The experimental set-up consisted of a PVM lodged onto a 3D printed inclined platform (inclination angle of 25°), to replicate patient's leg elevation as in the clinical procedure. The inlet tube was connected to the PVM using a three-way stopcock. A blood substitute (30% v/v glycerol in purified water) with a fluid dynamic viscosity, μ, of 0.003 Pa × sec and density, ρ, of 1,078 kg/m3 (Pries et al., 1992 (link)) was conveyed through the vein model using a 10 mL syringe (BD Biosciences, USA). A steady flow of the blood substitute was imposed using a syringe pump (NE-1000 Programmable Single Syringe Pump, New Era Pump Systems, Inc., USA). A pressure transducer (Research Grade Blood Pressure Transducer, 230 VAC, 50 Hz, Harvard apparatus, UK) was positioned in line with the inlet tubing, and located 30 mm proximally to the PVM inlet (Figure 3 ). The pressure transducer was connected to a National Instruments I/O module (NI-DAQ, USB-6008, National Instrument, UK). The NI-DAQ system supports analog and digital inputs, and communicates with the NI-DAQ software (National Instrument, UK). A MATLAB® (The MathWorks Inc., USA) script was employed to store pressure data in an automated fashion.
Ne 1000 programmable single syringe pump
Manufactured by New Era Pump Systems
Sourced in United States
The NE-1000 Programmable Single Syringe Pump is a laboratory equipment product designed to precisely dispense and infuse liquids. It features a programmable microprocessor control system that allows for customizable flow rates and infusion volumes.
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Lab products found in correlation
5 protocols using ne 1000 programmable single syringe pump
1
Evaluating Venous Flow Dynamics
2
Wireless Cortical Recording in Gerbils
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Trained gerbils were tested while cortical potentials were simultaneously recorded, amplified and transmitted wirelessly to a receiver positioned approximately 1 m from the cage (W16, Triangle BioSystems International). All input/output channels were synchronized at 100,000 samples/s and 16 bits by sharing the sampling clock pulse of two data acquisition cards (DAQs, PCIe-6321 and PCIe-6341, National Instruments Corporation) via a Real-Time System Integration bus cable. Specifically, custom written software, called Electrophysiology Auditory Recording System (EARS), synchronously controlled auditory stimuli delivery and recorded both behavioral and physiological responses23 . EARS communicated with the loudspeaker, nose poke, licks spout, W16 and a personal computer for data storage and analysis via the two DAQs. In addition, EARS interfaced with a syringe pump (NE-1000 Programmable Single Syringe Pump, New Era Pump Systems, Inc.) via a USB-RS232 emulator.
3
Measuring GUV S-parameters Using Syringe Pump
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An NE-1000 Programmable Single Syringe Pump (New Era Pump Systems Inc., Farmingdale, NY, USA) is used to push GUVs into and out of the channel. A 2 μL/min flow rate is set in all experiments. A vector network analyzer (VNA) for S-parameter measurement is connected to a laptop and operated with LabView and graphical codes (National Instruments Corporation, Austin, TX, USA). The measured real and imaginary parts of S21 are saved with LabView codes. A 1 kHz intermediate frequency bandwidth (IFBW) is used for VNA measurement.
4
Urodynamic Evaluation of Bladder Capacity
5
Electrospinning of PCL/Poloxamer Fiber Meshes
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All PCL/poloxamer fibers were electrospun within 48 h of the solution preparation. Prior to electrospinning, polymer solution was drawn into a 3 mL Luer-LokTM BD syringe (Franklin Lakes, NJ, USA) that was attached to a 21-gauge blunt needle. The syringe and needle assembly were then placed onto a NE-1000 programmable single syringe pump (New Era Pump Systems Inc., Farmingdale, NY, USA) to deliver polymer solution at a flow rate of 25 μL/min. During electrospinning, the applied voltage was set at 10 kV over a distance of 10 cm between the tip of the needle and the grounded stationary collector covered with a layer of wax paper. A total of 2 mL of the polymer solution was electrospun from all polymer solutions, recorded by the programmable syringe pump, which was regularly calibrated. After each electrospinning, fiber meshes were covered with aluminum foils and stored in a vacuum desiccator to further remove excess solvents before fiber characterizations.
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