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Cetuximab

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Cetuximab is a monoclonal antibody that binds to the epidermal growth factor receptor (EGFR). It is a laboratory product used for research purposes.

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Lab products found in correlation

Live dead viability dye, by BD (2 mentions) Cetuximab custom conjugated to allophycocyanin, by BD (2 mentions) Custom biotinylated trastuzumab and streptavidin, by BD (2 mentions) Anti phospho erk1 2 t202 y204, by Cell Signaling Technology (1 mentions) Anti phospho p38 t180 y182, by Cell Signaling Technology (1 mentions) Anti phospho sapk jnk t183 y185, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti sapk jnk, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti p38, by Cell Signaling Technology (1 mentions)

4 protocols using cetuximab

1

Antibody Characterization for Cellular Signaling

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Antibodies used in this work include: Cetuximab (Creative Diagnostics Cat# AIT-19034, RRID:AB_2489605; Erbitux), anti-Erk1/2 (Cell Signaling Technology Cat# 4695, RRID:AB_390779), anti-phospho-ERK1/2 (T202/Y204) (Cell Signaling Technology Cat# 4370, RRID:AB_2315112), anti-p38 (Cell Signaling Technology Cat# 8690, RRID:AB_10999090), anti-phospho-p38 (T180/Y182) (Cell Signaling Technology Cat# 4511, RRID:AB_2139682), anti-SAPK/JNK (Cell Signaling Technology Cat# 9252, RRID:AB_2250373), anti-phospho-SAPK/JNK (T183/Y185) (Cell Signaling Technology Cat# 4668 P, RRID:AB_10831195), anti-MYC (Cell Signaling Technology Cat# 13987, RRID:AB_2631168), anti-MIZ1 (14300, Cell Signalling), anti-HBD1 (Abcam Cat# ab14425, RRID:AB_301206), anti-actin (Sigma-Aldrich Cat# A2066, RRID:AB_476693), anti-mouse IgG-POX (GE Healthcare Cat# NXA931-1ml, RRID:AB_772209), and anti-rabbit IgG-POX (GAR/IgG(H + L)/PO, Nordic Immunology).
Bonamy C., Sechet E., Amiot A., Alam A., Mourez M., Fraisse L., Sansonetti P.J, & Sperandio B. (2018). Expression of the human antimicrobial peptide β-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells. Scientific Reports, 8, 18043.
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2

Multiparameter Flow Cytometry for CAR T-cell Analysis

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Peripheral blood, bone marrow aspirate and whole cerebrospinal fluid were stained as previously described [7 ] or as follows. Peripheral blood and bone marrow aspirates were treated to lyse red blood cell (Invitrogen Cat# 00-4333-57) prior to persistence tracking via flow staining. Isolated white blood cells and whole cerebrospinal fluid were stained with a viability dye (BD Cat# 564407) and monoclonal antibodies specific for the following human markers: CD3 (BD Cat# 652356), CD4 (BD Cat# 562658), CD8 (BD Cat# 560662), CD19 (BD Cat# 340720), and CD36 (BD Cat# 555454). CAR expression on the T cells was quantified using Cetuximab (Creative Diagnostics Cat# TAB-003) custom-conjugated to allophycocyanin by BD Biosciences to detect the transduction marker, truncated EGFR.
Ceppi F., Wilson A.L., Annesley C., Kimmerly G.R., Summers C., Brand A., Seidel K., Wu Q.V., Beebe A., Brown C., Mgebroff S., Lindgren C., Rawlings-Rhea S.D., Huang W., Pulsipher M.A., Wayne A.S., Park J.R., Jensen M.C, & Gardner R.A. (2022). Modified manufacturing process modulates CD19CAR T-cell engraftment fitness and leukemia-free survival in pediatric and young adult subjects. Cancer immunology research, 10(7), 856-870.
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3

Immunophenotyping of CAR T Cells in CSF

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Immunophenotyping of surface markers on cells isolated from the CSF correlative specimens was performed using standard staining and flow cytometry techniques, using live/dead viability dye (BD Biosciences) and the following fluorophore-conjugated anti-human monoclonal antibodies: CD3, CD4, CD8a, and CD36 (BD Biosciences). CAR T cell expression was quantified using cetuximab custom-conjugated to allophycocyanin (BD Biosciences) for detection of the EGFRt tag. Cells were also stained with custom-biotinylated trastuzumab and streptavidin (BD Bioscience) for detection of a HER2 tag not relevant to this trial. CAR T cells were defined as singlets/lymphocytes/viable CD36-/CD3+/EGFRt+HER2-. CD4 and CD8 expression in the CAR+ and CAR- populations was evaluated. Representative flow gating is in Supplementary Figure 12. Antibodies for CAR detection in CSF: CD3 (BD Biosciences Cat# 562426, RRID:AB_11152082 or BD Biosciences Cat# 652356, RRID:AB_2868395), CD4 (BD Biosciences Cat# 562658, RRID:AB_2744420), CD8a (BD Biosciences Cat# 560662, RRID:AB_1727513), CD36 (BD Biosciences Cat# 555454, RRID: AB_2291112), cetuximab (Creative Diagnostics Cat# TAB-003, RRID:AB_2459632) custom-conjugated to allophycocyanin by BD Biosciences.
Vitanza N.A., Wilson A.L., Huang W., Seidel K., Brown C., Gustafson J.A., Yokoyama J.K., Johnson A.J., Baxter B.A., Koning R.W., Reid A.N., Meechan M., Biery M.C., Myers C., Rawlings-Rhea S.D., Albert C.M., Browd S.R., Hauptman J.S., Lee A., Ojemann J.G., Berens M.E., Dun M.D., Foster J.B., Crotty E.E., Leary S.E., Cole B.L., Perez F.A., Wright J.N., Orentas R.J., Chour T., Newell E.W., Whiteaker J.R., Zhao L., Paulovich A.G., Pinto N., Gust J., Gardner R.A., Jensen M.C, & Park J.R. (2022). Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety. Cancer Discovery, 13(1), 114-131.
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4

Immunophenotyping of CAR T-cells in CSF

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Immunophenotyping of surface markers on cells isolated from the CSF correlative specimens was performed using standard staining and flow cytometry techniques, using live/dead viability dye (BD Biosciences) and the following fluorophore-conjugated antihuman monoclonal antibodies: CD3, CD4, CD8a, and CD36 (BD Biosciences). CAR T-cell expression was quantified using cetuximab custom conjugated to allophycocyanin (BD Biosciences) for detection of the EGFRt tag. Cells were also stained with custom-biotinylated trastuzumab and streptavidin (BD Biosciences) for the detection of a HER2 tag not relevant to this trial. CAR T cells were defined as singlets/lymphocytes/viable CD36−/CD3+/EGFRt+HER2−. CD4 and CD8 expression in the CAR+ and CAR− populations was evaluated. Representative flow gating is in Supplementary Fig. S12. The following antibodies were used for CAR detection in CSF: CD3 (BD Biosciences, cat. #562426, RRID:AB_11152082 or BD Biosciences, cat. #652356, RRID:AB_2868395), CD4 (BD Biosciences, cat. #562658, RRID:AB_2744420), CD8a (BD Biosciences, cat. #560662, RRID:AB_1727513), CD36 (BD Biosciences, cat. #555454, RRID: AB_2291112), cetuximab (Creative Diagnostics, cat. #TAB-003, RRID:AB_2459632) custom conjugated to allophycocyanin by BD Biosciences.
Vitanza N.A., Wilson A.L., Huang W., Seidel K., Brown C., Gustafson J.A., Yokoyama J.K., Johnson A.J., Baxter B.A., Koning R.W., Reid A.N., Meechan M., Biery M.C., Myers C., Rawlings-Rhea S.D., Albert C.M., Browd S.R., Hauptman J.S., Lee A., Ojemann J.G., Berens M.E., Dun M.D., Foster J.B., Crotty E.E., Leary S.E., Cole B.L., Perez F.A., Wright J.N., Orentas R.J., Chour T., Newell E.W., Whiteaker J.R., Zhao L., Paulovich A.G., Pinto N., Gust J., Gardner R.A., Jensen M.C, & Park J.R. (2022). Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety. Cancer Discovery, 13(1), 114-131.
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