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Model 1007d

Manufactured by Durect
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The Model 1007D is a laboratory equipment designed for general-purpose use. It features a compact and durable construction, providing a reliable tool for various laboratory applications. The core function of this model is to assist in the performance of standard laboratory procedures and experiments.

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Lab products found in correlation

U46619, by Cayman Chemical (2 mentions) Metabolic cages, by AccuScan Instruments (1 mentions) Alzet osmotic pump, by Durect (1 mentions) Osmotic pump, by Durect (1 mentions) Radiotelemeters, by Data Sciences International (1 mentions) Ara c, by Merck Group (1 mentions) Brain infusion kit 3, by Durect (1 mentions)

9 protocols using model 1007d

1

Leptin Metabolic Effects Mediated by IRS2

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In separate experiments, IRS2flox/flox (n=9), Nestin-cre (n=4), IRS2/POMC-cre (n=7) and IRS2/Nestin-cre mice (n=7) at 22 weeks of age were placed individually in metabolic cages (AccuScan Instruments Inc, Columbus, OH) equipped with oxygen sensors to measure oxygen consumption (VO2) and motor activity. VO2 was measured for 2-min at 10-minute intervals continuously 24-hours a day using a Zirconia oxygen sensor. Motor activity was determined using infrared light beams mounted in the cages in X, Y and Z axes. After the mice were acclimatized to the new environment for approximately 4 to 6 days, VO2 and motor activity were recorded for 3 consecutive days. Then, the mice were lightly anesthetized with isoflurane and an osmotic minipump (model 1007D, Durect Corp.) was placed intraperitoneally to deliver leptin (4 µg/kg/min) for 7 days. Mice were followed for an additional 5-day post-treatment period.
To further investigate the role of IRS2 signaling on glucose homeostasis and to test the hypothesis that leptin-mediated activation of IRS2 plays an important role in mediating the chronic antidiabetic actions of leptin, we performed additional chronic leptin infusion experiments in STZ-diabetic IRS2/Nestin-cre and IRS2flox/flox mice.
do Carmo J.M., da Silva A.A., Wang Z., Freeman N.J., Alsheik A.J., Adi A, & Hall J.E. (2015). REGULATION OF BLOOD PRESSURE, APPETITE AND GLUCOSE BY LEPTIN AFTER INACTIVATION OF INSULIN RECEPTOR SUBSTRATE 2 (IRS2) SIGNALING IN THE ENTIRE BRAIN OR IN POMC NEURONS. Hypertension, 67(2), 378-386.
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2

Subcutaneous Alzet Pump Delivery of GNE-R-140

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A subcutaneous Alzet pump (Model 1007D, Durect Corporation, Cupertino, CA, USA) with an infusion rate 0.5 µL/h and an infusion duration of 7 days was implanted on the back mice, following the MSKCC IACUC protocol. Two groups of mice (treated and control) were established, and both groups were implanted with the Alzet pumps. In the treated group of mice, the Alzet pumps were loaded with 18 mg GNE-R-140 in 100 µL of 100% DMSO, delivering 100 mg/kg/day to ensure a blood concentration of 10 mM over the duration of the experiment. In the control group, the Alzet pumps were loaded with DMSO only.
Maeda M., Ko M., Mane M.M., Cohen I.J., Shindo M., Vemuri K., Serganova I, & Blasberg R. (2022). Genetic and Drug Inhibition of LDH-A: Effects on Murine Gliomas. Cancers, 14(9), 2306.
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3

Central Kappa-Opioid Receptor Antagonism and Metabolic Outcomes

Cited 2 times
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To assess the chronic central effects of the k-OR selective antagonist, PF-04455242, WT, and Oprk1−/−animals were subjected to OVX 21 days after the surgery PF-04455242 (3.4 nmol/day) or vehicle (VH) were delivered i.c.v. via brain infusion kit 3 (1–3 mm) for 7 days through a catheter tube connecting the i.c.v. cannula to an osmotic minipump (model 1007D, Alzet Osmotic Pumps; DURECT, Cupertino, CA, USA), as previously described [43 (link),46 (link),50 (link)]. The mini pump was inserted in a subcutaneous pocket on the dorsal surface. The incision was closed with sutures, and mice were kept warm until full recovery. Body weight, food intake, lipid metabolism, and thermogenesis/browning were analyzed.
Romero-Picó A., Novelle M.G., Al-Massadi O., Beiroa D., Tojo M., Heras V., Ruiz-Pino F., Senra A., López M., Blouet C., Tena-Sempere M., Nogueiras R, & Diéguez C. (2022). Kappa-Opioid Receptor Blockade Ameliorates Obesity Caused by Estrogen Withdrawal via Promotion of Energy Expenditure through mTOR Pathway. International Journal of Molecular Sciences, 23(6), 3118.
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4

Modeling IUGR and OIR via Gestational TXA2 Infusion

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To model HDP and UPI with resultant IUGR in offspring, we infused a thromboxane A2-analog (TXA2, U-46619) instilled into micro-osmotic pumps in the last week of the mouse gestation as previously described.21 (link) On embryonic day 12.5, heterozygous (hWtEPOR/ mWtEpoR) dams were anesthetized by intraperitoneal injection of ketamine (40 μg/g) and xylazine (8 μg/g). Micro-osmotic pumps (model 1007D, 0.5 μl/h, Durect Corporation, Cupertino, CA) containing either vehicle (0.5% ethanol) or 2000 ng/h of U-46619 (TXA2 analog, catalog no. 16450, Cayman Chemical, Ann Arbor, MI) were then placed into the retroperitoneum through a 1 cm incision. Dams with TXA2 infusion developed hypertension within 24 h of implantation. Implants remained for the remainder of gestation, which is ~20 days in C57Bl/6J mice. Upon birth, litters from implanted heterozygous dams were cross-fostered to non-implanted dams to reduce potential confounding effects from surgery. During our model inception,21 (link) we showed that fetal 11-dehydrothromoboxane B2 level, a stable TXA2 metabolite, was no different in pups born to dams receiving sham or U-46619 infusion, therefore the analog did not cross the placenta to affect the pups directly. As such, we do not believe that a direct interaction between TXA2 and EPO would occur in this postnatal model of IUGR and OIR.
Glerum D.M, & Tzagoloff A. (1994). Isolation of a human cDNA for heme A:farnesyltransferase by functional complementation of a yeast cox10 mutant.. Proceedings of the National Academy of Sciences of the United States of America, 91(18), 8452-8456.
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5

Modeling IUGR via TXA2 analog infusion

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To model HDP and UPI with resultant IUGR in offspring, we infused a thromboxane A2-analog (TXA2, U-46619) instilled into micro-osmotic pumps in the last week of the mouse gestation as previously described.21 (link) On embryonic day 12.5, heterozygous (hWtEPOR/ mWtEpoR) dams were anesthetized by intraperitoneal injection of ketamine (40 μg/g) and xylazine (8 μg/g). Micro-osmotic pumps (model 1007D, 0.5 μl/h, Durect Corporation, Cupertino, CA) containing either vehicle (0.5% ethanol) or 2000 ng/h of U-46619 (TXA2 analog, catalog no. 16450, Cayman Chemical, Ann Arbor, MI) were then placed into the retroperitoneum through a 1 cm incision. Dams with TXA2 infusion developed hypertension within 24 h of implantation. Implants remained for the remainder of gestation, which is ~20 days in C57Bl/6J mice. Upon birth, litters from implanted heterozygous dams were cross-fostered to non-implanted dams to reduce potential confounding effects from surgery. During our model inception,21 (link) we showed that fetal 11-dehydrothromoboxane B2 level, a stable TXA2 metabolite, was no different in pups born to dams receiving sham or U-46619 infusion, therefore the analog did not cross the placenta to affect the pups directly. As such, we do not believe that a direct interaction between TXA2 and EPO would occur in this postnatal model of IUGR and OIR.
Fung C., Cung T., Nelson C., Wang H., Bretz C., Ramshekar A., Brown A., Stoddard G.J, & Hartnett M.E. (2023). Retinopathy of prematurity protection conferred by uteroplacental insufficiency through erythropoietin signaling in an experimental Murine Model. Pediatric Research, 94(3), 950-955.
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6

Long-term Effects of IL18 on Food Intake, Body Composition, and iWAT

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To assess the long-term central effects of the IL18 system on food intake, body composition and iWAT morphology, 10–11 weeks old Il18r1-KO and WT mice (4–7 mice/genotype/treatment) were infused ICV with saline or recombinant IL18 (20 μg/mouse/7 days, Sino Biological, Inc., Beijing, China)56 (link). For this purpose, brain infusion canulae were placed stereotaxically into the lateral ventricle as previously described56 (link). A catheter tube was connected from the brain infusion cannula to the osmotic minipump (model 1007D, total volume 100 μL, flow rate 0.5 μL/h; Alzet Osmotic Pumps, Durect, CA, USA) flow moderator.
Pazos P., Lima L., Tovar S., González-Touceda D., Diéguez C, & García M.C. (2015). Divergent responses to thermogenic stimuli in BAT and subcutaneous adipose tissue from interleukin 18 and interleukin 18 receptor 1-deficient mice. Scientific Reports, 5, 17977.
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7

Peptide Therapy for Post-MI Cardiac Function

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A 24 amino acid peptide (YQPPSTNKNTKSQRRKGSTFEEHK), corresponding to the unique C-terminal E-domain of the human MGF isoform, was synthesized and purified to >90% via HPLC (Genescript Corp, NJ). The peptide was stabilized by amidating the C-terminus and switching the arginines at positions 14 and 15 to the D-stereoisomer. Micro-osmotic pumps (Alzet, Model 1007D, Durect Corp, CA) were loaded with vehicle (saline) or peptide dissolved in vehicle to allow an infusion rate of 4.5 mg/Kg/day for 2 weeks. Pumps were implanted subcutaneously in anesthetized mice through a small incision between the scapulae [8 (link)]. Mice were randomized following MI into three groups. In one group, pumps containing peptide were implanted immediately following MI (acute phase) and removed after 2 weeks. In the second group, pumps containing peptide were implanted at 8 weeks post-MI (chronic phase). The third group pumps containing saline were implanted immediately following MI (acute phase) and removed after 2 weeks. Cardiac function was analyzed in all groups of mice at 10 weeks post-MI.
Shioura K., Pena J, & Goldspink P. (2014). Administration of a Synthetic Peptide Derived from the E-domain Region of Mechano-Growth Factor Delays Decompensation Following Myocardial Infarction. International journal of cardiovascular research, 3(3), 1000169-.
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8

Telemetric Blood Pressure Monitoring in Mice

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HR, systolic blood pressure (SBP), MAP and diastolic blood pressure (DBP) were measured in conscious, freely moving mice using radiotelemeters (Data Sciences International), as we previously described [32 (link)]. Daily recordings (1 every 4 min, 360 recordings/mouse per day) started after complete recovery from surgery (1–2 weeks) and were carried on continuously for 4 days (baseline). Angiotension II (Ang II, 1mg/kg body weight/day) was then administered via osmotic minipumps (model 1007D, Alzet, Durect Corporation, Cupertino, CA, USA) for the following 7 days. The average of 360 daily recordings for each mouse was calculated before and after Ang II.
Tong X., Khandelwal A.R., Wu X., Xu Z., Yu W., Chen C., Zhao W., Yang J., Qin Z., Weisbrod R.M., Seta F., Ago T., Lee K.S., Hammock B.D., Sadoshima J., Cohen R.A, & Zeng C. (2016). Pro-atherogenic Role of Smooth Muscle Nox4-based NADPH oxidase. Journal of molecular and cellular cardiology, 92, 30-40.
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9

Intracerebroventricular Infusion of Ara-C

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ICV infusions were performed using, 7-d osmotic pumps (Alzet, model 1007D; Durect) attached to brain infusion cannula (Alzet, Brain infusion kit 3; Durect). Cannulae were stereotaxically implanted in the right ventricle at coordinates: 0 mm AP and −0.9 mm ML to the bregma. For antimitotic experiments, 2% of Ara-C (Sigma-Aldrich) or vehicle was infused for 7 d, and then animals were either euthanized for immediate analysis or pumps were removed and mice euthanized 21 d later.
Joppé S.E., Cochard L.M., Levros LC J.r., Hamilton L.K., Ameslon P., Aumont A., Barnabé-Heider F, & Fernandes K.J. (2020). Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium. Life Science Alliance, 3(7), e202000743.
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