5 μM purified USP7 core WT or C223A was incubated with 100μM P217564 or DMSO at room temperature for 4 hours, and then the protein/compound reaction products were mixed with equal volumes of 0.1% Trifluoroacetic acid (TFA) before LC-MS injection. The samples were analyzed by reverse-phase liquid chromatography coupled to UV and MS detection (Agilent, 6220 Accurate-Mass Time-of-Flight (TOF) LC/MS). For the conjugation site mapping experiment, trypsin-digested USP7/P217564 covalent complex was analyzed by high-resolution LC-MS/MS.
6220 accurate mass time of flight lc ms
Manufactured by Agilent Technologies
The 6220 Accurate-Mass Time-of-Flight (TOF) LC/MS is a liquid chromatography-mass spectrometry (LC/MS) system designed for high-resolution, accurate-mass analysis. It provides precise mass measurement and identification of compounds in complex samples.
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Lab products found in correlation
2 protocols using 6220 accurate mass time of flight lc ms
1
USP7 Covalent Complex Characterization
2
Synthesis and Characterization of Platinum Complexes
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Preparations
were carried out under atmospheric conditions, except for those that
required silver reagents, which were conducted in the dark under an
N2 atmosphere. Synthesis grade solvents were employed in
all cases. The triazolium iodide salt,67 (link) (Pr4N)2[Pt2Cl6],68 (link) and PhICl269 (link) were synthesized according to reported procedures. All other reagents
were obtained from commercial sources. Elemental analyses were carried
out with a LECO CHNS-932 microanalyzer. Electrospray ionization high-resolution
mass spectra (ESI-HRMS) were recorded on an Agilent 6220 Accurate-Mass
time-of-flight (TOF) LC/MS. NMR spectra were registered on a 600 MHz
Bruker Avance spectrometer at 298 K. The variable-temperature NMR
spectra of1 were registered on a 300 MHz Bruker Avance
spectrometer. Chemical shifts (δ) were referenced to residual
signals of nondeuterated solvent and are given in ppm downfield from
tetramethylsilane. Abbreviations: s, singlet: d, doublet; t, triplet,
q, quartet; p, pentet; sext, sextet; and m, multiplet.
were carried out under atmospheric conditions, except for those that
required silver reagents, which were conducted in the dark under an
N2 atmosphere. Synthesis grade solvents were employed in
all cases. The triazolium iodide salt,67 (link) (Pr4N)2[Pt2Cl6],68 (link) and PhICl269 (link) were synthesized according to reported procedures. All other reagents
were obtained from commercial sources. Elemental analyses were carried
out with a LECO CHNS-932 microanalyzer. Electrospray ionization high-resolution
mass spectra (ESI-HRMS) were recorded on an Agilent 6220 Accurate-Mass
time-of-flight (TOF) LC/MS. NMR spectra were registered on a 600 MHz
Bruker Avance spectrometer at 298 K. The variable-temperature NMR
spectra of
spectrometer. Chemical shifts (δ) were referenced to residual
signals of nondeuterated solvent and are given in ppm downfield from
tetramethylsilane. Abbreviations: s, singlet: d, doublet; t, triplet,
q, quartet; p, pentet; sext, sextet; and m, multiplet.
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