Pl50 instrument

2 mg mL⁻¹ solution of each polymer in DMF containing 0.1% (w:v) LiBr were incubated for 16 h at room temperature. Analyte samples were filtered through a nylon membrane with 0.22 μm pore size before injection. An Agilent PL50 instrument equipped with differential refractive index (DRI) and UV detectors was used for all the measurements. The system was equipped with two x PolarGel M columns (300 × 7.5 mm) and a PolarGel 5 µm guard column, connected in series. The eluent was DMF containing 0.1% LiBr. All experiments were performed at a flow rate of 1 ml min⁻¹ at 50 °C. Experimental molar mass (M_n,SEC) and dispersity (Đ) values of synthesized polymers were determined by comparison with poly(methyl methacrylate) standards (Agileny EasyVials) using Agilent GPC/SEC software. All data were elaborated using Origin.

GPC was measured using an Agilent PL50 instrument with a differential refractive index detector. The instrument contained two PolarGel H columns (300 mm × 7.5 mm) and a PolarGel 5 μm guard column. DMF with 0.1% LiBr additive was used as the eluent. The system ran at 1 mL min⁻¹ (50 °C), with an injection volume of 100 μL. The samples were prepared by filtering them through 0.22 μm pore size PTFE membranes, before injection. Agilent EasyVial poly(methyl methacrylate) standards were used to calibrate the instrument and output data were analysed using Agilent GPC/SEC software.

GPC was measured using an Agilent PL50 instrument with a differential refractive index detector. The instrument contained two PolarGel H columns (300 mm × 7.5 mm) and a PolarGel 5 μm guard column. DMF with 0.1% LiBr additive was used as the eluent. The system ran at 1 mL min⁻¹ (50 °C), with an injection volume of 100 μL. The samples were prepared by filtering them through 0.22 μm pore size PTFE membranes, before injection. Agilent EasyVial poly(methyl methacrylate) standards were used to calibrate the instrument and output data were analyzed using Agilent GPC/SEC software.