35s sodium sulfate

Manufactured by PerkinElmer

35S-sodium sulfate is a radioactive isotope of sodium sulfate that is commonly used as a tracer in various research applications. It emits beta radiation and has a half-life of approximately 87 days. The product is typically supplied as a solution and can be used to label or track the movement of sulfur-containing compounds in biological systems or other research experiments.

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Lab products found in correlation

Pd 10 column, by Cytiva (2 mentions) Sulfate free dulbecco s modified eagle s medium dmem as31600 cat no 074, by Thermo Fisher Scientific (2 mentions) Superdex peptide 10 300, by Cytiva (2 mentions) Deae sephacel, by Cytiva (2 mentions) Sephadex g 25, by Cytiva (2 mentions) Superose 6 10 300, by Cytiva (2 mentions) Prestoblue cell viability reagent, by Thermo Fisher Scientific (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Recombinant human adiponectin, by BioVendor (1 mentions) Non essential amino acids neaa, by Thermo Fisher Scientific (1 mentions) Bisbenzimide hoechst 33258, by Merck Group (1 mentions) Calf thymus dna, by Merck Group (1 mentions) Proteinase k, by Thermo Fisher Scientific (1 mentions) Ammonium acetate, by Merck Group (1 mentions) L ascorbic acid 2 phosphate, by Merck Group (1 mentions) Proline, by Merck Group (1 mentions) Cytotox one homogeneous membrane integrity assay kit, by Promega (1 mentions) 1 9 dimethylmethylene blue dmmb, by Merck Group (1 mentions) 3h proline, by PerkinElmer (1 mentions) Shark chondroitin sulfate, by Merck Group (1 mentions)

Close spelling variants of this product

35S-sulfate (11 citations) Carrier-free sodium [35S]sulfate (3 citations)

3 protocols using 35s sodium sulfate

Quantitative Assessment of DS-epi1 Levels

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³⁵S-sodium sulfate (1,500 Ci/mmol) was from PerkinElmer. Sulfate-free Dulbecco’s modified Eagle’s medium (DMEM) (AS31600 cat no. 074-91083P) was from Gibco. Superose 6 10/300, Superdex Peptide 10/300, PD-10 columns, Sephadex G-25, DEAE-Sephacel were from Cytiva. DS-epi1 was detected by Western blot with 1 μg/mL of an immunopurified anti-DS-epi1 rabbit polyclonal antibody obtained by immunization with the peptide antigen (KWSKYKHDLAAS, corresponding to amino acids 509 to 520 of the human/murine sequence; Innovagen, Sweden) (Maccarana et al. 2006 (link)). The Biorad stain-free gels were used, and both loading control, i.e. the blotted bands, and the chemioluminiscent signal were visualized with the ChemiDoc Imaging System (Biorad). Cell number was evaluated by DNA staining with crystal violet. Viability was assessed with PrestoBlue™ Cell Viability Reagent (Invitrogen).

Maccarana M., Li B., Li H., Fang J., Yu M, & Li J.P. (2024). Inhibitors of dermatan sulfate epimerase 1 decreased accumulation of glycosaminoglycans in mucopolysaccharidosis type I fibroblasts. Glycobiology, 34(6), cwae025.

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Bovine Chondrocyte Isolation and Culture

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Immature bovine stifles were from Research 87 (Marlborough, MA). High glucose Dulbecco’s modified Eagle’s medium (DMEM) was from HyClone (Logan, UT). Non-essential amino acids (NEAA) and proteinase K were from Life Technologies (Carlsbad, CA). N-(2-hydroxyethyl)-piperazine-N′-2-ethanesulfonic acid (HEPES), gentamicin and phosphate buffered saline (PBS) were from Mediatech (Manassas, VA). Insulin-transferrin-selenous acid premix (ITS+) was from BD Biosciences (Franklin Lakes, NJ). L-ascorbic acid 2-phosphate, proline, ammonium acetate, 1,9-dimethyl-methylene blue (DMMB), shark chondroitin sulfate, sodium nitrite, bisbenzimide (Hoechst 33258), and calf thymus DNA were from Sigma (St. Louis, MO). Sulfanilamide reagent and naphthylethylenediamine dihydrochloride solution were from Ricca Chemical (Arlington, TX). The CytoTox-ONE^™ Homogeneous Membrane Integrity assay kit was from Promega (Madison, WI). ³H-proline and ³⁵S-sodium sulfate were from Perkin Elmer (Waltham, MA). Recombinant human leptin was from Shenandoah Biotechnology (Warwick, PA), recombinant human visfatin was from Novus Biologicals (Littleton, CO), and recombinant human adiponectin and resistin were from Biovendor (Candler, NC).

Nishimuta J.F, & Levenston M.E. (2015). Meniscus is more susceptible than cartilage to catabolic and anti-anabolic effects of adipokines. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, 23(9), 1551-1562.

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Ebselen Sulfate Metabolism Assay

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Ebselen was purchased from Sigma and 50 mM stock solution was prepared in DMSO. ³⁵S-sodium sulfate (1500 Ci/mmol) was from PerkinElmer. Sulfate-free Dulbecco’s modified Eagle’s medium (DMEM) (AS31600 cat no. 074-91083P) was from Gibco. Superose 6 10/300, Superdex Peptide 10/300, PD-10 columns, Sephadex G-25, DEAE-Sephacel were from Cytiva.

Maccarana M., Tykesson E., Pera E.M., Gouignard N., Fang J., Malmström A., Ghiselli G, & Li J.P. (2021). Inhibition of iduronic acid biosynthesis by ebselen reduces glycosaminoglycan accumulation in mucopolysaccharidosis type I fibroblasts. Glycobiology, 31(10), 1319-1329.

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