Rat 8 ohdg elisa kit

Seven days after surgery, rat peritendinous tissues were homogenized in cold 1 × PBS, followed by two freeze–thaw cycles. Then, the homogenates were centrifuged at 5000 × g, 4 °C for 5 min. The supernatants were aliquotted and assayed immediately. The tissue content of MDA was measured using a Lipid Peroxidation MDA Assay Kit (S0131S, Beyotime Institute of Biotechnology, China) and following manufacturer's instructions. The concentration of nucleic acid oxidative product 8-OHdG was determined using a Rat 8-OHdG ELISA Kit (CSB-E10526r, Cusabio, USA) following manufacturer's instructions. The protein oxidative product 3-NT level was assayed using a rat 3-NT ELISA Kit (F16301, Westang, Shanghai, China).

Mice spleens were dissected at 7 days after radiation exposure and assessed for various oxidative stress parameters. Spleens were homogenized in ice-cold potassium phosphate buffer solution (0.1 mol/l, pH 7.4) using a Potter-Elvehjem homogenizer to give a 10% W/V homogenate. Spleen tissue homogenates were prepared and used to obtain glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (TAC) level estimations. GSH content was determined as previously described by Beutler et al. [21 (link)], SOD activity as in Minami and Yoshikawa [22 (link)] and CAT activity according to Luck [23 ]. TAC level was measured using Randox total antioxidant status kit (UK) according to Rice-Evans and Miller [24 (link)]. LPx product malondialdehyde (MDA) was measured as described by Yoshioka et al. [25 (link)]. Nitric oxide (NO) and protein carbonyl (PCO) in spleen homogenates were estimated according to Miranda et al. [26 (link)] and Levine et al. [27 (link)], respectively. Reactive oxygen species (ROS) was assessed by Rat ROS ELISA Kit (Catalogue Number SL1189Ra, Sun Long Biotech Co., LTD, China). 8-hydroxydeoxyguanosine (8-OHdG), the DNA adduct for oxidative damage, was estimated using Rat 8-OHdG ELISA Kit (Catalog Number CSB-E10526r, Cusabio Technology, LLC, USA).