Mda and mpo assay kits

HSS was kindly provided by Professor Sun Xuejun of the Secondary Military Medical University, ROC. Solutions were freshly prepared every week and maintained at 4 °C to ensure a stable hydrogen concentration of 0.6 mM. High-purity OA was purchased from Sigma (St. Louis, MO, USA). MDA and MPO assay kits were obtained from the Nanjing Jiancheng Bioengineering Institute, China. Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α and IL-1β were provided by Peprotech (NJ, USA). Equipment in this study included MK3 microplate reader (Thermo-Scientific, MC, USA), MK2 microplate washer (Thermo-Scientific, MC, USA), electro-heating standing-temperature cultivator (Shanghai Yuejin Medical Instruments Factory, China), TGL-168 centrifuge (Shanghai Anting Scientific Instrument Factory, China), SmartSpec Plus spectrophotometer (Bio-Rad, Hercules, CA, USA), micro-electric tissue homogenizer (Kimble, USA), GM3000 blood gas analyzer (Instrumentation Laboratory, MA, USA), and light microscopy (Olympus, Japan).

To investigate the effect of parecoxib on the oxidative stress reaction, malondialdehyde (MDA) levels in BALF and myeloperoxidase (MPO) activity in lung tissue were detected with MDA and MPO assay kits (Nanjing Jiancheng Corp., Nanjing, Jiangsu, China).