Aspartate aminotransferase ast kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Aspartate Aminotransferase (AST) kit is a laboratory reagent used to measure the activity of the AST enzyme in biological samples. AST is an enzyme found in various tissues, and its measurement is commonly used as a biomarker to assess liver function and detect liver damage.

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Aspartate aminotransferase (64 citations) Aspartate aminotransferase (AST) (13 citations) AST kit (9 citations) Aspartate aminotransferase (AST) assay kit (9 citations) Aspartate aminotransferase (AST) microplate test kits (7 citations)

7 protocols using aspartate aminotransferase ast kit

Hepatoprotective Effects of H. candicans

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H candicans was purchased from Ganlu Traditional Medicine (Tibet, China) and was identified by the Shaanxi Chinese Medicine Institute. Bifendate (C₂₀H₁₈O₁₀, DDB) (Cat#H44023176) was purchased from Baiyunshan Xingqun Pharmaceutical Co., Ltd. (Guangzhou, China). Aspartate aminotransferase (AST) kit (Cat# C010-2-1), alanine aminotransferase (ALT) kit (Cat# C009-2-1), superoxide dismutase (SOD) kit (Cat# A001-3-2), and malondialdehyde (MDA) kit (Cat# A003-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). ELISA kits (Rat tumor necrosis factor-alpha (TNFα), Cat# ERA56RB; Rat interleukin 6 (IL-6), Cat# ERA31RBX5) were purchased from Thermo Fisher, USA.

Li J., Song D., Zhang B., Guo J., Li W., Zhang X, & Zhao Q. (2021). Hepatoprotective Effects of Heracleum candicans Against Carbon Tetrachloride-Induced Acute Liver Injury in Rats. Dose-Response, 19(3), 15593258211029510.

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Evaluation of Anticoagulant RIVA's Effects

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Drugs: RIVA, an oral direct inhibitor of factor Xa, was purchased from ChemBest Research Laboratories Ltd. (CAS No. 366789-02-8).
Biochemical Reagents: alanine aminotransferase (ALT) kit (Cat No. C009-1) and aspartate aminotransferase (AST) kit (Cat No. C010-1) were purchased from Nanjing Jiancheng Institute of Biological Engineering. The hydroxyproline standard (trans-4-hydroxy-L-proline, Cat No. H54409) was purchased from Sigma.
Antibodies and pathological testing reagents: SABC Immunohistochemical Staining Kit (Cat No. SA1028-Rabbit IgG), anti-PAI-1 antibody (Cat No. ab66705), anti-fibrinogen rabbit monoclonal antibody (Cat No. ab189490), and anti-von Willebrand factor (vWF)antibody (Cat No. ab6994) were all purchased from Sigma.

Guo Y., Dong S., Li M., Tao Y., Lv J, & Liu C. (2024). A new model of portal vein thrombosis in rats with cirrhosis induced by partial portal vein ligation plus carbon tetrachloride and intervened with rivaroxaban. BMC Gastroenterology, 24, 161.

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DNA Protective Effects in Liver Injury

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Sodium deoxyribonucleic acid salt (derived from salmon sperm) was purchased from Sigma. The mass concentration of DNA in the sodium deoxyribonucleic acid salt of 0.02 mg/mL was determined by NanoDrop to be 1.25 mg DNA/mg sodium deoxyribonucleic acid sodium salt. Sodium carboxymethyl cellulose (0.5%) was used to prepare suspensions containing DNA with final concentrations of 10 mg/kg (low dose), 50 mg/kg (medium dose), and 300 mg/kg (high dose), respectively. Olive oil was purchased from Hangzhou Guanlai Trading Co. Ltd.; CCl₄ was purchased from Sinopharm Chemical Reagent Co. Ltd, diluted with olive oil. Aspartate aminotransferase (AST) kit, alanine aminotransferase (ALT) kit, superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, glutathione peroxidase (GSH‐Px) kit, and glutathione (GSH) kit were all purchased from Nanjing Jiancheng Biological Engineering Co. Ltd, while ELISA kits TNF‐α and IL‐6 were purchased from Thermo Fisher Scientific, USA. TUNEL (terminal deoxynucleotidyl transferase (TdT)‐mediated dUTP nick end labeling) apoptosis kit and bicinchoninic acid (BCA) protein concentration determination kit were purchased from Beijing Solarbio Science & Technology Co. Ltd. β‐Actin antibody was purchased from Affinity and other antibodies were purchased from ProteinTech Group.

Huang X., Chu X., Tian Y., Xue Y., Zhang L., Li J., Hou H., Dong P, & Wang J. (2022). Preventive effect of salmon sperm DNA on acute carbon tetrachloride‐induced liver injury in mice through Nrf2/ARE and mitochondrial apoptosis pathway. Food Science & Nutrition, 11(2), 733-742.

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Antioxidant Biomarkers in ICR Mice

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The animal experiment was approved by the Experimental Animal Care Ethics Committee of Northwest A&F University. A total of 320 ICR mice (male, 9 weeks old) with an average weight of 33 ± 2 g, SPF grade, were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) (Approval Number: SCXK (Beijing) 2012-0001). Before the experiment, all experimental animals were bred adaptively in the animal room for one week. The conditions in the animal room were controlled as follows: the humidity was set to 55% ± 5% and the temperature was set to 25 ± 2 ℃, a 12-h circadian rhythm was ensured, and the experimental animals could drink water and eat freely. After all the mice had adapted to the environment, the experiment was started.
The aspartate aminotransferase (AST) kit, the alanine aminotransferase (ALT) kit, the total bilirubin (TBIL) kit, the alkaline phosphatase (ALP) kit, the total triglyceride (TC) kit, the total cholesterol (TG) kit, the total antioxidant capacity (T-AOC) kit, the catalase (CAT) kit, the glutathione (GSH) kit, the superoxide dismutase (SOD) kit, and the malonaldehyde (MDA) kit were purchased from Nanjing Jiancheng Bioengineering Institute (China).

Sun X., Wang J., Ge Q., Li C., Ma T., Fang Y, & Zhan J. (2022). Interactive Effects of Copper and Functional Substances in Wine on Alcoholic Hepatic Injury in Mice. Foods, 11(16), 2383.

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Quantitative Immunoassay Protocol

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Alanine transaminase (ALT) and aspartate aminotransferase (AST) kits were purchased from Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. (Nanjing, China). Immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA) detection kits were purchased from Shanghai Enzyme Linked Biotechnology Co., Ltd.(Shanghai, China). The primary antibodies used in this study were as follows: anti−p−IκBα (1:1000, bs−2513R, Bioss (Beijng, China)), anti−IκBα (1:1000, bs−1287R, Bioss), β−actin (1:2000, 20536−1−AP, Proteintech (Wuhan, China)), anti−p65 (1:1000, 10745−1−AP, Proteintech), anti−TRAF6 (1:5000, 66498−1−Ig, Proteintech), anti−IRAK1 (1:1000, 10478−2−AP, Proteintech), and anti−p−p65 (1:500, AP3749a, Abcepta (Suzhou, China)). Among the primary antibodies used in Western blotting, only anti-TRAF6 (clone number: 1D1E1) was a murine monoclonal antibody, while the remaining were rabbit polyclonal antibodies.

Mao J., Li S., Fu R., Wang Y., Meng J., Jin Y., Wu T, & Zhang M. (2023). Sea Cucumber Hydrolysate Alleviates Immunosuppression and Gut Microbiota Imbalance Induced by Cyclophosphamide in Balb/c Mice through the NF-κB Pathway. Foods, 12(8), 1604.

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Histological and Enzymatic Liver Assessment

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According to standard protocols, liver sections were stained with hematoxylin and eosin solutions and mounted.²³ Then placed under a light microscope to observe histological changes. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) kits (Nanjing Jiancheng Biotechnology Research Institute) were used to quantify serum ALT and AST levels according to the manufacturer's instructions. The amounts of ALT and AST were determined according to a standard curve.

Chen D., Zeng R., Teng G., Cai C., Pan T., Tu H., Lin H., Du Q., Wang H, & Chen Y. (2021). Menstrual blood‐derived mesenchymal stem cells attenuate inflammation and improve the mortality of acute liver failure combining with A2AR agonist in mice. Journal of Gastroenterology and Hepatology, 36(9), 2619-2627.

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Protocols for Metabolic Biomarker Analysis

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PSS was extracted by using the methods presented in the literature [33 (link)]. Total triglyceride (TG), total cholesterol (TCHO), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) kits were purchased from the Nanjing Jiancheng Bioengineering Research Institute (Nanjing, China). ELISA kits for insulin, serum lipopolysaccharide (LPS), and glycosylated hemoglobin (HbA1c) levels were purchased from Shanghai Enzyme Linked Biotechnology Co., Ltd. (Shanghai, China). Blood glucose meters and blood glucose test strips were purchased from Wuding Biotechnology Co., Ltd. (Taiwan, China). STZ was purchased from Sigma Chemical Co. (St. Louis, MO, USA).

Chen Z., Luo J., Jia M., Chai Y, & Bao Y. (2022). Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins. Nutrients, 14(24), 5222.

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