Tumors were enzymatically digested with collagenase type I (1.6 kU/mL; Sigma) St. Louis, MO, USA and DNase type I (1.5 kU/mL; Sigma). Digested tumor suspension was stained with PE-conjugated anti-CD11b antibody (BD Bioscience), PE-conjugated anti-F4/80 antibody (AbD Serotech), or PE-conjugated anti-CD31 antibody (BioLegend), and analyzed by using a FACS Aria II system (BD Bioscience). Cells were gated according to forward/side scatter, and dead cells were excluded by using propidium iodide (Sigma) staining. In some experiments, cells were sorted from the mixture of all the tumors in each group. The whole RNA was extracted, and cDNA was obtained by using a Cells-to-CT kit (Applied Biosystems) Foster City, CA, USA for quantitative PCR analysis.
Pe conjugated anti cd31 antibody
Manufactured by BioLegend
Sourced in United States
PE-conjugated anti-CD31 antibody is a reagent used for the detection and analysis of CD31 (also known as PECAM-1) expression on cells by flow cytometry. CD31 is a cell surface glycoprotein that is expressed on endothelial cells, platelets, and some leukocyte subsets.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase type 1, by Merck Group (1 mentions)
Dnase type 1, by Merck Group (1 mentions)
Cells to ct kit, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Facsaria 2 system, by BD (1 mentions)
Pe conjugated anti cd11b antibody, by BD (1 mentions)
Facsaria 3, by BD (1 mentions)
7 aminoactinomycin d, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
2 protocols using pe conjugated anti cd31 antibody
1
Tumor Cell Isolation and Analysis
2
Flow Cytometric Analysis of Endothelial Cells
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Cells were harvested using 0.25% trypsin–EDTA (Invitrogen, CA, USA) and then resuspended in PBS/2% FBS at a density of 1 × 106 cells per 100 μL. The cells were incubated for 30 min with PE conjugated anti CD31 antibody (BioLegend, CA, USA) or Alexa fluor 647 conjugated anti CD144 antibody (BioLegend, CA, USA) and dead cells were excluded by staining with 7-aminoactinomycin D (Invitrogen, CA, USA). Analyses were performed using a FACS Aria III (BD Bioscience, NJ, USA) and data were analyzed using FlowJo software Version 10 (FlowJo, OR, USA).
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