Albumin and urea production as well as lactate dehydrogenase (LDH) release were measured before the drug-metabolism study (4 days postseeding). Albumin production was measured in supernatant using a human albumin enzyme-linked immunosorbent assay (Assay Pro, St. Charles, MO). Urea was quantified with a colorimetric assay kit (BioAssay Systems, Hayward, CA) and LDH secretion was measured using the CytoTox 96 nonradioactive cytotoxicity assay (Promega, Southampton, UK). Albumin, urea, and LDH were also measured postdose at the end of the drug-metabolism study (day 5 for wells treated with phenacetin; day 6 for wells treated with diclofenac, propranolol, lidocaine, and ibuprofen; and day 7 for wells treated with prednisolone). At the end of the experiment, the scaffolds/tissues were removed and washed with phosphate-buffered saline. Bright field images were taken using an inverted light microscope (Leica, Milton Kaynes, UK).
Colorimetric assay kit
Manufactured by BioAssay Systems
Sourced in United States
The Colorimetric assay kit is a laboratory equipment designed to quantify the concentration of a specific analyte in a sample. It utilizes a colorimetric detection method, where the interaction between the analyte and a reagent produces a color change that can be measured using a spectrophotometer or microplate reader. The core function of this kit is to provide a reliable and accurate method for the quantitative determination of the target analyte in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Human albumin enzyme linked immunosorbent assay, by Assaypro (2 mentions)
Cytotox 96 non radioactive cytotoxicity assay, by Promega (2 mentions)
Inverted light microscope, by Leica (1 mentions)
Cell proliferation reagent wst 1, by Roche (1 mentions)
Gsh glo glutathione assay, by Promega (1 mentions)
Synergy ht plate reader, by Agilent Technologies (1 mentions)
Spectrafluor, by Tecan (1 mentions)
Colorimetric assay kit, by Cayman Chemical (1 mentions)
11 protocols using colorimetric assay kit
1
Liver Tissue Function Biomarkers
2
Quantifying Acetylcholine in Neural Stem Cells
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The ACh concentration of NSCs after 5 days of culture with/without 3 days of Aβ incubation was measured by using a colorimetric assay kit (Bioassay Systems, Hayward, CA, USA) according to the manufacturer’s instructions. First, lysis buffer was added to the chip and incubated for 15 min at room temperature (RT). Then, 20 µL of sample was mixed with 80 µL of working reagent and was incubated in the dark for 30 min. The optical density (OD) of the samples was recorded at 570 nm. The color intensity at 570 nm was directly proportional to the ACh concentration in the sample and was calculated from the standard curve.
3
Renal Function Biomarker Assessment
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Serum creatinine was measured using a colorimetric micro-plate assay based on the Jaffe reaction (Bio-Assay Systems, Hayward, CA, USA). A colorimetric assay kit was used to quantify the levels of blood urea nitrogen (BUN) according to the manufacturer’s protocol (Bio-Assay Systems). These measurements, as well as enzyme-linked immunosorbent assay (ELISA) analysis and tissue collagen assays, were performed by several independent laboratory workers blinded to the origin of the samples.
4
Measuring Serum ALT Levels in Mice
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The blood obtained from heart of mice was incubated at room temperature for 1 h, and then centrifuged at 3,000 rpm for 10 min. The resulting supernatants were collected in new tube and measured by using a colorimetric assay kit (BioAssay Systems) to qunatifiy serum alanine aminotransferase (ALT) level.
5
Comprehensive Assay Profile for Liver Microtissues
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Albumin secretion was measured in culture supernatant using a human albumin enzyme-linked immunosorbent assay (Assay Pro, United States). Urea was quantified with a colorimetric assay kit (BioAssay Systems, United States) and lactate dehydrogenase (LDH) activity was measured using the CytoTox 96® Non-radioactive cytotoxicity assay (Promega, United Kingdom). Aspartate transaminase (AST) and alanine aminotransferase (ALT) clinical chemistry assays were performed at the College of American Pathologists certified clinical laboratories in the University of Pittsburgh Medical Center (Pittsburgh, United States). Total glutathione was quantified using the GSH-Glo™ Glutathione Assay (Promega, United Kingdom). Microtissues were lysed from scaffolds in glutathione lysis buffer and each lysate was diluted 100-fold in fresh lysis buffer before luminescent quantification of total glutathione against a standard curve according to vendor instructions. The glutathione content was normalised to total cellular protein. Mitochondrial activity was assessed using Cell Proliferation Reagent WST-1 (Roche, United Kingdom) at a 20-fold dilution in cell culture medium.
6
Serum BUN and Creatinine Determination
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At the end of the experiment, blood was collected from the retro-orbital plexus under anesthesia with 2.5% tribromoethanol (avertin). Serum was obtained by centrifugation at 10,000 relative centrifuge force (RCF) for 30 min at room temperature. Serum BUN and Cr. was determined with a colorimetric assay kit (BioAssay Systems, Hayward, CA) and analyzed with a Biotek Synergy HT plate reader.
7
Enzyme Activity and Hemoglobin Quantification
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Creatine kinase was assessed using a colorimetric assay kit (BioAssay Systems, Hayward, CA, USA) sensitive to 5 U•L -1 . Concentration was calculated based on the absorbance and calibrator read at 340 nm using a plate reader (SpectraFluor, Tecan, Mannedorf, Switzerland). Hemoglobin concentrations were measured using a colorimetric assay kit (Cayman Chemical) sensitive to 0.005 g•dl -1 . Concentration was calculated from standard curve. Values were adjusted for percentage changes in plasma volume as previously described ( 14).
8
Creatinine and BUN Quantification
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Serum creatinine was measured using colorimetric microplate assay based on the Jaffe reaction (BioAssay Systems, Hayward, CA, USA). A colorimetric assay kit was used to quantify the levels of blood urea nitrogen (BUN) in accordance with the supplier's protocol (BioAssay Systems).
9
Measurement of Plasma and Urine Biomarkers
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Plasma and urine levels of creatinine (Cr) and N-acetyl-β-d -glucosaminidase (NAG) were measured using commercially available colorimetric assay kits purchased from BioAssay Systems (Hayward, CA) and Lengton Bioscience Co. (Shanghai, China), respectively.
10
Colorimetric Assay for Pyruvate and Lactate
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Intracellular pyruvate and lactate were measured by colorimetric assay kits (BioAssay Systems, CA, USA) according to manufacturer’s instruction. Briefly, 10 µl of samples or standards were transferred into 96-well plates in duplicates. Ninety microliters of working solution were added into each wells and incubated for 30 min at room temperature. Optical density at 570 nm was read and concentration of pyruvate or lactate was calculated from standard curve.
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