Ckx41

Manufactured by Olympus

Sourced in Japan, United States, Germany, Canada, United Kingdom, France, China, Azerbaijan, Italy, Poland, Belgium

The CKX41 is an inverted biological microscope designed for routine observation and documentation of cell cultures and other biological specimens. It features a compact, ergonomic design and supports observation using phase contrast and brightfield illumination modes.

Automatically generated - may contain errors

Lab products found in correlation

Matrigel, by BD (57 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (27 mentions) Crystal violet, by Merck Group (16 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (11 mentions) Matrigel, by Corning (10 mentions) Transwell chamber, by Corning (10 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (9 mentions) Dexamethasone (dex), by Merck Group (8 mentions) U rflt50, by Olympus (8 mentions) Crystal violet, by Beyotime (8 mentions) Sc30 camera, by Olympus (8 mentions) Image pro plus 6, by Media Cybernetics (8 mentions) Penicillin, by Thermo Fisher Scientific (8 mentions) Streptomycin, by Thermo Fisher Scientific (8 mentions) Cellsense entry software, by Olympus (7 mentions) Senescence β galactosidase staining kit, by Cell Signaling Technology (7 mentions) Synergy ht, by Agilent Technologies (7 mentions) Transwell insert, by Corning (7 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions) Ebm 2, by Lonza (6 mentions)

1 569 protocols using ckx41

Wound Healing and Transwell Assays under Fluid Shear Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

We performed wound healing and Transwell assays. For the wound-healing assay, cells were seeded at a density of 10⁵ cells/mL on the slides until 95% confluence. We created wounds created by scraping the cells off of the slides with a plastic scraper. After exposure to 1 dyn/cm² FSS with or without autophagy inhibition treatment (3-MA or lentivirus-derived Atg5 shRNA) for 12 h, we imaged the cells by an invert contrast microscopy (CKX41, Olympus, Japan) and digitized them using a digital camera (G11, Cannon, Japan). The wound healing areas were calculated to evaluate the cell migration capacity by using ImageJ 1.50b.
For Transwell assays, after autophagy inhibition and FSS application for 12 h, we collected the cells and seeded them into the upper chamber of the Transwell assay (10⁶ cells/mL, 8 μm pore membranes, 3422, Corning, USA). For migration, the membrane was lacks matrigel. For invasion, the membrane ws precoated with 5 mg/L matrigel (354230, BD Biosicences, USA) to simulate the extracellular matrix. We added a serum-free medium in the upper chamber and 10% FBS medium in the lower chamber. After 24 h, we stained the migrated or invaded cells on the bottom surface of the membranes with crystal violet, photographed them by an invert contrast microscopy (CKX41, Olympus, Japan) and digitized them using a digital camera (G11, Cannon, Japan), and counted them using ImageJ 1.50b.

Yan Z., Guo D., Tao R., Yu X., Zhang J., He Y., Zhang J., Li J., Zhang S, & Guo W. (2022). Fluid shear stress induces cell migration via RhoA-YAP1-autophagy pathway in liver cancer stem cells. Cell Adhesion & Migration, 16(1), 94-106.

+ Open protocol

+ Expand

Microscopy Analysis of Mitotic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Living native cells were examined with an inverted widefield microscope (Olympus CKX41, Tokyo, Japan). Software Cell^B (Olympus) was used for the acquisition of microscopic images. Fixed, DAPI-labeled cells were viewed under both the Olympus CKX41 and a laser-scanning confocal microscope (Zeiss LSM700, Oberkochen, Germany) equipped with the ZEN 2012 blue edition software (Zeiss). Operators performing the quantification of DAPI-labeled mitotic cells were blinded to the treatment group. Positive cells were counted using the Cell^B software (Olympus) counting tool.

Thole T.M., Lodrini M., Fabian J., Wuenschel J., Pfeil S., Hielscher T., Kopp-Schneider A., Heinicke U., Fulda S., Witt O., Eggert A., Fischer M, & Deubzer H.E. (2017). Neuroblastoma cells depend on HDAC11 for mitotic cell cycle progression and survival. Cell Death & Disease, 8(3), e2635-.

+ Open protocol

+ Expand

3D-printed Patch Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 3D-printed patches with a size of 25 × 25 mm were supplied by the Korea Institute of Science and Technology and Vision Technology Korea. The 3D patches were cut using a biopsy punch with a diameter of 4 mm, and the number of pockets in each patch was observed under a light microscope (CKX41, Olympus, Tokyo, Japan) at ×40 magnification. The swelling ability was performed with 1× PBS, and photographs of the pockets were captured using a light microscope (CKX41, Olympus) at ×100 magnification and Infinity analyze software (Lumenera Corporation, Ottawa, Canada). The pocket size was analyzed using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

Jeon H.R., Kang J.I., Bhang S.H., Park K.M, & Kim D.I. (2024). Transplantation of Stem Cell Spheroid-Laden 3-Dimensional Patches with Bioadhesives for the Treatment of Myocardial Infarction. Biomaterials Research, 28, 0007.

+ Open protocol

+ Expand

Evaluating Cell Invasion Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell invasion ability was observed by spheroid collagen type I invasion assay and tested by Transwell invasion assay based on published protocol [29, (link)30] (link). For spheroid collagen type I invasion assay, HUVECs at a total number of 5.0 × 10 4 in 100 μL EGM-2 medium (Lonza) were seeded on 96-well ultra-low attachment surface plates (Corning) for 2 days to generate cell spheres. Collagen type I (C8062, Solarbio) solution with a final concentration of 1 mg/mL was prepared according to the manufacturer's instructions. The spheres were then embedded in the collagen gel, and 100 μL complete ECM with or without additional VEGF at 50 ng/ mL was added. Invasion of spheres was observed the next day under a microscope (CKX41, Olympus) with a CCD camera (DP70, Olympus). For Transwell invasion assay, the upper chamber of Transwell chamber (Corning) was precoated with 100 μL Matrigel Basement Membrane Matrix (matrigel) (1:20, BD Biosciences) overnight at 37 °C and then cultivated with HUVECs at a total number of 1.0 × 10 5 in 200 μl ECM containing 0.5% FBS without ECGS. 500 μL complete ECM with 50 ng/ml VEGF-165 (Sino Biological) was added to the lower chamber. Cells were cultured for 12 h and were fixed by 4% PFA and stained with crystal violet. Number of cells invading to the lower chamber was observed under a microscope (CKX41; Olympus) with a CCD camera (DP70; Olympus).

Sun J.X., Chang T.F., Li M.H., Sun L.J., Yan X.C., Yang Z.Y., Liu Y., Xu W.Q., Lv Y., Su J.B., Liang L., Han H., Dou G.R, & Wang Y.S. (2018). SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization. Angiogenesis, 21(3).

+ Open protocol

+ Expand

Spheroid Morphology Microscopic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

We observed the spheroid morphology under a light microscope (CKX41, Olympus) at ×40 magnification after 24 h. Images were captured to confirm the diameter of the spheroids using a light microscope (CKX41, Olympus) at ×100 magnification and Infinity analyze software (Lumenera Corporation), and the diameter was analyzed using ImageJ software.

+ Open protocol

+ Expand

Angiogenesis and Migration Assays

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Capillary tube formation assays were carried out as described by Qiu et al [52] (link). Briefly, HUVEC cells (3×10⁴ cells/well) were cultured on Matrigel in a 96-well plate for 24 hrs with 100 µg/mL of P0-P5, P1C-P5C or blank control. The enclosed capillary networks of tubes were photographed by a microscope (Olympus, CKX41, Japan) and the numbers of capillary tubes formed were counted at different time intervals.
The transwell cell migration assay was performed by using a 24-well chamber (Costar, Cambridge, MA, U.S.A.) as the outer chamber and polycarbonate filters (8 µm pores) as the inner chamber. HUVECs were starved overnight in serum-free F12 medium, harvested by trypsinization and 5×10⁴ cells were seeded into the inner chambers in F12 (1%FBS) media with 100 µg/mL P5 or blank control. The outer chambers contained the F12 media with 10% FBS. After incubation for 18 hrs at 37°C, the cells on the lower surface of the filter were fixed and stained with 0.1% crystal violet. Images of the migrated cells were taken using a microscope (Olympus, CKX41, Japan). The migrated cells were quantified at 595 nm after extraction with 10% acetic acid. Migrated cells in the outer chambers were also quantified independently by incubating the out chambers in F12 media with 10% FBS for 8 days followed by resazurin quantification.

Zeng Y., Han Z., Qiu P., Zhou Z., Tang Y., Zhao Y., Zheng S., Xu C., Zhang X., Yin P., Jiang X., Lu H., Yu G, & Zhang L. (2014). Salinity-Induced Anti-Angiogenesis Activities and Structural Changes of the Polysaccharides from Cultured Cordyceps Militaris. PLoS ONE, 9(9), e103880.

+ Open protocol

+ Expand

Intestinal Tissue Histology and IgA+ Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two centimetres of small intestinal tissue was used for the preparation of 3 μm sections using Senior Rotary Microtome (Radical, RMT-30) (17) . The histological slides were stained with haematoxylin and eosin stain for counting goblet cells and to determine villi morphology using an inverted light microscope (Olympus, CKX41). The unstained histological slides were prepared for direct immunofluorescence assay of IgA + cells (16) using α-chain mono-specific antibody (1:100 dilution) conjugated with fluorescein isothiocyanate (Cayman Chemical) and were observed under a fluorescent light microscope (CKX41; Olympus). The number of fluorescent cells was counted in thirty fields at 200 × magnification. The results are expressed as the number of positive fluorescent cells per ten fields of vision.

Saliganti V., Kapila R., Sharma R, & Kapila S. (2015). Feeding probiotic Lactobacillus rhamnosus (MTCC 5897) fermented milk to suckling mothers alleviates ovalbumin-induced allergic sensitisation in mice offspring. The British journal of nutrition, 114(8).

+ Open protocol

+ Expand

Osteogenic Differentiation of PDL Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

PDL cells were seeded into 24 well plates at a density of 5 × 10⁴ cells/well in the osteogenic differentiation medium. ALP activity and mineralization were estimated on days 3, 5, 7, 14, 21, and 28. To assess ALP activity, the cultured cells were washed twice with PBS and fixed in 4% paraformaldehyde solution at room temperature for 15–30 min. Then, the fixed cells were rinsed three times with deionized water and stained using the ALP staining kit (Wako, Osaka, Japan) according to the manufacturer’s protocols. The cells were reacted with 250 μL ALP staining solution for 40 min in the dark and observed by inverted microscopy (CKX41, Olympus, Tokyo, Japan). For the mineralization assay, ARS solution (Sigma-Aldrich, MO, USA) was adjusted with 0.5% NH₄OH to pH 4.1–4.3, followed by filtration through a 0.2 μm filter. The cells were incubated in the solution for 40–60 min, dried at room temperature, washed with deionized water, and observed by inverted microscopy (CKX41, Olympus).

Kim A., Kim A.R., Jeon Y.E., Yoo Y., Yang Y.M, & Bak E. (2023). TRPC expression in human periodontal ligament cells and the periodontal tissue of periodontitis mice: a preliminary study. Laboratory Animal Research, 39, 19.

+ Open protocol

+ Expand

Cellular Invasion Assay using Transwell System

Check if the same lab product or an alternative is used in the 5 most similar protocols

5% CO 2 for 48 h. The cells in each group were then fixed and observed under a microscope (CKX41; Olympus Corporation, Tokyo, Japan.
Invasion assay. A Transwell assay was conducted for invasion analysis (using Matrigel-coated polyethylene terephthalate membrane chambers; BD Biosciences), and a cell suspension containing 5x10 5 cells/ml was prepared in serum-free DMEM. A total of 300 µl cell suspension was then added into the upper chamber and 500 µl RPMI-1640 (Invitrogen: Thermo Fisher Scientific, Inc.) supplemented with 10% FBS was added into the lower chamber. Following incubation for 24 h, non-invading cells as well as the matrix gel (BD Biosciences) on the interior of the inserts was removed using a cotton-tipped swab. Invasive cells on the lower surface of the membrane were stained using 0.1% crystal violet (Beyotime Institute of Biotechnology) for 20 min, and then rinsed with water and dried. Five fields were randomly selected, and cell number was counted under a microscope (CKX41; Olympus Corporation).

Xue M., Zhao L., Yang F., Li Z, & Li G. (2016). MicroRNA‑145 inhibits the malignant phenotypes of gastric carcinoma cells via downregulation of fascin 1 expression. Molecular medicine reports, 13(1).

+ Open protocol

+ Expand

Evaluating BMSC Migration and Osteogenic Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The co-cultured BMSCs were fixed with 4% paraformaldehyde for 20 min and then stained with crystal violet for 15 min to detect the migration of BMSCs. Areas of interest were selected under the optical microscope (CKX-41, Olympus Corp., Japan).
To evaluate the ALP activity of BMSCs, the co-cultured BMSCs were fixed with 4% paraformaldehyde for 15 min and stained with ALP solution according to the manufacturer's protocol. Imaging was obtained through an optical microscope (CKX-41, Olympus Corp., Japan). To evaluate the deposited minerals, the co-cultured BMSCs were fixed with 4% paraformaldehyde for 15 min and then stained with 1% AR staining solution (Lot. No.20180820, Solarbio, China) to detect the degree of mineralization. Subsequently, AR was further isolated with cetylpyridinium chloride and was detected using a spectrophotometer (iMark, Bio-Rad Laboratories, Inc., USA).

Lu Y., Liu S., Yang P., Kou Y., Li C., Liu H, & Li M. (2022). Exendin-4 and eldecalcitol synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells through M2 macrophages polarization via PI3K/AKT pathway. Stem Cell Research & Therapy, 13, 113.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!