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Unlq 10 column total rna isolation kit

Manufactured by Sangon
Sourced in China

The UNlQ-10 Column Total RNA Isolation Kit is a laboratory equipment designed for the extraction and purification of total RNA from various biological samples. It utilizes a silica-based column method to efficiently capture and isolate RNA molecules, allowing for the recovery of high-quality RNA for downstream applications.

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3 protocols using unlq 10 column total rna isolation kit

1

Isolation and Analysis of RNA

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Con A was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China), batch no. C8110. Chloral hydrate and UNlQ-10 Column Total RNA Isolation Kit were gained from Sangon Biotech Co., Ltd. (Shanghai, China), batch number: A600288 and B511321. Maxima Reverse Transcriptase was gotten from Thermo Fisher Scientific (China) Co., Ltd. (Shanghai, China), batch no. EP0743.
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2

Isolation and RT-PCR for RNA Analysis

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Con A (batch number: C8110) was gained from Solarbio Science & Technology Co., Ltd. (Beijing, China). Chloral hydrate (batch number: A600288) and UNlQ‐10 Column Total RNA Isolation Kit (batch number: B511321) were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). Maxima Reverse Transcriptase (batch number: EP0743) was bought from Thermo Fisher Scientific (China) Co., Ltd. (Shanghai, China).
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3

Oxidative Stress Evaluation in Animal Models

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Con A was bought from Solarbio Science & Technology Co., Ltd. (Beijing, China, catalog number: C8110). Malondialdehyde (MDA) assay kit (TBA method), Alanine aminotransferase (ALT) Assay Kit, and Aspartate aminotransferase (AST) Assay Kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China, catalog number: A003-1-2, C009-2-1 and C010-2-1). Total Nitric Oxide (NO) Assay Kit was gotten from Beyotime Biotechnology (Shanghai, China, catalog number: S0021S). Chloral hydrate, UNlQ-10 Column Total RNA Isolation Kit and 2X SYBR Abstart Master Mix was purchased from Sangon Biotech Co., Ltd. (Shanghai, China, catalog number: A600288, B511321 and B110032). The Total Protein Extraction Kit (BOSTER Biological & Technology Co. Ltd., China, catalog number: AR0146), SDS–polyacrylamide gel electrophoresis (SDS-PAGE, Beyotime Biological Technology Co. Ltd., China, catalog number: P0012A), PVDF membranes (Millipore, USA, catalog number: R1CB73920), High-sig ECL Western Blotting Substrate (Tanon Science & Technology Co., Ltd., China, catalog number:180-501), Anti-p38 Rabbit pab, Mapk3k13 Rabbit pab, HRP-conjugated Goat Anti-Rabbit IgG (Additional file 1: Table S1) were used to detect the expression of target protein.
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