The PISCINA II study is an experimental investigation of the acute biological response to exposures induced by swimming in a chlorinated pool. As detailed elsewhere,31 (
link) the study included volunteers, aged 18–40 years, non-smoking and non-professional swimmers, who swam for 40 min in a 25 m long indoor chlorinated pool in Barcelona, Spain, between June and December 2013. At the time of the experiment, participants were asked to complete a questionnaire providing information on sociodemographic, dietary habits, regular physical activity, medical and anthropometric factors.
DBPs including four THMs, CHCl
3, BDCM, DBCM and CHBr
3, were measured in exhaled breath at two time points: before swimmers entered the swimming pool and immediately after they exited the swimming pool, using the
Bio-VOC Sampler (Markes International Ltd, UK). These chemicals were assessed by gas chromatography coupled to a mass spectrometer. Details on sampling collection and analysis have been published previously.31 (
link)
For each of these 60 participants with full exposure and questionnaire data, two blood samples collected before and 2 hours after swimming were available. These were collected in a room detached from the swimming pool area and stored at −80°C.
Informed consent was provided by each participant before commencement of the experiment.
Jain P., Vineis P., Liquet B., Vlaanderen J., Bodinier B., van Veldhoven K., Kogevinas M., Athersuch T.J., Font-Ribera L., Villanueva C.M., Vermeulen R, & Chadeau-Hyam M. (2018). A multivariate approach to investigate the combined biological effects of multiple exposures. Journal of Epidemiology and Community Health, 72(7), 564-571.