The rPLM, gPLM, and bPLM photoluminescent materials were procured from Dalian Luminescent Technology Co., Ltd., Dalian, China. The rice variety used was YangJing 687. Acetone, indantrione, glacial acetic acid, tris(phenyl)tetrazolium chloride, and o-phenylenediamine were purchased from Beijing Fine Chemicals Co., Ltd., Beijing China. Trichloroacetic acid, thiobarbituric acid, anthrone, sulfosalicylic acid, and Coomassie Brilliant Blue G-250 was obtained from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Guaiacol was sourced from Shanghai Source Leaf Biotechnology Co., Ltd., Shanghai, China. Anhydrous ethanol and barium chloride were supplied by Xilong Scientific Co., Ltd., Shantou, China. All reagents were of analytical grade.
Thiobarbituric acid
Manufactured by Sinopharm
Sourced in China
Thiobarbituric acid is a chemical compound used as a reagent in analytical laboratory procedures. It is primarily utilized in colorimetric assays to detect and quantify the presence of certain compounds, such as lipid peroxidation products. Thiobarbituric acid reacts with these target compounds to produce a colored complex, which can then be measured using spectrophotometric techniques.
Automatically generated - may contain errors
Lab products found in correlation
Trichloroacetic acid, by Sinopharm (4 mentions)
Methanol, by Sinopharm (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Sinopharm (2 mentions)
Ethanol, by Sinopharm (2 mentions)
Chloroform, by Sinopharm (2 mentions)
Anhydrous ethanol, by Xilong (1 mentions)
Sulfosalicylic acid, by Sinopharm (1 mentions)
Coomassie brilliant blue g 250, by Sinopharm (1 mentions)
Anthrone, by Sinopharm (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Aluminum chloride, by Sinopharm (1 mentions)
Iron sulfate, by Sinopharm (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Polyvinylpyrrolidone, by Sinopharm (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
L borneol, by Merck Group (1 mentions)
Anhydrous na2so4, by Sinopharm (1 mentions)
Anhydrous sodium carbonate, by Sinopharm (1 mentions)
8 protocols using thiobarbituric acid
1
Rice Photoluminescent Material Characterization
2
Phytochemical and Antioxidant Analysis
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SA, sodium hydroxide (NaOH), thiobarbituric acid (TBA), hydrochloric acid (HCl), trichloroacetic acid (TCA), methanol, ethanol, aluminum chloride (AlCl3), polyvinylpyrrolidone (PVP), and iron sulfate were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Folin–Ciocalteu reagent, gallic acid (GA), rutin, L-ascorbic acid (L-AsA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and dithiothreitol (DTT) were obtained from Sigma-Aldrich, Chemical Co. (Louis, MO, USA).
3
Comprehensive Chemical Characterization
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n-Hexane (99%), l-borneol (97%), xanthoxylin (97%) and 1,1-diphenyl-2-picrylhydrazyl (DPPH, 95%) were purchased from Sigma-Aldrich (Shanghai, China). β-carotene was purchased from Fluka Chemie (Buchs, Switzerland). Thiobarbituric acid, anhydrous Na2SO4, and all of the applied solvents (analysis purity) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). For Linear retention indices (RIs) determination, a hydrocarbon mixture (C8–C30n-alkanes) purchased from Supelco (Ballefonte, USA) was used to perform in the same condition as real sample determination.
For the measurement of response factors, the chemicals used were: l-borneol, ledol, γ-eudesmol, β-eudesmol, 1-octen-3-ol, linalool, nerolidol, 3-octanol and α-terpineol for alcohols; pentadecanal for aldehydes; camphor, zierone and xanthoxylin for ketones; hexadecanoic acid for acids; β-pinene for monoterpene hydrocarbons; α-muurolene, δ-cadinene caryophyllene and α-caryophyllene for sesquiterpene hydrocarbons; caryophyllene epoxide for oxides; 1,2-dihydro-1,1,6-trimethyl-naphthalene for aromatic hydrocarbons.
For the measurement of response factors, the chemicals used were: l-borneol, ledol, γ-eudesmol, β-eudesmol, 1-octen-3-ol, linalool, nerolidol, 3-octanol and α-terpineol for alcohols; pentadecanal for aldehydes; camphor, zierone and xanthoxylin for ketones; hexadecanoic acid for acids; β-pinene for monoterpene hydrocarbons; α-muurolene, δ-cadinene caryophyllene and α-caryophyllene for sesquiterpene hydrocarbons; caryophyllene epoxide for oxides; 1,2-dihydro-1,1,6-trimethyl-naphthalene for aromatic hydrocarbons.
4
Determination of H2O2 and MDA Levels
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H2O2 content was determined based on the method of Brennan and Frenkel, [59 (link)] with slight modifications. Four mL of 10 mM 3-amino-1,2,4-triazole (Bio Basic Inc, Toronto, Canada) and 0.2 g of fresh leaves were ground into a homogenate and centrifuged at 6000× g for 25 min. Two mL of the supernatant was added to 1 mL of 0.1% titanium tetrachloride (Shanghai Lingfeng Chemical Reagent Co., Ltd., Shanghai, China) solution containing 20% H2SO4, mixed, centrifuged, and the absorbance of the supernatant was measured with a spectrophotometer at 410 nm.
Two mL of 5% trichloroacetic acid was added to 0.1 g of fresh leaves to form a homogenate. The concentration of thiobarbituric acid (Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China) reactive substances was determined to estimate MDA content [60 (link)].
Two mL of 5% trichloroacetic acid was added to 0.1 g of fresh leaves to form a homogenate. The concentration of thiobarbituric acid (Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China) reactive substances was determined to estimate MDA content [60 (link)].
5
Antioxidant Capacity of Phytochemicals
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Naringenin, naringin, hesperetin, and dihydromyricetin (≥98%) were purchased from Xi’an Natural Field Biological Technology Co. Ltd. (Xi’an, China). 2,2′-azino-bis(3-ethylbenzthiazo-line-6-sulphonic acid) and 2,2-diphenyl-1-picrylhydrazyl were obtained from Sigma-Aldrich Company (St. Louis, MO, USA). The ingredients for cookie-making were purchased from a local supermarket in Shanghai, China. 1,1,3,3-Tetraethoxypropane and polyphenol standards were bought from ANPEL Scientific Instrument Co. (Shanghai, China). Other chemicals including anhydrous sodium carbonate, ethylene diamine tetraacetic acid, Folin-Ciocalteu’s phenol reagent, potassium peroxodisulphate, thiobarbituric acid, and trichloroacetic acid were of analytical grade and obtained from Sinopharm Chemical Reagent Co. (Shanghai, China).
6
Paeonia ostii Seed Extraction Protocol
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The peony seeds (Paeonia ostia, 10 kg) were obtained from Shandong Seed Industry Group CO., Ltd. (Jinan, China). Quillaja saponin was purchased from Shanxi Haosen Biological Technology Co., Ltd. (Xi’an, China). Protein Markers (14.4~97.4 kDa) were bought from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China) Mercaptoethanol, Bromophenol blue, Coomasil bright blue, glacial acetic acid, isoctane, isopropyl alcohol, methanol, n-butanol, thiobarbituric acid, sodium azide, trichloroacetic acid, and trichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents were of analytical grade.
7
Grass Carp Processing and Analysis
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Live grass carp (2.5–3.0 kg) was purchased from the Huazhong Agricultural University market (Wuhan, China). Arowana multipurpose wheat core wheat flour (Yihai Kerry Arowana Cereals and Oils Food Co., Ltd., Shanghai, China), MT200 modified starch (Tianjin Dingfeng Starch Development Co., Ltd., Tianjin, China), konjac glucomannan (food grade) (Hefei Shengrun Biological Products Co., Ltd., Hefei, China) and rapeseed oil (Shandong Luhua Group Co., Ltd., Yantai, China) were used. Salt and baking powder were purchased from Zhongbai Supermarket of Huazhong Agricultural University (Wuhan, China).
Petroleum ether (30–60 °C), HCl, chloroform, Sudan Red B, acetone, NaBH4, methanol, thiobarbituric acid, disodium EDTA, 1,1,3,3-tetraethoxypropane, formic acid, dichloromethane and hexane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Carboxymethyl lysine (CML), carboxyethyl lysine (CEL) and methylglyoxalimidazolone (MG-H1) standards were purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Acrylamide standard was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
Petroleum ether (30–60 °C), HCl, chloroform, Sudan Red B, acetone, NaBH4, methanol, thiobarbituric acid, disodium EDTA, 1,1,3,3-tetraethoxypropane, formic acid, dichloromethane and hexane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Carboxymethyl lysine (CML), carboxyethyl lysine (CEL) and methylglyoxalimidazolone (MG-H1) standards were purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Acrylamide standard was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
8
Evaluating Antioxidant Capacity of Rosemary Extract
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Ethanol, NaOH, NaCl, potassium ferricyanide, FeCl3, 2-thiobarbituric
acid, trichloroacetic acid (TCA), chloroform (99.5%), mEthanol,
ethylenediamine tetraacetic acid disodium salt, isooctane, acetic acid,
Na2HPO4·12H2O,
NaH2PO4, and 2-propanol were obtained from Sinopharm
Chemical Reagent (Shanghai, China). P-anisidine purity 99% was obtained
from Aladdin Industrial Corporation (Shanghai, China).
1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biological
Technology (Shanghai, China). n-Hexane for chromatography (grade 99.5%)
was purchased from Tianjin Guangfu Fine Chemical Research Institute (China).
Borontrifluo-ride-mEthanol (BF3-MeOH purity>50%
(14wt/v%) was obtained from ANPEL Scientific Instruments (Shanghai,
China).
Approximately 350 fresh duck (Anas platyrhucus) eggs weighing
65–75 g, which were retrieved within 3 days of laying. RE was bought from
Henan Yuzhong Biotechnology, China. RE was extracted from dried rosemary
(Rosmarinus oficinalis L.) which is
water-soluble/oil-soluble materials (supplementary Fig. S1). In addition, the
extract contains the main components such as rosmanol, carnosol, and carnosic
acid. Moreover, the studied RE composition is in agreement with Nakatani and Inatani (1981) (link), Zhang et al. (2010) (link), and Rocío Teruel et al. (2015) (link) who
proved that carnosol, rosmanol, and carnosic acid are the main compound in the
RE.
acid, trichloroacetic acid (TCA), chloroform (99.5%), mEthanol,
ethylenediamine tetraacetic acid disodium salt, isooctane, acetic acid,
Na2HPO4·12H2O,
NaH2PO4, and 2-propanol were obtained from Sinopharm
Chemical Reagent (Shanghai, China). P-anisidine purity 99% was obtained
from Aladdin Industrial Corporation (Shanghai, China).
1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biological
Technology (Shanghai, China). n-Hexane for chromatography (grade 99.5%)
was purchased from Tianjin Guangfu Fine Chemical Research Institute (China).
Borontrifluo-ride-mEthanol (BF3-MeOH purity>50%
(14wt/v%) was obtained from ANPEL Scientific Instruments (Shanghai,
China).
Approximately 350 fresh duck (Anas platyrhucus) eggs weighing
65–75 g, which were retrieved within 3 days of laying. RE was bought from
Henan Yuzhong Biotechnology, China. RE was extracted from dried rosemary
(Rosmarinus oficinalis L.) which is
water-soluble/oil-soluble materials (supplementary Fig. S1). In addition, the
extract contains the main components such as rosmanol, carnosol, and carnosic
acid. Moreover, the studied RE composition is in agreement with Nakatani and Inatani (1981) (link), Zhang et al. (2010) (link), and Rocío Teruel et al. (2015) (link) who
proved that carnosol, rosmanol, and carnosic acid are the main compound in the
RE.
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