The extracted proteins were separated by 10% SDS-PAGE and further transferred onto PVDF membranes (Millipore, Bedford, MA, United States). Antibodies including NAP1L1 (Abcam), CCND1 (Abcam), HDGF (Proteintech), and c-Jun (Proteintech) were used in the Western blot assays following the manufacturer’s instructions. Detection was performed using ECL Plus Western blotting detection reagents (Millipore, United States). The specific protein expression levels of the blots were normalized to GAPDH (Santa). The Cat numbers, origins, and dilution concentrations used for all antibodies are listed in Supplementary Table 2 .
Nap1l1
Manufactured by Abcam
Sourced in United Kingdom
NAP1L1 is a histone chaperone that facilitates nucleosome assembly. It is involved in the regulation of chromatin structure and gene expression.
Automatically generated - may contain errors
2 protocols using nap1l1
1
Western Blot Protein Quantification
2
Protein Expression Analysis by Western Blotting
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Western blot analysis was performed as previously reported
[20] (link). Briefly, the extracted proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, USA). Antibodies against NAP1L1 (Abcam, Cambridge, UK), CCND1 (Abcam), HDGF (Proteintech, Rosemont, USA), DDX5 (Abcam), β-catenin (CST, Beverly, USA), and GAPDH (Santa Cruz Biotech, Santa Cruz, USA) were used as the primary antibodies. Detection was performed using ECL Plus Western blotting detection reagents (Millipore). The specific protein expression levels of the blots were normalized to that of GAPDH. The information of the antibodies used in this study is listed in
Supplementary Table S3 .
[20] (link). Briefly, the extracted proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, USA). Antibodies against NAP1L1 (Abcam, Cambridge, UK), CCND1 (Abcam), HDGF (Proteintech, Rosemont, USA), DDX5 (Abcam), β-catenin (CST, Beverly, USA), and GAPDH (Santa Cruz Biotech, Santa Cruz, USA) were used as the primary antibodies. Detection was performed using ECL Plus Western blotting detection reagents (Millipore). The specific protein expression levels of the blots were normalized to that of GAPDH. The information of the antibodies used in this study is listed in
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