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Mouse anti hnk1

Manufactured by Developmental Studies Hybridoma Bank
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Mouse anti-HNK1 is an antibody product developed and distributed by the Developmental Studies Hybridoma Bank. It is a mouse-derived monoclonal antibody that specifically recognizes the HNK-1 carbohydrate antigen.

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Lab products found in correlation

Alexa dye, by Thermo Fisher Scientific (2 mentions) Mouse anti hu huc d, by Thermo Fisher Scientific (2 mentions) Axiocam 503 camera, by Zeiss (1 mentions) Zen 2.3 blue edition software, by Zeiss (1 mentions) Mouse anti tuj1, by Fortrea (1 mentions) Axio observer 7 inverted microscope, by Zeiss (1 mentions)

3 protocols using mouse anti hnk1

1

Tracing Virus Spread in Zebrafish Retina

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Prior to injection, larval zebrafish were anesthetized with 0.01% tricaine, and immobilized in 1.5% low melting‐point agarose. 1–2 nL of rVSV‐Venus(VSV‐G), 1 × 106 or 1 × 108 ffu/mL, rVSV‐GFP(RABV‐G), 1 × 108 ffu/mL, or rVSVΔG‐GFP(RABV‐G), 1 × 108, or 1 × 109 ffu/mL was injected into the vitreal cavity of one eye. Infected animals were fixed at 24, 48, or 72 hpi and processed by whole‐mount immunohistochemistry. Samples were incubated with rabbit anti‐GFP to detect Venus or GFP (MBL International, Woburn, MA; 598, RRID:AB_591819), mouse anti‐HU (HuC/D) (Life Technologies, Bethesda, MD; RRID:AB_591819), and mouse anti‐HNK1 (Developmental Studies Hybridoma Bank, Iowa City, IA; RRID:AB_531908) followed by secondary antibodies conjugated with Alexa dyes (Life Technologies, 1:500). Stained samples were mounted in 1.5% low‐melting‐point agarose and imaged with confocal microscopy. 3D rendering of zebrafish larvae was generated with FluidVis (Fluidity Software, Somerville, MA), followed by manual annotation in ImageJ (NIH, Bethesda, MD; RRID:nif‐0000‐30467) and Photoshop.
Mundell N.A., Beier K.T., Pan Y.A., Lapan S.W., Göz Aytürk D., Berezovskii V.K., Wark A.R., Drokhlyansky E., Bielecki J., Born R.T., Schier A.F, & Cepko C.L. (2015). Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms. The Journal of Comparative Neurology, 523(11), 1639-1663.
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2

Immunolabeling of Whole Embryos

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Whole embryos were fixed for 15 min in 4% PFA, washed in TBST (500 mM Tris-HCl, pH 7.4, 1.5 M NaCl, 10 mM CaCl2, and 0.5% Triton X-100) and blocked in 5% FBS in TBST for 1h at RT. Embryos were then incubated in mouse anti-Tuj1 (1:250; Covance) and/or mouse anti-HNK1 (1:10; supplied by the Developmental Studies Hybridoma Bank) overnight at 4°C diluted in TBST-FBS. Secondary antibodies were goat anti-mouse IgG2a Alexa Fluor 647 (1:500) and goat anti-mouse IgM Alexa Fluor 568 (1:500) for one hour at room temperature. After several washes in TBS-T, whole embryos or sections were mounted and imaged by using Carl ZEISS Axio observer 7 inverted microscope containing an Axiocam 503 camera and Carl ZEISS ZEN2.3 (blue edition) software.
Bernardi Y.E., Sanchez-Vasquez E., Piacentino M.L., Urrutia H., Rossi I., Saraiva K.L., Pereira-Neves A., Ramirez M.I., Bronner M.E., de Miguel N, & Strobl-Mazzulla P.H. (2023). Extracellular vesicle-localized miR-203 mediates neural crest-placode communication required for trigeminal ganglia formation. bioRxiv.
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3

Viral Tracing in Larval Zebrafish

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Prior to injection, larval zebrafish were anesthetized with 0.01% tricaine, and immobilized in 1.5% low melting-point agarose. 1–2 nL of rVSV-Venus(VSV-G), 1 × 106 or 1 × 108 ffu/mL, rVSV-GFP(RABV-G), 1 × 108 ffu/mL, or rVSVΔG-GFP(RABV-G), 1 × 108, or 1 × 109 ffu/mL was injected into the vitreal cavity of one eye. Infected animals were fixed at 24, 48, or 72 hpi and processed by whole-mount immunohistochemistry. Samples were incubated with rabbit anti-GFP to detect Venus or GFP (MBL International, Woburn, MA; 598, RRID:AB_591819), mouse anti-HU (HuC/D) (Life Technologies, Bethesda, MD; RRID:AB_591819), and mouse anti-HNK1 (Developmental Studies Hybridoma Bank, Iowa City, IA; RRID:AB_531908) followed by secondary antibodies conjugated with Alexa dyes (Life Technologies, 1:500). Stained samples were mounted in 1.5% low-melting-point agarose and imaged with confocal microscopy. 3D rendering of zebrafish larvae was generated with FluidVis (Fluidity Software, Somerville, MA), followed by manual annotation in ImageJ (NIH, Bethesda, MD; RRID:nif-0000-30467) and Photoshop.
Mundell N.A., Beier K.T., Pan Y.A., Lapan S.W., Göz Aytürk D., Berezovskii V.K., Wark A.R., Drokhlyansky E., Bielecki J., Born R.T., Schier A.F, & Cepko C.L. (2015). Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms. The Journal of Comparative Neurology, 523(11), 1639-1663.
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