Ccr4 bv421

Manufactured by BioLegend

The CCR4 BV421 is a flow cytometry reagent that detects the expression of the CCR4 chemokine receptor on the surface of cells. It can be used to identify and characterize CCR4-positive cell populations.

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2 protocols using ccr4 bv421

Comprehensive CD4+ T-cell Characterization

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CD4 T‐cell purity, composition and transfection efficiency were assessed by flow cytometry (BD LSR II). Purity and composition panel (stained in 250 μl): CD4 APC (#357408), CCR4 BV421 (#359414), CCR6 AF700 (#353434), CD3 FITC (#300306), CD62L PerCP/Cy5.5 (#304824), CXCR3 PE/Dazzle 594 (#353736) and CD45RA PE/Cy7 (#304126)—all used at 1:100 dilution (all BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend) and Fc receptor blocking reagent (Miltenyi). Transfection efficiency panel: EGFP, CD4 APC (as above), Zombie Aqua Fixable Viability Kit, Fc receptor blocking reagent. Data were gated using FlowJo v10 (BD).

Bourges C., Groff A.F., Burren O.S., Gerhardinger C., Mattioli K., Hutchinson A., Hu T., Anand T., Epping M.W., Wallace C., Smith K.G., Rinn J.L, & Lee J.C. (2020). Resolving mechanisms of immune‐mediated disease in primary CD4 T cells. EMBO Molecular Medicine, 12(5), e12112.

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Multiparameter Flow Cytometry Analysis

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The following fluorochrome-conjugated anti-human mAb were used for flow cytometry studies: LAG3 BV650, TIGIT PE-Cy7, CTLA4 PE, CD25 PE-Dazzle 594, TIM3 PerCP-Cy5.5, CD19 Alexa Fluor 700, CCR4 BV421, ICOS PE-Cy7, CCR5 PE-Dazzle 594, HLA-DR PE, CCR7 FITC, CD38 BV711, PD-L1 BV711, CCR6 Alexa Fluor 700, PD-1 BV421, CD40L BV605, perforin PE-Dazzle 594, and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD4 APC-H7, CD127 BV605, Ki-67 PerCP-Cy5.5, PD-1 BV650, CXCR3 BV605, CD69 BV650, IL-2 BV711, CXCR5 Alexa Fluor 647, IFN-γ PE-Cy7, TNF-α FITC, granzyme B Alexa Fluor 700 and CD27 BV480 from BD Biosciences (San Jose, CA); IL-21 PE from eBioscience, (San Diego, CA); and CD45RO PE-Cy5.5 from Beckman Coulter (Fullerton, CA). LIVE/DEAD Fixable Blue Dead Cell Stain Kit from Thermo Fisher Scientific (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated for optimum concentration before usage.

Rinaldi S., de Armas L., Dominguez-Rodríguez S., Pallikkuth S., Dinh V., Pan L., Gӓrtner K., Pahwa R., Cotugno N., Rojo P., Nastouli E., Klein N., Foster C., De Rossi A., Giaquinto C., Rossi P., Palma P, & Pahwa S. (2021). T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals. PLoS Pathogens, 17(4), e1009533.

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