Log In Sign up
The largest database of trusted experimental protocols

Chemiluminescent film

Manufactured by Thomas Scientific
Visit Supplier

Chemiluminescent film is a type of photographic film used for the detection and visualization of chemiluminescent signals. It is designed to capture and record light emission from chemiluminescent reactions, which occur when certain chemicals react and release energy in the form of light.

Automatically generated - may contain errors

Lab products found in correlation

Radioimmunoprecipitation assay buffer, by Thermo Fisher Scientific (2 mentions) Mini protean tgx gel, by Bio-Rad (2 mentions) Pser73 jun, by Cell Signaling Technology (2 mentions) C jun, by Cell Signaling Technology (2 mentions) Horseradish peroxidase conjugated anti rabbit igg secondary antibody, by Santa Cruz Biotechnology (2 mentions) Clarity western ecl, by Bio-Rad (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Protease and phosphatase inhibitor, by Roche (2 mentions)

2 protocols using chemiluminescent film

1

Quantifying Protein Expression After Transfection or Irradiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines at various times post-transfection or after γ-irradiation were collected and lysed in radioimmunoprecipitation assay buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitors (Roche) as previously described36 (link). 20μg protein was resolved onto 4-20% mini-PROTEAN TGX gels (Bio-Rad), and transferred to PVDF membranes. Primary antibodies were used to detect pSer73-JUN (1:1000, Cell Signaling Technology, #3270), c-JUN (1:1000, Cell Signaling Technology, #9165), and β-actin (1:1000, Cell Signaling Technology, #4970). Following incubation with anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:10,000, Santa Cruz, #2030), expression was detected via Clarity Western ECL (BioRad) and chemiluminescent film (Denville Scientific). Band intensities were quantified using NIH Image J software.
Metheetrairut C., Adams B.D., Nallur S., Weidhaas J.B, & Slack F.J. (2016). cel-mir-237 and its homologue, hsa-miR-125b, modulate the cellular response to ionizing radiation. Oncogene, 36(4), 512-524.
+ Open protocol
+ Expand
2

Quantifying Protein Expression After Transfection or Irradiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines at various times post-transfection or after γ-irradiation were collected and lysed in radioimmunoprecipitation assay buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitors (Roche) as previously described36 (link). 20μg protein was resolved onto 4-20% mini-PROTEAN TGX gels (Bio-Rad), and transferred to PVDF membranes. Primary antibodies were used to detect pSer73-JUN (1:1000, Cell Signaling Technology, #3270), c-JUN (1:1000, Cell Signaling Technology, #9165), and β-actin (1:1000, Cell Signaling Technology, #4970). Following incubation with anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:10,000, Santa Cruz, #2030), expression was detected via Clarity Western ECL (BioRad) and chemiluminescent film (Denville Scientific). Band intensities were quantified using NIH Image J software.
Metheetrairut C., Adams B.D., Nallur S., Weidhaas J.B, & Slack F.J. (2016). cel-mir-237 and its homologue, hsa-miR-125b, modulate the cellular response to ionizing radiation. Oncogene, 36(4), 512-524.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!