Anti rab7 d95f2

Manufactured by Cell Signaling Technology

Sourced in Canada

Anti-Rab7 D95F2 is a rabbit monoclonal antibody that targets the Rab7 protein. Rab7 is a small GTPase that regulates late endosome and lysosome biogenesis and function.

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Lab products found in correlation

Anti lamp1, by Merck Group (2 mentions) Mabt837, by Merck Group (2 mentions) Lsm 710 axioobserver confocal microscope, by Zeiss (2 mentions) Fluoromount g, by Southern Biotech (2 mentions) Folch fraction 1, by Merck Group (1 mentions) Anti histone h3, by Abcam (1 mentions) Anti lamp1, by Abcam (1 mentions) Anti β tubulin aa2, by Merck Group (1 mentions) Anti kif5b, by Abcam (1 mentions) Cell fractionation kit, by Cell Signaling Technology (1 mentions) Non targeting control sirna, by Horizon Discovery (1 mentions) Anti actin ac 74, by Merck Group (1 mentions) Anti ha ha 7, by Merck Group (1 mentions) Brain ps, by Avanti Polar Lipids (1 mentions) Egg pa, by Avanti Polar Lipids (1 mentions) Bovine liver phosphatidylinositol pi, by Avanti Polar Lipids (1 mentions) Phosphorylated p38 and total p38, by Cell Signaling Technology (1 mentions) Myc sc 40, by Santa Cruz Biotechnology (1 mentions) Lamp1 sc 19992, by Santa Cruz Biotechnology (1 mentions) Vamp8, by Synaptic Systems (1 mentions)

Spelling variants of this product

Anti-Rab7 (41 citations) Rab7 (D95F2) (6 citations) Rabbit anti-Rab7 (D95F2; (2 citations)

4 protocols using anti rab7 d95f2

Endosomal Trafficking Lipid Composition

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DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), bovine liver phosphatidylinositol (PI), brain PS, and egg PA were purchased from Avanti Polar Lipids. PI 3-phosphate diC16 [PI(3)P], PI 4-phosphate diC16 [PI(4)P], and PI 5-phosphate diC16 [PI(5)P] were obtained from Echelon Biosciences. Folch fraction I and egg PC were purchased from Sigma-Aldrich. YM201636 PIKfyve inhibitor was purchased from Cayman Chemical. A cell fractionation kit was obtained from Cell Signaling Technology. Nontargeting control siRNA was obtained from Horizon Discovery, and human KLC1/2 siRNAs were from Santa Cruz Biotechnology. The following primary antibodies were used: horseradish peroxidase (HRP)–conjugated anti-His⁶ (71841, Novagen), anti-GFP for immunoblotting (3E1, Roche), anti-actin (AC-74, Sigma-Aldrich), anti–β-tubulin (AA2, Sigma-Aldrich), anti–histone H3 (Abcam), anti-giantin (PRB-114C, Covance), anti-HA (HA-7, Sigma-Aldrich), anti-KLC1 ([EPR12441(B)], Abcam), anti-KLC2 (Abcam), anti-KIF5B (Abcam), anti-LAMP1 (lysosome-associated membrane protein 1) (D2D11, Cell Signaling Technology), anti-Rab7 (D95F2, Cell Signaling Technology), and anti-Rab6 (D37C7, Cell Signaling Technology). Alexa 568– and Alexa 633–conjugated anti-mouse or anti-rabbit secondary antibodies were from Thermo Fisher Scientific.

Antón Z., Weijman J.F., Williams C., Moody E.R., Mantell J., Yip Y.Y., Cross J.A., Williams T.A., Steiner R.A., Crump M.P., Woolfson D.N, & Dodding M.P. (2021). Molecular mechanism for kinesin-1 direct membrane recognition. Science Advances, 7(31), eabg6636.

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Antibody Validation for Western Blotting

Cited 1 time

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Primary antibodies used for Western blotting and immunofluorescent staining were as follows: anti–syntaxin 7 (AF5478; R&D Systems), VAMP8 (104302; Synaptic Systems, Goettingen, Germany), MPO (HM1051; Hycult Biotech, Uden, Netherlands), TLR9 (PA5-20203; Thermo Fisher Scientific, Asheville, NC), phosphorylated Erk and total Erk (9101, 9202; Cell Signaling Technology, Danvers, MA), phosphorylated P38 and total P38 (9211, 9212; Cell Signaling Technology), Flag (CGAB-DDK; Genecopoeia), GFP (A6455; Life Technologies, Carlsbad, CA), LAMP1 (sc-19992; Santa Cruz Biotechnology, Santa Cruz, CA), Myc (sc-40; Santa Cruz Biotechnology), Munc13-4 (described previously; Brzezinska et al., 2008 (link)), and anti-Rab7 (D95F2; Cell Signaling Technology).

He J., Johnson J.L., Monfregola J., Ramadass M., Pestonjamasp K., Napolitano G., Zhang J, & Catz S.D. (2016). Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses. Molecular Biology of the Cell, 27(3), 572-587.

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Immunofluorescence Imaging of Endosomal Markers

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Cells were grown to 70% confluence on glass coverslips, fixed for 20 minutes at room temperature in 4% paraformaldehyde, and blocked in 50mM ammonium chloride for 10 minutes and subsequently 0.1% bovine serum albumin in phosphate-buffered saline (PBS) for 20 minutes. Cells were incubated with primary antibodies anti-BMP/LBPA antibody clone 6C4 (MABT837, Millipore Sigma; 1/100), anti-LAMP1 (L1418, Sigma-Aldrich; 1:100) and anti-Rab7 D95F2 (9367, Cell Signaling; 1/100) in PBS containing 0.5% saponin for 30 minutes at room temperature. Following incubation with Alexa Fluor secondary antibodies (1:1000) for 30 minutes, cells were mounted in 4′,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (0100–20, Southern Biotech). Samples were imaged with a Zeiss LSM 710 AxioObserver confocal microscope and analyzed with ZenBlue software.

Butler D.L., Jakielaszek C.J., Miller L.A, & Poupard J.A. (1999). Escherichia coli ATCC 35218 as a Quality Control Isolate for Susceptibility Testing of Haemophilus influenzae with Haemophilus Test Medium. Antimicrobial Agents and Chemotherapy, 43(2), 283-286.

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Immunofluorescent Characterization of Endocytic Organelles

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Cells were grown to 70% confluence on glass coverslips, fixed for 20 minutes at room temperature in 4% paraformaldehyde, and blocked in 50mM ammonium chloride for 10 minutes and subsequently 0.1% bovine serum albumin in phosphate-buffered saline (PBS) for 20 minutes. Cells were incubated with primary antibodies anti-BMP/LBPA antibody clone 6C4 (MABT837, Millipore Sigma; 1/100), anti-LAMP1 (L1418, Sigma-Aldrich; 1:100) and anti-Rab7 D95F2 (9367, Cell Signaling; 1/100) in PBS containing 0.5% saponin for 30 minutes at room temperature. Following incubation with Alexa Fluor secondary antibodies (1:1000) for 30 minutes, cells were mounted in 4′,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (0100-20, Southern Biotech). Samples were imaged with a Zeiss LSM 710 AxioObserver confocal microscope and analyzed with ZenBlue software.

Berg A.L., Showalter M.R., Kosaisawe N., Hu M., Stephens N.C., Sa M., Heil H., Castro N., Chen J.J., VanderVorst K., Wheeler M.R., Rabow Z., Cajka T., Albeck J., Fiehn O, & Carraway KL 3.r.d. (2023). Cellular transformation promotes the incorporation of docosahexaenoic acid into the endolysosome-specific lipid bis(monoacylglycerol)phosphate in breast cancer. Cancer letters, 557.

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