Thy1 egfp m

Manufactured by Jackson ImmunoResearch

Thy1-EGFP-M is a fluorescently labeled antibody reagent produced by Jackson ImmunoResearch. It targets the Thy1 (CD90) cell surface antigen and is conjugated to the Enhanced Green Fluorescent Protein (EGFP) reporter molecule.

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Lab products found in correlation

Tamoxifen, by Merck Group (3 mentions) Wnt1 cre2, by Jackson ImmunoResearch (2 mentions) Teto h2bgfp, by Jackson ImmunoResearch (2 mentions) Ttaflox, by Jackson ImmunoResearch (2 mentions) Corn oil, by Merck Group (1 mentions) Gli1 creert2, by Jackson ImmunoResearch (1 mentions) Thy1 yfp 16, by Jackson ImmunoResearch (1 mentions) Shh creert2, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions)

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Thy1-EGFP line M (3 citations)

3 protocols using thy1 egfp m

Transgenic Mouse Lines for Brain Imaging

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For PEGASOS-cleared mouse: Following mice were purchased from the Jackson Lab with genotypes including Thy1-EGFP-M (JAX# 007788), Ai14 (JAX# 007908), tTA^flox (JAX# 008600), tetO-H2BGFP (JAX# 005104) and Wnt1-Cre2 (JAX# 022501). Tg(Cdh5-CreERT2) mice (Strain NO.T014691) were purchased from GemPharmatech (Nanjing, China). R26-mScarlett^flox reporter was generated by Hu Zhao lab in the Chinese Institute for Brain Research. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research. For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/ml. The solution was kept at −20 °C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.
For CUBIC-L/R cleared mouse: A fixed brain of 9-week-old female Thy1-YFP-H Tg mice (B6.Cg-Tg(Thy1-YFP)HJrs/J, The Jackson Laboratory, Identifier: 003782)^{63 (link)} was provided from National Institute for Physiological Sciences (Okazaki, Japan) under the material transfer agreement with Juntendo University. All animal experiments were approved by Juntendo University (1569-2022279 and 1372-2022211), and National Institute for Physiological Sciences (22A044).

Prince M.N., Garcia B., Henn C., Yi Y., Susaki E.A., Watakabe Y., Nemoto T., Lidke K.A., Zhao H., Remiro I.S., Liu S, & Chakraborty T. (2024). Signal improved ultra-fast light-sheet microscope for large tissue imaging. Communications Engineering, 3, 59.

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Genetic Manipulation of Murine Models

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Mice were purchased from the Jackson Lab with genotypes including C57BL/6 (JAX # 000664), Thy1-EGFP-M (JAX# 007788), Thy1-YFP-16 (JAX #003709), Ai14 (JAX# 007908), Ai 140 (JAX# 030220), Shh-Cre^ERT2 (JAX# 005623) and Gli1-cre^ERT2 (JAX# 007913). All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research.
For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/mL. The solution was kept at –20 °C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.

Yi Y., Li Y., Zhang S., Men Y., Wang Y., Jing D., Ding J., Zhu Q., Chen Z., Chen X., Li J.L., Wang Y., Wang J., Peng H., Zhang L., Luo W., Feng J.Q., He Y., Ge W.P, & Zhao H. (2024). Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system. Cell Research, 34(2), 124-139.

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Transgenic Mouse Models for Neural Lineage Tracing

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Following mice were purchased from the Jackson Lab with genotypes including Thy1-EGFP-M (JAX# 007788), Ai14 (JAX# 007908), tTA^flox (JAX# 008600), tetO-H2BGFP (JAX# 005104) and Wnt1-Cre2 (JAX# 022501). Tg(Cdh5-CreERT2) mice (Strain NO.T014691）were purchased from GemPharmatech (Nanjing, China). R26-mScarlett ^flox reporter was generated by Hu Zhao lab in the Chinese Institute for Brain Research. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research. For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/ml. The solution was kept at −20°C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.

Prince M.N., Garcia B., Henn C., Yi Y., Susaki E.A., Watakabe Y., Nemoto T., Lidke K.A., Zhao H., Remiro I.S., Liu S, & Chakraborty T. (2023). Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging. Research Square.

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