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Uv 2450 spectrophotometer

Manufactured by Jasco

The UV-2450 spectrophotometer is a device designed to measure the absorbance or transmittance of light by a sample across a range of ultraviolet and visible wavelengths. It is a tool used for analytical and quantitative analysis applications in various scientific fields.

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3 protocols using uv 2450 spectrophotometer

1

Spectroscopy Techniques for Biomolecular Analysis

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UV, fluorescence, and CD spectra were recorded using a Shimadzu UV-2450 spectrophotometer, a JASCO FP-6600 spectrofluorometer, and a JASCO J-1500 circular dichroism spectrometer, respectively.
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2

Characterization of Molecular Assemblies

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NMR spectra were measured with a Bruker DPX-400 spectrometer. ESI mass spectra were obtained on Thermo Scientific™ Exactive™ Bench-Top LC-MS Spectrometer and Thermo Scientific™ Exactive™ Plus Bench-Top LC-MS Spectrometer. FT-IR spectroscopy was carried out with a Nicolet 6700 FT-IR spectrometer. Molecular assembly formation in solutions was analyzed by dynamic light scattering (DLS) by using Photal DLS-8000. UV and CD spectra were obtained on a Shimadzu UV-2450 spectrophotometer and a JASCO J600 circular dichroism spectrometer, respectively. The UV and CD spectra were obtained using a quartz optical cell with 1 cm path length at a concentration of 0.05 mM.
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3

Analytical Techniques for Glycolipids and BChl c

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Analysis of glycolipids by a HPLC system equipped with an evaporative light scattering detector (ELSD) was performed with a Shimadzu LC-20AT pump and an ELSD-LT II detector by the control of column temperature with a Shimadzu CTO-20AC column oven. HPLC analysis of BChl c was carried out with a Shimadzu LC-20AT pump and an SPD-M20A detector. Liquid chromatography-mass spectrometry (LC-MS) was done with a Shimadzu LCMS-2020 system equipped with an electrospray ionization probe (ESI). Visible absorption and circular dichroism (CD) spectra were measured with a Shimadzu UV-2450 spectrophotometer and a JASCO J-820 spectropolarimeter, respectively.
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