The CCK-8 assay (Dojindo Molecular Technologies, Inc.) was performed to test cell viability. Following treatment with 8 nM paclitaxel (APeXBIO Technology LLC), 20 ng/ml recombinant human IL-1β (Sangon Biotech Co., Ltd.), 0.1 µg/ml tunicamycin (Beijing Solarbio Science & Technology Co., Ltd.), paclitaxel + IL-1β, paclitaxel + tunicamycin and blank control, then 2x103 A549 cells were seeded into 96-well plates and incubated at 37˚C with 5% CO2 for 48 h. At 0, 12, 24 and 48 h, 10 µl CCK-8 reagent was added to each well and incubated with the cells for 2 h. Absorbance was measured at 450 nm by a microplate reader (Thermo Fisher Scientific, Inc.).
Recombinant human il 1β
Manufactured by Sangon
Sourced in China
Recombinant human IL-1β is a cytokine produced through recombinant DNA technology. It is a key mediator of the inflammatory response and is involved in a variety of cellular activities.
Automatically generated - may contain errors
Lab products found in correlation
Tunicamycin, by Solarbio (1 mentions)
Il 1β, by Solarbio (1 mentions)
Microplate reader, by Thermo Fisher Scientific (1 mentions)
Cck 8 assay, by Dojindo Laboratories (1 mentions)
Paclitaxel, by Thermo Fisher Scientific (1 mentions)
Tunicamycin, by Thermo Fisher Scientific (1 mentions)
Paclitaxel, by Solarbio (1 mentions)
Paclitaxel, by Apexbio (1 mentions)
Gapdh mouse monoclonal antibody, by ABclonal (1 mentions)
E cadherin antibody, by Cell Signaling Technology (1 mentions)
N cadherin antibody, by Cell Signaling Technology (1 mentions)
Vimentin antibody, by Cell Signaling Technology (1 mentions)
Cox 2 antibody, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
2 protocols using recombinant human il 1β
1
Cell Viability Evaluation by CCK-8 Assay
2
Reagents and Antibodies for Cell Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DMEM/F12 medium (#SH30023.01) was from Hyclone (Shanghai, China) and fetal bovine serum (FBS; #04–001-1A) was bought from Biological Industries (BI; Cromwell, CT, USA). 17β-estradiol/estrogen/E2 (#E2758) and lipopolysaccharides (LPS; #L4391) were from Sigma-Aldrich (Shanghai, China). Recombinant Human IL-1β (#C600002) was bought from Sangon Biotech (Shanghai, China). GAPDH mouse monoclonal antibody (#40493) was procured from ABclonal (Boston, USA). Cytokeratin 18 antibodies (#4548), E-cadherin antibodies (#14472), Vimentin antibodies (#5741), N-cadherin antibodies (#13116), Progesterone Receptor (PR) antibodies (#8757S), and ERα antibodies (#13258) were acquired from Cell Signaling Technology (Danvers, MA, USA). HSD17B1 antibodies (#ab51045), TERT antibodies (#ab32020), ERβ antibodies (#ab288), COX-2 antibodies (#ab15191) and CYP17A1 antibodies (#ab125022) were purchased from Abcam (Cambridge, UK).
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