Human il 6 elisa kit

Manufactured by MultiSciences Biotech

Sourced in China

The Human IL-6 ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human interleukin-6 (IL-6) levels in biological samples. It utilizes a specific antibody coated on a microplate to capture the target analyte from the sample. A detection antibody is then used to quantify the amount of bound IL-6 through a colorimetric reaction.

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ELISAs (2 citations)

8 protocols using human il 6 elisa kit

Ferulic Acid Modulates Inflammatory Response

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RPMI-1640 culture medium and foetal bovine serum were obtained from Gibco. Ferulic acid (FA), lipopolysaccharide (LPS), Phorbol-12-myristate-13-acetate (PMA), thiazolyl blue tetrazolium bromide (MTT), and 3-methyladenine (3-MA) were purchased from Sigma-Aldrich. MCC950 was purchased from Abmole Bioscience Inc. DCFH-DA was purchased from Beyotime Biotechnology Co., Ltd. The reverse transcription kits and SYBR Premix Ex Taq were purchased from Takara Biomedical Technology. The antibodies against Microtubule Associated Protein 1 Light Chain 3 Beta (LC3), Sequestosome 1 (p62), Beclin-1, NLRP3, pro-caspase-1, caspase-1, pro-Interleukin 1 Beta (IL-1β), IL-1β and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were purchased from ABclonal Technology. The Human IL-6 ELISA Kit, Human IL-1β ELISA kit and human TNF-α ELISA Kit were purchased from Multisciences (Lianke) Biotech, Co., Ltd. All other reagents used were obtained from local commercial sources.

Liu Y., Shi L., Qiu W, & Shi Y. (2022). Ferulic acid exhibits anti-inflammatory effects by inducing autophagy and blocking NLRP3 inflammasome activation. Molecular & Cellular Toxicology, 18(4), 509-519.

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Urinary Biomarker Analysis in Kidney Injury

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Following centrifugation of urine for 20 min, the supernatant was collected for ELISA assay. Mouse Urinary Albumin (UALB) ELISA Kit was used to measure the UALB. Urine neutrophil gelatinase-associated lipocalin (NGAL) was detected by Mouse NGAL ELISA Kit. We performed the detection of urinary kidney injury molecule 1 (KIM-1) using mouse KIM-1 ELISA Kit. The three ELISA kits were purchased from Wuhan Fine Biotech (Wuhan, China).
The levels of interleukin-6 (IL-6), IL-1β, tumor necrosis factor alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) in renal cortex and HK2 cells were detected according to ELISA kit instructions. The kits used in this part were purchased from MULTI SCIENCES (Zhejiang, China) and they included Mouse IL-6 ELISA kit, Human IL-6 ELISA kit, mouse IL-1β ELISA kit, human IL-1β ELISA kit, mouse TNF-α ELISA kit, human TNF-α ELISA kit, mouse MCP-1 ELISA kit and Human MCP-1 ELISA Kit.

Li X.J., Liu T, & Wang Y. (2023). Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway. Journal of Natural Medicines, 78(1), 53-67.

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Quantifying Interleukin-6 Levels

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The Human IL-6 ELISA Kit (MULTI SCIENCES) was used to measure the expression of interleukin 6 (IL-6) in the medium. The culture medium was collected 3 days after cell culture (13 000 r/min, 5 min). ELISA was performed according to the manufacturer’s instructions. A microplate reader was used to measure the absorbance at 450 nm.

Deng L., Zhang X., Xiang X., Xiong R., Xiao D., Chen Z., Liu K, & Feng G. (2021). NANOG Promotes Cell Proliferation, Invasion, and Stemness via IL-6/STAT3 Signaling in Esophageal Squamous Carcinoma. Technology in Cancer Research & Treatment, 20, 15330338211038492.

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Cytokine Levels Under SFP and DSFP-45

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The effects of SFP and DSFP-45 on the levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, were studied by ELISA. The level of IL-1β was measured using the Human IL-1β ELISA Kit (EHC002b.48, NeoBioscience Technology Co., Ltd., Shenzhen, China) according to the manufacturer’s protocol. The content of IL-6 was determined using the Human IL-6 ELISA Kit (70-EK106/2-96, Multi Sciences Biotech Co., Ltd., Hangzhou, China). The amount of TNF-α was detected by the Human TNF-α ELISA Kit (70-EK182-96, Multi Sciences Biotech Co., Ltd., Hangzhou, China).

Yao W., Yong J., Lv B., Guo S., You L., Cheung P.C, & Kulikouskaya V.I. (2023). Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment. Marine Drugs, 21(8), 430.

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Cytokine and Receptor ELISA Quantification

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sB7-H5 and cytokines were detected using ELISA following the Manufacturer's manual. The Human Platelet receptor Gi24 (C10orf54) ELISA kit (CSB-EL002961HU, Cusabio technology, Wuhan, Hubei, China), Human IL-6 ELISA Kit (70-EK106/2-96, MultiSciences Biotech, Hangzhou, Zhejiang, China), Human IL-10 ELISA Kit (70-EK110/2-96, MultiSciences Biotech, Hangzhou, Zhejiang, China), and Human TNF-α ELISA Kit (70-EK182-96, MultiSciences Biotech, Hangzhou, Zhejiang, China) were used.

Xu R., Gong J., Chen W., Jin Y, & Huang J. (2021). Soluble B7-H5 Is a Novel Diagnostic, Severity, and Prognosis Marker in Acute Pancreatitis. BioMed Research International, 2021, 1223850.

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PGE2 and IL-6 ELISA Protocols

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Commercial kits, including Prostaglandin E2 (PGE2) Competitive ELISA Kit and Human IL-6 ELISA Kit, were bought from MultiSciences (Hangzhou, China). The procedures of ELISA were performed in accordance with respective specifications.

Wei W., Mu H., Cui Q., Yu P., Liu T., Wang T, & Sheng L. (2023). CircTBX5 knockdown modulates the miR-558/MyD88 axis to alleviate IL-1β-induced inflammation, apoptosis and extracellular matrix degradation in chondrocytes via inactivating the NF-κB signaling. Journal of Orthopaedic Surgery and Research, 18, 477.

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Quantifying Inflammatory Cytokines in Arthritis

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The serum levels of TNF-α, IL-17, and IL-23 from CIA rats were measured by ELISA kits in accordance with the manufacturer's instructions. The same experimental method was also applied to detect the concentrations of IL-1β and IL-6 in the supernatant of HFLS-RA cells. The cells were treated with concentrations of 40, 80, and 160 μg/mL and stimulated by TNF-α for 24 h. Then, the cells were centrifuged to obtain a cell supernatant for ELISA. Standard curves were calculated according to the OD value so that we could determine the concentrations of inflammatory cytokines. We purchased the following ELISA kits from Multi Sciences (Hangzhou, China): rat TNF-α ELISA Kit (70-EK382/3-96), rat IL-17A ELISA Kit (70-EK317/3-96), human IL-6 ELISA Kit (70-EK106/2-96), and human IL-1β ELISA Kit (70-EK101B-96). An IL-23 ELISA kit (E-EL-R0569c) was obtained from Elabscience (Wuhan, China).

Tang L.D., Wang J.Y., Zhang Y., Chen X.Y., Zhang L, & Yuan Y. (2023). Iridoid from Eucommia ulmoides Oliv. Exerts Antiarthritis Effects by Inhibiting the JAK2/STAT3 Signaling Pathway In Vivo and In Vitro. Evidence-based Complementary and Alternative Medicine : eCAM, 2023, 4167906.

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Quantification of IL-6 Levels

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The concentrations of IL‐6 were detected using a commercial human IL‐6 ELISA kit (Multisciences (Lianke) Biotech Co. Ltd, Hangzhou, China), in accordance with the manufacturer's instructions.

Xiao F., Liu Y., Zhang Z., Wang L., Wang T, & Wang X. (2020). Tobacco extracts promote PD‐L1 expression and enhance malignant biological differences via mTOR in gefitinib‐resistant cell lines. Thoracic Cancer, 11(8), 2237-2251.

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