Uv 2250

Manufactured by Shimadzu

Sourced in Japan

The UV-2250 is a compact and high-performance UV-Visible spectrophotometer manufactured by Shimadzu. It is designed to provide accurate and reliable measurements across a wide range of wavelengths, from 190 to 1100 nanometers. The UV-2250 features a deuterium and tungsten-halogen light source, allowing for a broad spectrum analysis. It is equipped with a double-beam optical system and a high-resolution monochromator, ensuring precise and reproducible results.

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Lab products found in correlation

Perfection v700 photo, by Epson (1 mentions) Dr 2800 spectrophotometer, by HACH (1 mentions) Toc vcph, by Shimadzu (1 mentions) Gcms qp2010, by Shimadzu (1 mentions) Verios 460, by Thermo Fisher Scientific (1 mentions) Tecnai 12, by Thermo Fisher Scientific (1 mentions) Supra 35 vp sem, by Zeiss (1 mentions) Ftir spectrophotometer, by PerkinElmer (1 mentions) D8 diffractometer, by Bruker (1 mentions) Vertex 70, by Bruker (1 mentions) Zetasizer nano zs90, by Malvern Panalytical (1 mentions)

Spelling variants of this product

UV-2250 spectrophotometer (9 citations) UV-2450/2250 (4 citations) UV-2250 UV—VIS spectrophotometer (3 citations)

9 protocols using uv 2250

Leaf Nitrogen Allocation and Chlorophyll Determination

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After determination of the gas exchange parameters and fluorescence yield, leaf samples and nearby leaves (30–50 leaves in total per shoot), were taken from each shoot. The surface area of 10–20 leaves was measured by scanner (Perfection v700 Photo, Epson, Nagano-ken, Japan). Leaves were subsequently oven-dried at 80°C for 48 h to constant weight, dry weight was measured using an analytic balance, and then LMA was calculated. Dried leaf samples were ground into a dry flour, nitrogen concentration was determined by a VELP automatic Kjeldahl nitrogen determination apparatus (UDK-139, Milano, Italy), and then leaf nitrogen per mass (N_mass) and leaf nitrogen per area (N_area) were calculated.
The remaining 20–30 leaves were frozen and returned for laboratory analysis. One gram of frozen leaves (5–10 leaves) were cut into small pieces and weighed into 5–10 mg samples. Absolute chlorophyll concentration measurements were conducted using 95% (v/v) alcohol extracts of leaf tissue and a Shimadzu visible-ultraviolet spectrophotometer (UV 2250, Fukuoka, Japan), chlorophyll concentration see S2 Table. The remaining frozen leaves were used to determine cell wall nitrogen content according to Onoda et al. [7 ]. The fraction of leaf nitrogen allocated to cell walls (P_CW) represents the ratio of cell wall nitrogen content to total nitrogen content.

Tang J., Cheng R., Shi Z., Xu G., Liu S, & Centritto M. (2018). Fagaceae tree species allocate higher fraction of nitrogen to photosynthetic apparatus than Leguminosae in Jianfengling tropical montane rain forest, China. PLoS ONE, 13(2), e0192040.

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Comprehensive Characterization of CGW

Cited 1 time

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The organic compounds in the CGW were analyzed using gas chromatography-mass spectrometry (Shimadzu GCMS-QP2010, Japan) equipped with a DB-5ms capillary column (50 m × 0.25 mm ID × 0.25 μm). The temperature for the GC-MS interface and ion source were 250 °C and 220 °C, respectively. The mass spectrometer was operated in the electron ionization (EI) mode at 70 eV and helium (flow rate of 1 ml min⁻¹) was used as carrier gas. The injection port was maintained at 300 °C. The column temperature was held at 80 °C for 1 min, and then programmed at 12 °C/min to 200 °C (hold 1 min), 8 °C/min to 280 °C (hold 15 min), then at 2 °C/min to 300 °C. The total organic carbon (TOC), which is used to represent the total concentration of contaminants in CGW, was determined using a TOC analyzer (Shimadzu TOC-VCPH, Japan) after dilution of the samples. The concentration of H₂O₂ and persulfate were measured using the iodide-spectrophotometric method described by Zhong et al [30 (link)]. The concentration of permanganate ion (MnO₄⁻) was measured with a UV-spectrophotometer (UV-2250, Shimadzu) at a wavelength of 525 nm [69 (link), 70 (link)]. Sulfate, ferrous ion and total dissolve iron were measured using a Hach DR2800 spectrophotometer (Loveland, CO) based on the modulated Hach methods (No. 8051, 8146, 8008). The pH of samples was determined using PHS-3C pH meter (Leici Instrument Co., Shanghai).

Zhong H., Tian Y., Yang Q., Brusseau M.L., Yang L, & Zeng G. (2016). Degradation of landfill leachate compounds by persulfate for groundwater remediation. Chemical engineering journal (Lausanne, Switzerland : 1996), 307, 399-407.

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Comprehensive Material Characterization

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The morphology was characterized
by transmission electron microscopy (TEM, Tecnai 12, FEI, Netherlands)
and field emission scanning electron microscopy (SEM, FEI, Verios
460), while the powder diffraction patterns were performed by X-ray
polycrystalline powder meter (XRD, Rigaku, Ulitma IV). The absorption
spectrum was performed on a UV–visible spectrophotometer (UV-2250,
Shimadzu), while the functional groups were characterized by Fourier
transform infrared (FTIR) spectroscopy (Thermo Fisher, IS10). The
specific surface area and pore size were acquired by an automatic
multi-station specific surface and microporous mesoporous physical
adsorption instrument (BET, MIKE, Gemini VII 2390). High resolution
electrospray ionization mass spectroscopy (FT-MS, Bruker solanX 70,
Agilent 6540TOF) was used to obtain the m/z of the degradation products. The elemental valence states
were characterized by X-ray photoelectron spectroscopy (XPS, Thermo
Scientific K-Alpha).

Zhang Q., Qileng A., Li J., Cao Y., Liu W, & Liu Y. (2023). Grafting a Porous Metal–Organic Framework [NH2-MIL-101(Fe)] with AgCl Nanoparticles for the Efficient Removal of Congo Red. ACS Omega, 8(5), 4639-4648.

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Cr(VI) Quantification Using UV-Vis

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A stock solution containing Cr(VI) 500 mg/L was prepared by dissolving 0.7206 g of potassium dichromate with distilled water in a 500 mL of measuring flask and diluted to desired concentration, the pH of Cr(VI) solution were adjusted to desired value with 0.1 M of HNO₃ and NaOH solution under the pH meter (Jingke, Shanghai Co. Ltd). Concentration of Cr(VI) was determined using a Ultraviolet-visible spectrophotometer (UV-2250, SHIMADZU, Japan), which analyzed through the purple red complex with 1, 5-diphenylcarbazide in acid medium at λ = 540 nm. The standard curves of Cr(VI) concentration was obtained through determining the absorbance of known concentration of Cr(VI) with 0–2.0 mg/L (seen in Fig. 4), the linear equation of absorbance (A) versus Cr(VI) concentration (C, mg/L) was calculated as follow: A = 0.31693 C + 0.00296, the scope of A is 0–0.7 and the correlation coefficient of the standard curve is 0.9973.

Liu H., Zhang F, & Peng Z. (2019). Adsorption mechanism of Cr(VI) onto GO/PAMAMs composites. Scientific Reports, 9, 3663.

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Characterization of TiO2-Nanocomposite Adsorbent

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The prepared adsorbents were characterized by different analytical techniques, the elemental composition was studied through EDX. The prepared TiO₂ NPs were confirmed through the UV-visible spectrophotometer (UV-2250, Shimadzu, Tokyo, Japan). Then, 50 mg of BC was sonicated with ethanol and then TiO₂ NPs were added to dispersed BC. The developed dispersed mixture was ultrasonicated for 3 h followed by drying at moderate conditions to remove the solvent. After drying, the prepared nanocomposite was deposited on a petri dish and subjected to microwave irradiation for 2 min to give better exfoliation [25 (link)]. XRD technique (BTX III Benchtop XRD Analyzer, Olympus Corporation, Tokyo, Japan) was used to find if adsorbents had crystalline structure. Particle size was calculated through Scherrer’s formula, which is given as:

D = K \frac{λ}{β \cos θ}

where, K is a constant, λ is the wavelength of radiation and β is the full width half maximum.
Functional groups were determined through FT-IR (Perkin Elmer FT-IR Spectrophotometer, Waltham, MA, USA). The surface morphology of biosorbents before and after the experiment was studied by SEM (Carl Zeiss AG-SUPRA 35 VP SEM, Oberkochen, Germany).

Ashfaq A., Nadeem R., Gong H., Rashid U., Noreen S., Rehman S.U., Ahmed Z., Adil M., Akhtar N., Ashfaq M.Z., Alharthi F.A, & Kazerooni E.A. (2022). Fabrication of Novel Agrowaste (Banana and Potato Peels)-Based Biochar/TiO2 Nanocomposite for Adsorption of Cr(VI), Statistical Optimization via RSM Approach. Polymers, 14(13), 2644.

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Monitoring Polysaccharide Degradation in PEM

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To monitor the progress of degradation, FITC-Chi was used during the assembling process. The as-prepared PEM were incubated in PBS (2 mL, pH 7.2) containing lysozyme (0.1 mg mL⁻¹) at 37 °C with gentle shaking. The concentration of FITC-Chi degraded from LbL films was detected by an ultraviolet spectrophotometer (UV -2250, Shimadzu™, Japan) at 488 nm. The degradation curve was generated according to Eq. 1. All the experiments were performed in triplicate.

R_n = Remaining percentage of FITC-Chi on the surfaces of PEM
W = Gross mass of FITC-Chi in the PEM, μg
n = Designed time point
= Degradation mass

Wang Z., Dong L., Han L., Wang K., Lu X., Fang L., Qu S, & Chan C.W. (2016). Self-assembled Biodegradable Nanoparticles and Polysaccharides as Biomimetic ECM Nanostructures for the Synergistic effect of RGD and BMP-2 on Bone Formation. Scientific Reports, 6, 25090.

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Comprehensive Electrochemical Characterization of Novel Materials

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The
current study used various electrochemical characterization techniques
to analyze the manufactured samples. Transmission electron microscopy
(TEM) (EOL-2100FS, Oxford Xmax 80T with mapping) and scanning electron
microscopy (SEM) (SU8010, Japan) were used to determine the electron
micrograph image morphology of the materials. X-ray diffraction (XRD)
was used to measure the crystal structure of the samples by using
a Bruker D8 diffractometer. BaSO₄ was used as a reference
material for UV–visible diffuse reflectance spectroscopy (DRS)
(via Shimadzu UV-2250 and spectrofluorometer photometer) to determine
photoluminescence (PL) and fluorescence spectra (FS) at a wavelength
of 390 nm. Fourier transform infrared spectroscopy (FT-IR) (PerkinElmer
Spectrum GX, Bruker Vertex 70) was used for elemental analysis. Thermogravimetric
analysis (TGA) was performed to evaluate differential thermal analysis.
Additionally, Brunauer–Emmett–Teller (BET) (MIKE ASAP2460,
2020) was used to analyze the surface area and pore size distribution
of the samples.^{27 (link)}

Sadiq S., Khan I., Humayun M., Wu P., Khan A., Khan S., Khan A., Khan S., Alanazi A.F, & Bououdina M. (2023). Synthesis of Metal–Organic Framework-Based ZIF-8@ZIF-67 Nanocomposites for Antibiotic Decomposition and Antibacterial Activities. ACS Omega, 8(51), 49244-49258.

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Photocatalytic Discoloration of Methylene Blue

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The photocatalytic discoloration of MB dyes was performed on a reformative XPA-7 photocatalytic reaction instrument(Xujiang Electromechanical Plant, Nanjing, China). The incident light power was 162 mW/cm², which was measured by a handheld Optical Power Meter (Newport 1916-R, Newport Corporation, California, CA, USA). The light exposure area of the quartz bottle was about 19.1 cm². The discoloration effect was measured using the absorption spectroscopic technique. In the typical process, an aqueous solution of the MB dyes (10.0 mg/L and 30.0 mL) and 20.0 mg of the as-prepared photocatalysts were mixed in a 50 mL cylindrical quartz tube and left overnight in darkness to reach the adsorption equilibrium for the MB dyes. Then, the mixture was exposed to 1000 W Xe lamp irradiation with or without the light cutoff filters (λ > 420 nm), under ambient conditions and magnetic stirring. At given time intervals, the reaction solution was sampled and analyzed by a UV-visible spectrophotometer (UV 2250, Shimadzu, SHIMADZU (CHINA) Co., Ltd., Shanghai, China).

Liu Y., Chen P., Fan Y., Fan Y., Shi X., Cui G, & Tang B. (2020). Grey Rutile TiO2 with Long-Term Photocatalytic Activity Synthesized Via Two-Step Calcination. Nanomaterials, 10(5), 920.

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Morphological and Spectral Analysis of BI-based Nanoparticles

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The morphology of pure BI and BI-ES-FeAlg/DOX were characterized by TEM (TF20, JEOL 2100F) and SEM (Apreo 2, FEI). The particle size and zeta potential were analyzed by Nano laser particle size analyzer (Zetasizer Nano ZS-90, Malvern). The spectral characteristics were examined by UV-visible spectrophotometer (UV-2250, Shimadzu).

Zhang H., Wang Y., Li M., Cao K., Qi Z., Zhu L., Zhang Z, & Hou L. (2022). A self-guidance biological hybrid drug delivery system driven by anaerobes to inhibit the proliferation and metastasis of colon cancer. Asian Journal of Pharmaceutical Sciences, 17(6), 892-907.

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