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Human tnfα elisa ready set go

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The Human TNFα ELISA Ready Set Go is a sandwich enzyme-linked immunosorbent assay (ELISA) kit designed for the quantitative detection of human tumor necrosis factor alpha (TNFα) in biological samples. The kit includes all the necessary reagents and components to perform the assay.

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Lab products found in correlation

Human il 1β elisa, by Thermo Fisher Scientific (1 mentions) Human il 18 platinum elisa, by Thermo Fisher Scientific (1 mentions) Spectamax m5, by Molecular Devices (1 mentions)

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ELISA Ready-SET-Go (110 citations) Ready-SET-Go (98 citations) ELISAs (35 citations) TNFα ELISA (13 citations)

2 protocols using human tnfα elisa ready set go

1

Rivaroxaban and Fondaparinux Modulate IL-8 and TNFα

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IL-8 secretion level was measured by ELISA (kit Human CXCL8/IL-8 Quantikine® R&D Systems) in the supernatants of non-activated and activated THP-1 incubated with defibrinated plasma in conditions described above, in absence or presence of rivaroxaban at 0.35 μg/ml and 1.5 μg/ml fondaparinux. TNFα concentration in the supernatants was determined using Human TNFα ELISA Ready Set Go (eBioscience®) according to the manufacturer’s instructions.
Laurent M., Joimel U., Varin R., Cazin L., Gest C., Le-Cam-Duchez V., Jin J., Liu J., Vannier J.P., Lu H., Soria J., Li H, & Soria C. (2014). Comparative study of the effect of rivaroxaban and fondaparinux on monocyte’s coagulant activity and cytokine release. Experimental Hematology & Oncology, 3, 30.
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2

Quantification of Inflammatory Cytokines

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Human IL-18, IL-1β, and TNF-α were measured with the Human IL-18 Platinum ELISA, Human IL-1β ELISA and Human TNF-α ELISA Ready-SET-Go kits respectively (eBiosciences, San Diego CA) using 50 µL of plasma or 100 µL of culture supernatant. Each sample was tested in duplicate. Data was acquired using a SpectaMax M5 (Molecular Devices, Sunnyvale CA). The LLOD of IL-18 was 40 pg/mL in plasma and 18 pg/mL in culture supernatant.
Chattergoon M.A., Latanich R., Quinn J., Winter M.E., Buckheit RW 3.r.d., Blankson J.N., Pardoll D, & Cox A.L. (2014). HIV and HCV Activate the Inflammasome in Monocytes and Macrophages via Endosomal Toll-Like Receptors without Induction of Type 1 Interferon. PLoS Pathogens, 10(5), e1004082.
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