Cytarabine

Five juvenile BALB/c mice (1‐3 days old) were individually sacrificed in 75% ethanol at −20°C. The DRGs were extracted and placed in Dulbecco's modified Eagle's medium DMEM/F12 culture medium cooled on ice. The DRGs were completely shredded and digested with 0.25% trypsin and 0.1% type‐4 collagenase. followed by mixing and incubation at 37°C in a humidified atmosphere comprising 5% CO₂ for 30 minutes to dissolve the tissue and release single cells. The process was assessed every 5 minutes, with gentle shaking to improve the mixing of the tissue pieces and the digestion solution. A fivefold volume of DMEM/F12 medium with 20% FBS was added to the centrifuge tube and allowed to equilibrate for 5 minutes without agitation to stop the digestion. Then, the cells were centrifuged at 300 × g for 5 minutes and resuspended in neurobasal serum‐free medium with B27 and 1% penicillin/streptomycin solution added. The cells were plated in 6‐well plates at 1 × 10⁴. After 24 hours, the medium was exchanged again, and a solution comprising 5 µmol/L cytarabine (MedChemExpress, Monmouth Junction, NJ), B27, 1% penicillin/streptomycin solution, and Neurobasal serum‐free medium was added. This medium was exchanged for normal culture medium after another 48 h.

Rilpivirine, etravirine, and cytarabine were purchased from MedChem Express (Monmouth Junction, NJ, USA) or Targetmol (Boston, MA, USA). All purchased drugs had reported purity of ≥95% from the commercial suppliers. Resazurin and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). All drug solutions were prepared as 10 mM stocks with 100% DMSO and stored at −20 °C.

PLX51107 was kindly provided by Plexxikon or purchased from MedChemExpress. Dinaciclib, cytarabine, etoposide, IWR-1, BML-284, and CHIR-99,021 (laduviglusib) were obtained from MedChemExpress. All chemical compounds were dissolved in Dimethyl Sulfoxide (DMSO) to prepare a 10 mM stock solution and were stored at -80 C for long-term storage as recommended.