Lithium hydroxide lioh

The compounds methyl benzoate (1), n-propyl benzoate(3), n-butyl benzoate (4), methyl 2-naphthoate (7), phenylacetate (9), ethyl 2-bromobenzoate (10), ethyl 3-bromobenzoate (11), ethyl 4-bromobenzoate (12), methyl 8-fluoro-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate (methyl flumazenil ester, 16), methyl 4-aminobenzoate (methyl benzocaine ester, 14), and methyl [1,1′-biphenyl]-4-carboxylate (17) were synthesised at the Laboratório de Avaliação e Síntese de Substâncias Bioativas (LASSBio®, UFRJ, Brazil) with degree of purity ≥98%. Flumazenil (13) was purchased from Cristalia® – Brazil. Formic Acid (96%), acetonitrile and methanol of HPLC grade were purchased from Tedia®-Brazil. Water used for the HPLC analysis was produced using a water purification system (Master System MS2000, Gehaka, Brazil). Ethylbenzoate (2), phenylbenzoate (5), benzylbenzoate(6), methyl 2-phenylacetate (8), ethyl 4-aminobenzoate (benzocaine, 13), bicinchoninic acid kit for protein determination, ethylenediamine-tetra-acetic-acid (EDTA), monobasic and bibasic potassium phosphate, bis(p-nitrophenyl) phosphate sodium salt, magnesium chloride hexahydrate, β-nicotinamide adenine dinucleotide phosphate (NADP), D-glucose-6-phosphate, glucose-6-phosphate dehydrogenase, tetrahydrofuran (THF) and lithium hydroxide (LiOH) were purchased from Sigma Aldrich (St. Louis, MO, USA).

The water slurry of cellulose nanofiber (CNF) with a concentration of 5.0 wt% and the degree of polymerization (DP) of 650 was provided by Sugino Machine Limited (Toyama, Japan), and stored in a refrigerator before use. Microcrystalline cellulose (MCC) with a particle size of 20 μm and lithium hydroxide (LiOH, ≥98%) were purchased from Sigma-Aldrich (Tokyo, Japan). Urea and all solvents were supplied from Wako Pure Chemical Corporation (Osaka, Japan). All reagents and solvents were laboratory grade and were used as received.