The House Ear Institute-Organ of Corti 1 (HEI-OC1) cell line is extremely sensitive to ototoxic drugs and expresses specific markers that are characteristic of organ of Corti cells. Therefore, this cell line has been used as an in vitro system to investigate the mechanisms underlying ototoxicity and to screen new drugs for otoprotective properties [29 (link)]. HEI-OC1 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (GIBCO BRL, Grand Island, NY, USA) with 10% fetal bovine serum (Lonza, Walkersville, MD, USA). Cells were allowed to continuously proliferate without differentiation in a 5% CO2 environment at 33 °C. The cells were exposed to cisplatin or Tempol (Sigma, St. Louis, MO, USA) for 24 h in culture, and the Tempol pre-treatment group was pre-treated with Tempol for 2 h and then exposed to cisplatin for 24 h in culture.
Tempol
Manufactured by Merck Group
Sourced in United States, Germany, Italy, Brazil, Japan, Spain, Switzerland, France
Tempol is a laboratory product developed by Merck Group. It is a nitroxide compound that functions as a stable free radical. Tempol is commonly used as a spin label and spin trap in various scientific research applications.
Automatically generated - may contain errors
Lab products found in correlation
Apocynin, by Merck Group (17 mentions)
N acetyl cysteine (nac), by Merck Group (8 mentions)
Indomethacin, by Merck Group (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (6 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (5 mentions)
Lucigenin, by Merck Group (5 mentions)
Antimycin a, by Merck Group (4 mentions)
Lipopolysaccharides (lps), by Merck Group (4 mentions)
U46619, by Merck Group (4 mentions)
L name, by Merck Group (4 mentions)
Capsaicin, by Merck Group (4 mentions)
Mitotracker red, by Thermo Fisher Scientific (4 mentions)
Epigallocatechin gallate (egcg), by Merck Group (3 mentions)
Mitosox, by Thermo Fisher Scientific (3 mentions)
Co2 incubator, by Thermo Fisher Scientific (3 mentions)
N acetyl l cysteine, by Merck Group (3 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (3 mentions)
L nna, by Merck Group (3 mentions)
Nadph, by Merck Group (3 mentions)
Ang 2, by Merck Group (3 mentions)
145 protocols using tempol
1
Evaluating Ototoxicity and Otoprotection in HEI-OC1 Cells
2
Triclosan-Induced Oxidative Stress and STAT3 Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Triclosan was obtained from Shanghai Baidi Biody-Bio Co., Ltd. Hoechst, Cal-AM, Eth-1, Fluo-3/AM, mito-Tracker, mito-SOX, tetramethylrhodamine methyl ester (TMRM) and DAPI were purchased from Thermo Fisher Scientific, Inc. Dihydroethidium dye was purchased from the Beyotime Institute of Biotechnology. Tempol, 3-MA and acetylcysteine (NAC) were purchased from Sigma Aldrich; Merck KGaA. Tempol (0.5 and 1 mM) is a radical scavenger that was used to test the effect of ROS levels on cytotoxicity induced by Triclosan (20 µM) in the lactate dehydrogenase (LDH) release assay. S3I-201 was purchased from EMD Millipore. S3I-201 (10 and 20 µM) is a STAT3 inhibitor that was used to detect the effect of STAT3 activity change on cytotoxicity induced by Triclosan (20 µM) in the LDH release assay. Anti-p-STAT3 (Y705, #9131, 1:1,000), anti-STAT3 (#4904, 1:1,000), anti-p-AMPK (Thr172, #2535, 1:1,000), anti-AMPK (#2532, 1:1,000) and anti-p62 (#88588, 1:1,000) were purchased from Cell Signaling Technology, Inc., Bcl-2 (ab196495, 1:1,000) antibody was purchased from Abcam and LC3 (L7543, 1:1,000) antibody was purchased from Sigma Aldrich; Merck KGaA.
3
Mesenteric Artery Contractility Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rings of second-order mesenteric arteries were isolated to measure contractile responses. Mesenteric arteries were mounted in a Mulvany-style small vessel myograph (Danish Myo Technology, Denmark) containing Krebs-bicarbonate buffer (in mmol/L: D-glucose 11.1, CaCl2 2.5, NaCl 118, KCl 4.5, KH2PO4 1.03, MgSO4 0.45, NaHCO3 25) and bubbled with carbogen (95% O2, 5% CO2) to measure isometric tension. Two arteries from each animal were mounted and their responses were averaged. After 20 min equilibration, arteries were stretched to a resting tension of ~2 mN, contracted with KPSS and then maximally contracted with U46619 (U-max, 300 nM, Cayman Chemical, USA). Cumulative concentration-response curves to Ang II, phenylephrine (Sigma Aldrich, USA) and U46619 were obtained with a 20 min washout period between each curve. In another set of experiments, the curves to Ang II, phenylephrine and U46619 were constructed in the absence and presence of L-nitro-arginine methyl ester (L-NAME, 300 μM, Sigma Aldrich, USA), indomethacin (3 μM, Sigma Aldrich, USA), tempol (1 mM, Sigma Aldrich, USA) or tempol (1 mM) + catalase (2500 U/ml, Sigma Aldrich, USA). Each of these drugs was pre-incubated for 30 min before each curve. Responses were expressed as a percentage of the U-max.
4
Tempol Administration for Osteoarthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tempol (Sigma-Aldrich Chemical Company, St. Louis, MO, USA) was prepared and dissolved daily in distilled water in a concentration of 0.4 g/20 mL. The choice of Tempol dose was on a pilot trial (Supplementary Materials ) guided by previously published studies to figure out the appropriate method of administration [40 (link),58 (link),59 (link),60 (link)], and the applicable duration of treatment [31 (link),42 (link),44 (link),56 (link),61 (link)].
In this study, we did not compare with a pharmacological control since the existing approved pharmacological treatments for OA are limited for the relief of OA symptoms (e.g., NSAIDs and corticosteroids), and there is a lack of licensed disease-modifying osteoarthritis drugs (DMOADs) that could slow the narrowing of joint space and provide symptomatic relief.
In this study, we did not compare with a pharmacological control since the existing approved pharmacological treatments for OA are limited for the relief of OA symptoms (e.g., NSAIDs and corticosteroids), and there is a lack of licensed disease-modifying osteoarthritis drugs (DMOADs) that could slow the narrowing of joint space and provide symptomatic relief.
5
Cell Viability Assay under Hypoxia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were treated with DMSO (vehicle control), Tempol (4 mM; Sigma-Aldrich), TMZ (250 μM; Sigma-Aldrich) and the combination of Tempol and TMZ after in vitro hypoxic stress or cell sorting. After incubation at 37 °C for 48 h, the medium was removed from each well, 15 μL 3-94,5-dimethyl-2-yl-2,5-diphenyl-tetrazolium (MTT) (Sigma-Aldrich) solution (2 mg/mL) were added and the plates were incubated at 37 °C for 4 h. The reaction was stopped by the addition of 100 μL of isopropanol/HCl, and the absorbance at 570 nm was recorded on a μQuant plate reader (Bio-Tek).
6
Tempol Effects on Zucker Rat Blood Pressure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The experimental protocols were approved by the Animal Care and Use Committee from The Third Military Medical University and conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication, 8th Edition, 2011). Ten‐week‐old male obese (280 to 300 g) and lean (200 to 220 g) Zucker rats (Charles River Laboratory, Wilmington, MA) were maintained in our animal care facility, with a 12‐hour light/dark cycle and had free access to standard rat chow and drinking water ad libitum. Lean and obese Zucker rats were randomly assigned to control group and tempol group. Control rats were provided plain tap water for drinking. The tempol group rats were provided with tempol (1.0 mmol/L; Sigma, St. Louis, MO) dissolved in the drinking water, which was changed twice a day for 4 weeks.
Systolic, diastolic, and mean arterial blood pressures were measured in conscious animals by using a noninvasive tail‐cuff plethysmography (BP‐98A; Softron, Tokyo, Japan) method. Blood pressure was measured every week until the end of the study.
Systolic, diastolic, and mean arterial blood pressures were measured in conscious animals by using a noninvasive tail‐cuff plethysmography (BP‐98A; Softron, Tokyo, Japan) method. Blood pressure was measured every week until the end of the study.
7
Evaluating Antioxidant Effects in C2C12 Myoblasts and DJ-1 KO Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C2C12 myoblasts were transfected with scramble or Dj1 siRNA and treated with the superoxide dismutase-mimetic and antioxidant tempol (0.1 mM; Sigma). After 48 h, cells were switched to differentiation media and continued to be treated with tempol (0.1 mM) for 96 h.
For in vivo antioxidant treatment, female DJ-1 KO mice and littermate controls were fed a HFD for 3 months and administered NAC for 7 days (10 mg l−1 in drinking water; Sigma). Body weight and ITT were determined before and after treatment.
For in vivo antioxidant treatment, female DJ-1 KO mice and littermate controls were fed a HFD for 3 months and administered NAC for 7 days (10 mg l−1 in drinking water; Sigma). Body weight and ITT were determined before and after treatment.
8
Coronary Vasodilation in Metabolic Syndrome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Coronary small arteries (∼300 µm diameter) were isolated from the epicardial surface of the left ventricular apex and studied in vitro using a Mulvany wire myography (DMT, Aarhus, Denmark) as earlier described [78] (link) (6 Normal and 13 DM + HFD + CKD swine). Vasodilation to the endothelium-dependent vasodilator bradykinin (BK, 10 -10 -10 -6 mol L -1 , Sigma-Aldrich) was measured following precontraction with 10 -6 mol L -1 of the thromboxane-A 2 analog U46619 (Sigma-Aldrich). On adjacent vascular segments, the concentration-response curves for bradykinin were also performed in the presence of the ROS scavengers MPG (10 -5 mol L -1 , Sigma-Aldrich) and TEMPOL (10 -3 mol L -1 , Sigma-Aldrich). Additionally, on small coronary arteries isolated from 5 Normal and 9 DM + HFD + CKD animals concentration-response curves for bradykinin were also attained in the presence of MPG + TEMPOL as well as catalase (10 3 U mL -1 , Sigma-Aldrich) to assess the contribution of H 2 O 2 .
9
Macrophage Activation and Modulation
10
Diabetes Modulation via Epigenetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male C57BLKS/J misty control m/m, db/m, and db/db mice were purchased at 7–8 weeks old, from Japan SLC (Shizuoka, Japan). Animal care and treatment with NIH Guide for Care and Use of Laboratory Animals or the equivalent. To evaluate the effects of DNA demethylation and reactive oxygen species, folic acid (Wako Pure Chemical Industries, ltd, Japan) and Tempol (Merck Millipore ltd, Canada) was administered to 7-week-old db/db mice for 8 weeks. folic acid and Tempol was mixed with drinking water and administered at a dose of 8 mg/kg/day and 2 mmol/l.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!