Immunophenotyping of peripheral blood lymphocyte subsets were mainly based on Reference [31 (link)]. Briefly, EDTA-anticoagulated whole blood was split into 2 parts and stained with different panels of antibodies to explore T cells and B cells separately. For T cell subsets, the antibody panel contained CD3-PerCP-Cy5.5, CD4-FITC, CD8-BV510, CD45RA-PE-Cy7, CD27-APC, TCR aβ-PE, and TCR γδ-BV421. For B cell subsets, the antibody panel contained CD19-APC, CD27-V450, IgD-BV510, CD24-PE, and CD38-PerCP-Cy5.5 (All antibodies were purchased from BD Biosciences). After 20 min, erythrocytes in blood sample were lysed by red cell lysis buffer (TIANGEN, China). Then cells were centrifuged and washed twice with PBS. After resuspended, the cells were acquired on BD FACS Canto II, and the data were analyzed using FlowJo (V10.4, BD Biosciences).
Cd27 v450
Manufactured by BD
The CD27-V450 is a lab equipment product that serves a specific core function. It is designed to detect and analyze the presence of CD27, a cell surface receptor, using the V450 fluorescent label. The product provides a tool for researchers to study the expression of CD27 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (1 mentions)
Cd24 pe, by BD (1 mentions)
Cd19 apc, by BD (1 mentions)
Red cell lysis buffer, by Tiangen Biotech (1 mentions)
Cd38 percp cy5, by BD (1 mentions)
Lsr fortessa cytometer, by BD (1 mentions)
Prism 6, by GraphPad (1 mentions)
Cd4 apc, by BD (1 mentions)
Cd3 apc h7, by BD (1 mentions)
Cd8 v500, by BD (1 mentions)
Cd8 v450 rpa t8, by BD (1 mentions)
Cd21 pe, by BD (1 mentions)
Cd5 alexafluor 700, by BD (1 mentions)
Cd38 pe cy7, by BD (1 mentions)
Cd45ra alexa fluor 700, by BD (1 mentions)
2 protocols using cd27 v450
1
Peripheral Blood Lymphocyte Immunophenotyping
2
Multiparametric Flow Cytometry of Immune Cell Subsets
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Healthy donor and patient PBMCs were obtained by using Ficoll density centrifugation and stained for 30 minutes at 48C with the following mouse anti-human antibodies: CXCR5-allophycocyanin (APC; 51505) from R&D Systems (Minneapolis, Minn); CCR7-phycoerythrin (PE)-CF594 (150503), CD27 V450 (M-T271), CD27 Brilliant Violet (M-T271), CD38-PE-Cy7 (HIT2), CD21-PE (B-ly4), CD3-APC-H7 (SK7), CD5-Alexa Fluor 700 (UCHT), CD4-APC (RPA-T4), CD4-Brilliant Violet 605 (RPA-T4), CD45RA-Alexa Fluor 700 (HI100), CD8-V500 (RPA-T8), CD8-fluorescein isothiocyanate (FITC; HIT8a), IgM-APC (G20-1273), IgD-FITC (IA6-2), and CD95-PE-Cy7 (DX2) from BD PharMingen (San Jose, Calif); CD19-peridinin-chlorophyll-protein complex-Cy5.5 (HIB19), CD10-APC (CB-CALLA), and CD31-APC (WM59) from eBioscience (San Diego, Calif); CD14-PE-Cy7 (RMO52), CD56-PE (N901), CD4-PE (13B8.2), T-cell receptor (TCR)-Vb11-FITC (C21), and TCR-Va24-PC-7 (C15) from Beckman Coulter; and CD8-V450 (RPA-T8) from BD Horizon (BD Biosciences, Franklin Lakes, NJ). Stained cells were analyzed on a BD LSR Fortessa cytometer. Data were processed with FlowJo X software (TreeStar, Ashland, Ore) and sketched with Prism 6.0 software (GraphPad Software, La Jolla, Calif).
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