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Annexin 5 and propidium iodide

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Annexin V and propidium iodide are fluorescent dyes used to detect cell apoptosis and necrosis. Annexin V binds to phosphatidylserine on the cell surface, while propidium iodide stains DNA in cells with compromised cell membranes. These dyes are commonly used together in flow cytometry and microscopy applications to distinguish between early apoptotic, late apoptotic, and necrotic cells.

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Lab products found in correlation

Trypan blue exclusion assay, by Thermo Fisher Scientific (3 mentions) Fcs express software, by De Novo Software (3 mentions) Guava easycyte flowcytometry system, by Merck Group (3 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)

Close spelling variants of this product

FITC Annexin-V/Dead Cell Apoptosis Kit with FITC annexin V and propidium iodide (PI) (2 citations) Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 and propidium iodide (15 citations) Annexin V-FITC and propidium iodide (12 citations) Alexa Fluor 488-labeled annexin V and propidium iodide (2 citations) Dead Cell Apoptosis Kit with Annexin V FITC and propidium iodide (PI) (2 citations) Annexin V-FITC and propidium iodide staining solution (2 citations) FITC-labeled Annexin V and Propidium Iodide Staining Solution (2 citations) Annexin V‐APC and propidium iodide (2 citations) Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit with Alexa Fluor 488 Annexin V and propidium iodide (2 citations) Annexin V-FITC and Propidium Iodide (PI) Kit (2 citations)

3 protocols using annexin 5 and propidium iodide

1

Cell Viability and Cell Death Analyses

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Cell viability was determined by trypan blue exclusion assay (Invitrogen) or by flow cytometry of cells stained with the fluorescent DNA intercalator TO-PRO-3 (Invitrogen). The colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed as previously described (59 (link)). For cell cycle and death analyses, flow cytometry of cells stained with annexin V and propidium iodide (Invitrogen) was performed using the Guava EasyCyte flowcytometry system (MilliporeSigma), as previously described (66 (link)). Data were analyzed by FCS EXPRESS software (De Novo Software).
Wu P.K., Hong S.K., Chen W., Becker A.E., Gundry R.L., Lin C.W., Shao H., Gestwicki J.E, & Park J.I. (2020). Mortalin (HSPA9) facilitates BRAF–mutant tumor cell survival by suppressing ANT3-mediated mitochondrial membrane permeability. Science signaling, 13(622), eaay1478.
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2

Evaluating Cell Viability and Apoptosis

Cited 1 time
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Cell viability was determined by trypan blue exclusion assay (Invitrogen) or by flow cytometry of cells stained with the fluorescent DNA intercalator TO-PRO 3 (Invitrogen). The colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) assay was performed as previously described19 (link). For cell cycle and death analyses, flow cytometry of cells stained with annexin V and propidium iodide (Invitrogen) was performed using the Guava EasyCyte flowcytometry system (MilliporeSigma, Billerica, MA, USA), as previously described47 (link). Data were analyzed by FCS EXPRESS software (De Novo Software, Los Angeles, CA, USA).
Wu P.K., Hong S.K., Starenki D., Oshima K., Shao H., Gestwicki J.E., Tsai S, & Park J.I. (2020). Mortalin/HSPA9 targeting selectively induces KRAS tumor cell death by perturbing mitochondrial membrane permeability. Oncogene, 39(21), 4257-4270.
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3

Evaluating Cell Viability and Apoptosis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell viability was determined by trypan blue exclusion assay (Invitrogen) or by flow cytometry of cells stained with the fluorescent DNA intercalator TO-PRO 3 (Invitrogen). The colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) assay was performed as previously described19 (link). For cell cycle and death analyses, flow cytometry of cells stained with annexin V and propidium iodide (Invitrogen) was performed using the Guava EasyCyte flowcytometry system (MilliporeSigma, Billerica, MA, USA), as previously described47 (link). Data were analyzed by FCS EXPRESS software (De Novo Software, Los Angeles, CA, USA).
Wu P.K., Hong S.K., Starenki D., Oshima K., Shao H., Gestwicki J.E., Tsai S, & Park J.I. (2020). Mortalin/HSPA9 targeting selectively induces KRAS tumor cell death by perturbing mitochondrial membrane permeability. Oncogene, 39(21), 4257-4270.
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