Cd25 fitc

Manufactured by BD

Sourced in United States

CD25-FITC is a fluorescent-labeled antibody specific for the human CD25 (IL-2Rα) antigen. CD25 is a type I transmembrane protein that is expressed on activated T cells, B cells, and monocytes. This product can be used for the detection and enumeration of CD25-positive cells by flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Cd4 pe, by BD (10 mentions) Cd4 percp, by BD (7 mentions) Cd127 pe, by BD (5 mentions) Foxp3 apc, by Thermo Fisher Scientific (5 mentions) Foxp3 pe, by BD (5 mentions) Facscalibur, by BD (5 mentions) Cd45 fitc, by BD (4 mentions) Cd4 apc cy7, by BD (4 mentions) Foxp3 pe, by Thermo Fisher Scientific (4 mentions) Cd3 apc, by BD (4 mentions) Cd8 fitc, by BD (3 mentions) Cd3 apc cy7, by BD (3 mentions) Cd8 apc cy7, by BD (3 mentions) Cd4 apc, by BD (3 mentions) Lsrfortessa, by BD (3 mentions) Cd45 apc, by BD (3 mentions) Cd11c percp cy5, by BD (3 mentions) Cd3 fitc, by BD (3 mentions) Rorγt pe, by Thermo Fisher Scientific (3 mentions) Facscanto 2, by BD (3 mentions)

Close spelling variants of this product

FITC-labeled anti-CD25 (2 citations) Anti-CD25-FITC (29 citations) Fluorochrome-conjugated monoclonal antibodies specific for human CD4–FITC and CD25-PE (2 citations) Anti-CD25-FITC (clone M-A251), (3 citations) FITC-conjugated anti-CD25 (clone 7d4), (2 citations) Anti-CD25-FITC (clone 7D4, (2 citations) Anti-mouse CD25-FITC (4 citations) FITC-conjugated anti-CD25 antibody (2 citations) Mouse anti-human CD4 FITC and anti-human CD25 FITC (2 citations) FITC-conjugated anti-mouse CD25 (2 citations)

46 protocols using cd25 fitc

Evaluating MSC Induction of Tregs

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the capacity of MSCs to induce the generation of T-regulatory (Treg) cells, PBMC-MSC cocultures have been set up. In short, 500 × 10³ PBMCs from five different donors obtained by Ficoll gradient centrifugation were plated in the presence of 50,000 allogeneic MSCs in RPMI 10% FBS with IL-2300 U/ml in a 24-multiwell flat-bottomed plate for 1 week of culture [36 (link)]. At day 7, PBMCs were harvested and analyzed by flow cytometry after incubation with the following conjugated monoclonal antibodies: CD25 FITC, CD127 PE, CD4 APC, CD3 PECy7 and CD45 APCCy7 (Becton Dickinson). Treg cell induction by MSCs was evaluated as the percentage of CD25^High/CD4⁺/CD127^Low/− PBMCs [37 (link)].

Becherucci V., Piccini L., Casamassima S., Bisin S., Gori V., Gentile F., Ceccantini R., De Rienzo E., Bindi B., Pavan P., Cunial V., Allegro E., Ermini S., Brugnolo F., Astori G, & Bambi F. (2018). Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience. Stem Cell Research & Therapy, 9, 124.

+ Open protocol

+ Expand

Flow Cytometry Profiling of Lymphocyte Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry analysis was performed to quantify lymphocyte subsets on BD LSR II flow cytometer with BD FACS DIVA software (BD Biosciences, Heidelberg, Germany). The following antibodies were used for phenotypic analysis: CD45-FITC, CD19/20-PE, CD14-Cy-7, CD25-FITC, CD127-PE, CD45RA-APC, CD3-APC Cy-7, CD8-FITC, CD4-PE, CD4-APC Cy7, CD8-APC Cy7, CD62L-FITC, CCR7-PE, CD27-FITC, CD62L-PE, (Beckton Dickinson, Franklin Lakes, NJ, USA), CD3-ECD, CD56-APC, CD45RO-ECD (Beckman Coulter, Miami, FL, USA).^{30 (link)}

Triplett B.M., Shook D.R., Eldridge P., Li Y., Kang G., Dallas M., Hartford C., Srinivasan A., Chan W.K., Suwannasaen D., Inaba H., Pui C.H, & Leung W. (2015). Rapid Memory T-cell Reconstitution Recapitulating CD45RA-depleted Haploidentical Transplant Graft Content in Patients with Hematologic Malignancies. Bone marrow transplantation, 50(7), 968-977.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD4-PE, CD25-FITC, CD127-APC, FITC-lineage-cocktail (CD3, CD14, CD16, CD19, CD20, CD56), HLA-DR-PerCP, CD123-PE, CD11c-APC, CD3-PE-Cy5, CD4-FITC, CD8-PE, mouse isotype controls, and lysis solution were purchased from Becton Dickinson (Franklin Lakes, NJ, USA).

Qi W., Ren Y., Fu R., Wang H., Liu C, & Shao Z. (2015). Detection and Significance of CD4+CD25+CD127dim Regulatory T Cells in Individuals with Severe Aplastic Anemia. Turkish Journal of Hematology, 32(3), 220-227.

+ Open protocol

+ Expand

Flow Cytometry Profiling of Lymphocyte Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Comprehensive Immune Phenotyping of PBMC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immune activation and differentiation were quantified as previously described [54] (link). In brief, one million thawed PBMC were stained with either an activation or differentiation panel for 15 minutes at 37°C prior to fixation in formaldehyde. Both panels included CD3 V450 (Becton Dickinson); CD4 PE-Texas Red (Invitrogen); CD8 Qdot605 (Invitrogen). Activation panel included HLA-DR FITC; PD-1 AF647; CD38 PE; CCR5 PE-Cy5; 45RA PE-Cy7 (all Becton Dickinson); CCR7 APC eFluor-780 (eBioscience). Differentiation panel included CD45RA PE; CD28 PE-Cy5; CCR7 PE-Cy7; CD31 FITC (all Becton Dickinson); CD57 AF647 (Biolegend); CD27 AF780 (eBioscience). For Tregs, PBMC were surface stained with CD4 PerCP, CD127 PE and CD25 FITC (all Becton Dickinson) followed by intracellular staining using eBioscience FoxP3 staining kit and FoxP3 APC as per manufacturer's instructions. Data was acquired on a BD LSR-Fortessa and analysed using FlowJo version 10.

Elliott J.H., Wightman F., Solomon A., Ghneim K., Ahlers J., Cameron M.J., Smith M.Z., Spelman T., McMahon J., Velayudham P., Brown G., Roney J., Watson J., Prince M.H., Hoy J.F., Chomont N., Fromentin R., Procopio F.A., Zeidan J., Palmer S., Odevall L., Johnstone R.W., Martin B.P., Sinclair E., Deeks S.G., Hazuda D.J., Cameron P.U., Sékaly R.P, & Lewin S.R. (2014). Activation of HIV Transcription with Short-Course Vorinostat in HIV-Infected Patients on Suppressive Antiretroviral Therapy. PLoS Pathogens, 10(11), e1004473.

+ Open protocol

+ Expand

Immune Cell Characterization by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The procedure used in this study has been published previously (14 (link),26 (link)). Briefly, the following anti-mouse monoclonal antibodies were purchased from BD Biosciences: Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 (1A8; 560602), Ly6C-fluorescein isothiocyanate (FITC) (AL-21; 553104), CD4-allophycocyanin (APC) (RM4-5; 553051), CD25-FITC (7D4; 553071), and CD19-APC (1D3; 550992). The following antibodies were purchased from BioLegend: CD45-FITC (30F-11;103108), CD45-PerCP-Cy5.5 (30F-11;103132), CD45-brilliant violet (BV510) (30F-11;103138), CD11b-APC/Fire750 (M1/70;101262), CD244-Alexa Fluor 647 (2B4;133509), F4/80-phycoerythrin (PE) (BM8;123110), PD-L1-BV421 (10F.9G2;124315), CD8a-APC/Fire 750 (53-6.7;100766), CD8a-PE (53-6.7; 100708), CD127-BV510 (A7R34;135033), CD1d-PE (1B1;123509), and CD5-BV421 (53-7.3;100618). The isolated immune cells were stained in a 96-round-bottom-well plate for 20 min at 4°C and washed with PBS containing 1% bovine serum albumin. Sorting of Ly6G⁺CD244⁺ and Ly6G⁺CD244⁻ cells was conducted on a FACSAria instrument (BD Biosciences). Flow cytometric data were obtained by a FACS Verse instrument (BD Biosciences) and analyzed by FlowJo software (TreeStar, Inc.). CD45-gated cells were analyzed in this study.

Sugita Y., Yamashita K., Fujita M., Saito M., Yamada K., Agawa K., Watanabe A., Fukuoka E., Hasegawa H., Kanaji S., Oshikiri T., Matsuda T., Nakamura T., Suzuki S, & Kakeji Y. (2021). CD244+ polymorphonuclear myeloid-derived suppressor cells reflect the status of peritoneal dissemination in a colon cancer mouse model. Oncology Reports, 45(6), 106.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The presence of CD14, CD25, CD28, CD45, CD61, CD90, TLR1 and TLR4 were analyzed using a flow cytometer (CYTOMICS FC 500, Beckman Coulter, Miami, FL) according to manufacturer's instruction. Anti-CD14-PE-Cy7, CD25-FITC, TLR4-PE, CD28-PE, CD61-PE, CD90-FITC and CD45-APC were purchased from (BD Biosciences) ; anti-TLR1-PE was purchased from (eBioscience).

Ding S.M., Lu J.F., Edoo M.I., Zhou L., Xie H.Y., Zheng S.S, & Li Q.Y. (2019). MRC-5 Cancer-associated Fibroblasts Influence Production of Cancer Stem Cell Markers and Inflammation-associated Cell Surface Molecules, in Liver Cancer Cell Lines. International Journal of Medical Sciences, 16(8), 1157-1170.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells from the bone marrow, thymus, spleen, liver, and peripheral blood were stained and analyzed on a FACSCanto (BD Biosciences) running FACSDiva (v.6.1.3) and FlowJo (v.7.6.1, FlowJo LLC) software. Lymphocytes were identified and gated based on their forward-scattered light (FSC) and side-scattered light (SSC) profiles. Cell suspensions were treated with ACK lysis buffer (150 mmol/L NH₄Cl, 10 mmol/L KHCO₃, and 0.1 mmol/L disodium EDTA) and then stained with the following conjugated antibodies: B220-APC-Cy7 (BD Biosciences), CD2-FITC (BD Biosciences), CD3ε-PE (BD Biosciences), CD3ε-Alexaflore 647 (BD Biosciences), CD4-FITC (Caltag Laboratories), CD8a-APC (Caltag Laboratories), CD8a-Alexaflore 647 (BD Biosciences), CD25-FITC (BD Biosciences), CD44-PE (BD Biosciences), CD45-APC (BD Biosciences), Ki-67-FITC (BD Biosciences), Ly5.1-PerCP-Cy5.5 (BD Biosciences), Ly5.2-PerCP-Cy5.5 (BD Biosciences), NK1.1-APC (Caltag Laboratories), and Sca1-Alexaflore 647 (BD Biosciences).

Ginn S.L., Hallwirth C.V., Liao S.H., Teber E.T., Arthur J.W., Wu J., Lee H.C., Tay S.S., Hu M., Reddel R.R., McCormack M.P., Thrasher A.J., Cavazzana M., Alexander S.I, & Alexander I.E. (2016). Limiting Thymic Precursor Supply Increases the Risk of Lymphoid Malignancy in Murine X-Linked Severe Combined Immunodeficiency. Molecular Therapy. Nucleic Acids, 6, 1-14.

+ Open protocol

+ Expand

Mesenchymal Stem Cell Surface Antigen Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cellular differentiation analysis was performed using flow cytometry. To analyze the cell surface antigen expressions, the cells after third passage was used. MSCs were incubated with antibodies for rat CD90 FITC (BD Biosciences, San Diego, CA, USA), CD 29 FITC (BD Biosciences, San Diego, CA, USA), CD106 PE (BD Biosciences, San Diego, CA, USA), CD54 PE (BD Biosciences, San Diego, CA, USA) at room temperature in the dark. Control antibodies were FITC Rat IgG2a, K isotype controls and IgG1 PE isotype controls (BD Biosciences, San Diego, CA, USA). Negative markers were CD3 PE (BD Biosciences, San Diego, CA, USA), CD4 APC (BD Biosciences, San Diego, CA, USA), CD25 FITC (BD Biosciences, San Diego, CA, USA), CD45 FITC (BD Biosciences, San Diego, CA, USA), CD8B FITC (BD Biosciences, San Diego, CA, USA).

Çerman E., Akkoç T., Eraslan M., Şahin Ö., Özkara S., Vardar Aker F., Subaşı C., Karaöz E, & Akkoç T. (2016). Retinal Electrophysiological Effects of Intravitreal Bone Marrow Derived Mesenchymal Stem Cells in Streptozotocin Induced Diabetic Rats. PLoS ONE, 11(6), e0156495.

+ Open protocol

+ Expand

Characterization of Activated T Cells in RA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Previously frozen PBMCs from RA subjects were cultured at 5 × 10⁶ total cells/well within a 24 well plate in RPMI-1640 + 10% pooled human serum with 10 µg/ml of peptide. IL-2 (Novartis) was added at 325 IU/ml on day 6. After 14 days cells were stained for expression of CD25 FITC (BD), CD4 PerCP (BioLegend), Annexin V APC (BD), CD14 APC (BioLegend), CD19 APC (BioLegend), and tetramer before being run on a FACSCalibur. The data was analyzed by FlowJo software version 9.6.2 (Tree star).

James E., Rieck M., Pieper J., Gebe J.A., Yue B.B., Tatum M., Peda M., Sandin C., Klareskog L., Malmström V, & Buckner J.H. (2014). Citrulline specific Th1 cells are increased in rheumatoid arthritis and their frequency is influenced by disease duration and therapy. Arthritis & rheumatology (Hoboken, N.J.), 66(7), 1712-1722.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!