant p300 (1195-1662) and Gcn5 (497-662) were obtained from Enzo. Acetyl-CoA, propionyl-CoA, butyryl-CoA, succinyl-CoA, crotonyl-CoA, malonyl-CoA, and palmitoyl-CoA were synthesized according to the literature procedure (Padmakumar et al., 1997 (link)). All synthesized CoA analogues were HPLC purified, with purity verified by LC-MS prior to use. Linoleoyl-CoA, myristoyl-CoA, oleoyl-CoA, and palmitoleoyl-CoA were purchased from Sigma with purity verified by LC-MS prior to use. H3K14-CoA and desulfo-CoA were synthesized according to previously reported procedures (Chase et al., 1966 (link); Montgomery et al., 2014 (link); Zheng et al., 2004 (link)). Qubit Protein Assay kit (Life Technologies) was used to determine cell lysate and histone extract concentrations. Pyruvate dehydrogenase, ketoglutarate dehydrogenase, and NAD+ were purchased from Sigma. Labchip EZ-Reader 12-sipper chip (#760404) and ProfilerPro Separation Buffer (#760367) were purchased from Perkin-Elmer. H3K9Ac (9649P), H3K14Ac (7627P), H3K27Ac (8173), H4K8Ac (2594P), acetylated tubulin (5225P), GAPDH (5174S), Gcn5 (3305S), and pCAF (3378S) antibodies were purchased from Cell Signaling Technologies, while Mof (A300-992A-T) antibody was purchased from Bethyl Laboratories.
Montgomery D.C., Sorum A.W., Guasch L., Nicklaus M.C, & Meier J.L. (2015). Metabolic Regulation of Histone Acetyltransferases by Endogenous Acyl-CoA Cofactors. Chemistry & biology, 22(8), 1030-1039.
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3

Fatty Acid Biosynthesis Regulation

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Coenzyme A, palmitic acid, oleic acid, linoleic
acid, eicosapentaenoic acid, palmitoyl-CoA, oleoyl-CoA, linoleoyl-CoA,
and clofibrate were from Sigma (St. Louis, MO). Eicosapentaenoyl-CoA
was synthesized as previously described15 (link) and purified by high-performance liquid chromatography (HPLC).17 (link) All CoA thioesters, whether freshly synthesized
or obtained commercially, were >98% undegraded. The human glucocorticoid
receptor (hGR) was purchased from Pierce Thermo Scientific (Rockford,
IL). Monoclonal antibodies for PPARα and polyclonal antibodies
for LXRα (each specific for the α isotype) were purchased
from Pierce Thermo Scientific. Polyclonal antibodies for PPARα,
RXRα, and GR were purchased from Santa Cruz Biotechnology (Santa
Cruz, CA). Anti-rabbit IgG secondary antibodies were from Sigma.
Balanarasimha M., Davis A.M., Soman F.L., Rider SD J.r, & Hostetler H.A. (2014). Ligand-Regulated Heterodimerization of Peroxisome Proliferator-Activated Receptor α with Liver X Receptor α. Biochemistry, 53(16), 2632-2643.
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