Serum GDF-15 level was measured by a Human Quantikine ELISA Kit (DGD150 for GDF-15, R&D Systems, Minneapolis, MN, USA). Samples, reagents, and buffers were prepared according to the manufacturers’ manuals. The detection threshold of GDF-15 was 2.0 pg/mL. The serum concentrations of TNFα were measured by a Human Quantikine HS ELISA Kit (HSTA00E, R&D Systems, Minneapolis, MN, USA), and the detection threshold was 0.022 pg/mL. The serum IGF-1 concentration was measured by a Human Quantikine ELISA Kit (DG100, R&D Systems), and the detection threshold was 0.026 ng/mL.
Human quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States, United Kingdom
The Human Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human analytes in cell culture supernates, serum, plasma, and other biological fluids.
Automatically generated - may contain errors
Lab products found in correlation
Human quantikine hs elisa kit, by R&D Systems (2 mentions)
Gdf 8 myostatin quantikine elisa kit, by R&D Systems (2 mentions)
Human eia kit, by Phoenix Pharmaceuticals (2 mentions)
Human elisa kit, by Thermo Fisher Scientific (2 mentions)
Neuroinflammation panel 1 human kit, by Mesoscale (2 mentions)
Human rat aβ elisa kit, by Fujifilm (2 mentions)
Synergy 2 elisa plate reader, by Agilent Technologies (2 mentions)
Human xl cyt disc premixed mag luminex perf assay kit, by R&D Systems (2 mentions)
Nadp nadph assay, by Abcam (1 mentions)
Phrodo green e coli bioparticles conjugate for phagocytosis, by Thermo Fisher Scientific (1 mentions)
Labsystems multiskan ms, by Thermo Fisher Scientific (1 mentions)
Elisa kit, by Cusabio (1 mentions)
Endocab igm, by Hycult Biotech (1 mentions)
Versamax microplate reader, by Molecular Devices (1 mentions)
Mouse elisa kit, by Abcam (1 mentions)
Human total adiponectin acrp30 quantikine elisa kit, by R&D Systems (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Mirneasy mini kit, by Qiagen (1 mentions)
Mirneasy serum plasma kit, by Qiagen (1 mentions)
Meso quickplex sq 120, by Mesoscale (1 mentions)
92 protocols using human quantikine elisa kit
1
Serum Biomarker Assessment Protocol
2
Vascular Biomarkers in Fasting Blood Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All of the blood samples were obtained after an overnight fasting. The vascular inflammation, atherosclerosis, and angiogenesis related biomarkers including high-sensitive C-reactive protein (hs-CRP), total nitric oxide, interleukin-10, tumor necrosis factor-α, stromal cell-derived factor-1α (SDF-1α), and VEGF were analyzed by Quantikine human ELISA kits (R&D systems, Minneapolis, MN, USA). The serum level of asymmetric dimethylarginine (ADMA) was also measured by human ELISA kit (DLD Diagnostika GmbH, Hamburg, Germany). All the measurements were conducted according to the standard methods in the operation mannul.
3
Serum Biomarker Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Up to 4 ml of blood samples were obtained in BD (Becton Dickinson Diagnostics, NJ, USA) vacutainers. The blood was stored at room temperature for 45 min, after which the serum was separated by centrifugation at 1500xg, divided into aliquots, snap frozen and stored at -80°C until assay. The serum levels of each component were measured using commercially available Quantikine human ELISA kits: R&D systems, Minneapolis, MN, USA for AdipoQ, IGF-1, ACE and ALPCO Diagnostics, Salem, NH for CETP. The tests were performed according to the manufacturer’s specifications for each ELISA kit. The sensitivity of the assay for AdipoQ was 0.246 ng/mL, for IGF-1 0.026 ng/mL, for ACE 0.019 ng/mL and for CETP 0.2 μg/mL. The assay range for AdipoQ was 0.9-21.4 μg/mL, for IGF-1 40-258 ng/mL, for ACE 37.2-202 ng/mL and for CETP 0.2-5.0 μg/mL.
4
Plasma Ang-2 and sTie-2 Quantification
5
ELISA quantification of IL-6 and TGF-β1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IL-6 and TGF-β1 levels were measured using Quantikine human ELISA kits from R&D Systems (Minneapolis, MN, USA), according to the manufacturer’s instructions. Levels of IL-6 and TGF-β1 proteins in the culture supernatants were expressed as fold increases, normalized by the total proteins. Each test was performed in triplicate.
6
Quantifying Angiogenic and Inflammatory Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NLECs, TLECs, and NLECs co-cultured with OCUM12 were incubated for 3 days in growth factor-free EBM2; supernatants from individual cultures were collected, centrifuged, and filtered through nylon mesh. Quantikine human ELISA Kits (R&D Systems) were used according to the manufacturer's instructions to determine the concentrations of VEGF, VEGF-C, and inflammatory cytokine IL-1β in culture supernatants.
7
Assessing Cellular Metabolism and Immunity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was assessed using Trypan blue and counted using a hemocytometer. Intracellular reduced and oxidized glutathione levels were measured and normalized per 1 million cells as previously described (33 (link)). NADP and NADPH levels were measured using an NADP/NADPH Assay (Abcam) and normalized by cell count. Nitric oxide production was assessed by a Griess assay on the culture supernatant (34 (link)). Phagocytic capacity of monocytes was assessed by exposing the monocytes to the pHrodo Green E. coli BioParticles Conjugate for Phagocytosis (ThermoFisher Scientific) for 30 minutes and the endpoint signal was measured using a spectrophotometer at OD 495nm. IL-6, IL-8, and IL10 levels were measured in culture supernatant using Quantikine Human ELISA kits (R&D Systems).
8
Plasma sTNFR2 Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma levels of sTNFR2 were determined with Quantikine human ELISA kits from R&D Systems. According to the manufacturer’s instructions in the ELISA kits, the intra-assay and inter-assay CVs for the sTNFR2 ELISAs, as measured in 20 plasma samples, averaged 3.5% and 4.1%, respectively. The minimum detectable dose of human sTNF2 was 0.6 pg/mL, and all values obtained from our plasma samples were above the minimum detectable dose. All plasma samples were sent to Chiba University, and the plasma levels of sTNFR2 were measured in Chiba University.
9
Plasma Ang-2 and sTie-2 Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Circulating levels of plasma Ang-2 and sTie-2 were assayed using commercially available Quantikine human ELISA kits (R&D systems). The intra-and inter-assay coefficients of variation were 6.5% and 9.1%, respectively, for Ang-2 and 4.4% and 6.5%, respectively, for Tie-2. The assays were performed according to the manufacturer’s instructions.
10
Plasma MMP-9 Level Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood samples were collected from each subject using VENOJECT Ⅱ vacuum blood collection tube, EDTA‐2Na, 7 mL (TERUMO). The samples were centrifuged, and the plasma was preserved at −80℃ until further testing. Plasma levels of MMP‐9 were determined with Quantikine human ELISA kits from R&D Systems. According to the manufacturer's instructions in the ELISA kits, the intra‐assay and interassay coefficients of variation for the MMP‐9 ELISAs, as measured in 20 plasma samples, averaged 2.3% and 7.5%, respectively. The minimum detectable dose of human MMP‐9 was 0.156 ng/mL, and all values obtained from our plasma samples were above the minimum detectable dose.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!