Rat anti mouse b220 mab

Manufactured by BD

Rat anti-mouse B220 mAb is a monoclonal antibody that binds specifically to the B220 protein expressed on the surface of mouse B cells. This antibody is commonly used in flow cytometry and other immunological techniques to identify and quantify B cell populations.

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Lab products found in correlation

Alexa fluor 594 goat anti rat, by Thermo Fisher Scientific (2 mentions) Rat anti mouse fitc conjugated gl7 mab, by BD (2 mentions) Rabbit anti mouse cd3 polyclonal ab, by Abcam (2 mentions) Goat anti rabbit cy5, by Thermo Fisher Scientific (2 mentions) Tcs sp5 2 fluorescence microscope, by Leica (2 mentions) Prolong gold antifade reagent with 4 6 diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (2 mentions)

2 protocols using rat anti mouse b220 mab

Immunofluorescence Analysis of Lymphoid Cells

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CLN from PBS-perfused mice were snap-frozen in Tissue-Tek OCT compound (Sakura Finetex, Torrance, CA) and sectioned at 10 μm using a Thermo Shandon cryostat. Sections were fixed with ice cold 70% acetone for 5 min, blocked with 5% bovine serum albumin and 10% goat serum for 1 h and then stained with rat anti-mouse FITC conjugated GL7 mAb (BD Biosciences), rat anti-mouse B220 mAb (BD biosciences) and rabbit anti-mouse CD3 polyclonal Ab (Abcam, Cambridge, MA) overnight at 4 °C. Samples were then incubated with secondary Ab using Alexa Fluor 594 goat anti-rat (Life Technologies, Grand Island, NY) and Cy5 goat anti-rabbit (Life Technologies, Grand Island, NY) Ab for 1 h at room temperature. Sections were mounted with ProLong Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Grand Island, NY) and examined using a Leica TCS SP5 II fluorescence microscope (Lawrenceville, GA).

Phares T.W., DiSano K.D., Stohlman S.A., Segal B.M, & Bergmann C.C. (2016). CXCL13 promotes isotype-switched B cell accumulation to the central nervous system during viral encephalomyelitis. Brain, Behavior, and Immunity, 54, 128-139.

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Cryosectioning and Immunofluorescent Staining

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CLN from PBS-perfused mice were snap-frozen in Tissue-Tek OCT compound (Sakura Finetex, Torrance, CA) and sectioned at 10 μm using a Thermo Shandon cryostat. Sections were fixed with ice cold 70% acetone for 5 min, blocked with 5% bovine serum albumin and 10% goat serum for 1 hr and then stained with rat anti-mouse FITC conjugated GL7 mAb (BD Biosciences), rat anti-mouse B220 mAb (BD biosciences) and rabbit anti-mouse CD3 polyclonal Ab (Abcam, Cambridge, MA) overnight at 4°C. Samples were then incubated with secondary Ab using Alexa Fluor 594 goat anti-rat (Life Technologies, Grand Island, NY) and Cy5 goat anti-rabbit (Life Technologies, Grand Island, NY) Ab for 1 hr at room temperature. Sections were mounted with ProLong Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Grand Island, NY) and examined using a Leica TCS SP5 II fluorescence microscope (Lawrenceville, GA).

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