Dulbecco s modified eagles s medium

Rat cardiomyoblast cell line, H9c2, was obtained from American Type Culture Collection (ATCC, Manassas, VA) and maintained in culture using Dulbecco's Modified Eagles's Medium (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/ml penicillin, 100 mg/ml streptomycin). Cells were grown at 37°C in a humidified 95% air/5% CO₂ atmosphere.

Cord blood was obtained from the Clinic of Gynecology of the Medical University of Vienna. All donors gave their written informed consent. CD34⁺ hematopoietic stem cells were isolated from cord blood with CD34 MACS magnetic beads (Miltenyi, Bergisch Gladbach, Germany). CD34⁺ cells were cultured with 50 ng mL⁻¹ thrombopoietin, 1 ng mL⁻¹ stem cell factor and interleukin‐3 (Miltenyi) in Stem Pro 34 medium (Invitrogen, Carlsbad, CA, USA) at 37 °C in 5% CO₂ for 12 days to obtain mature culture differentiated megakaryocytes. Mature megakaryocytes were stained with CD41, CD14 and CD45 antibodies (eBioscience, San Diego, CA, USA), and their purity was analyzed by fluorescence‐activated cell sorting (FACS). Cultures containing > 3% contaminating leukocytes were used for our experiments. Cell lines CHRF, Meg‐01, HL‐60 and HepG2 were purchased from ATCC. They were grown in RPMI medium or Dulbecco's modified Eagles's medium (Invitrogen) supplemented with 10% fetal bovine serum and gentamicin (Gibco, Carlsbad, CA, USA) at 37 °C in 5% CO₂.

CAL27 and CAL33 HNSCC cell lines^{13 (link)} were kindly provided by Dr J.L. Fischel (Centre Antoine Lacassagne, Nice, France, where they were initially obtained). They were authenticated by Azur Génétique (Nice, France) as identical to the corresponding DSMZ cell lines (report reference AGLC-14-00225). They were cultured in Dulbecco’s modified Eagles’s medium (Invitrogen, Cergy-Pontoise, France) supplemented with 10% heat-inactivated foetal calf serum (Pan Biotech) and maintained at 37 °C in a humidified atmosphere containing 5% CO₂ in Petri dishes or flasks of various sizes. They were replicated every 4–5 days, and the medium was changed once in-between.