(Heidelberg, Germany). The hFDPCs were cultured in FDPC growth medium
supplemented with 4% (v/v) fetal calf serum, 0.4% (v/v) bovine pituitary
extract, human recombinant basic fibroblast growth factor (1 ng/mL), and human
recombinant insulin (5 μg/mL; PromoCell). Human hair germinal matrix
cells (hGMCs) were obtained from ScienCell Research Laboratories (Carlsbad, CA,
USA). The hGMCs were cultured in mesenchymal stem cell medium supplemented with
5% (v/v) fetal bovine serum, and 1% (v/v) mesenchymal stem cell growth
supplement (ScienCell Research Laboratories). Both hFDPCs and hGMCs were
cultured at 37°C in a humidified atmosphere of 5 % CO2.