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Tcrbeta pecy7

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The TCRbeta-PECy7 is a flow cytometry reagent that specifically binds to the beta chain of the T cell receptor (TCR) complex. It can be used to identify and quantify T cells in a sample.

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Lab products found in correlation

Cd3 fitc, by Cytek Biosciences (3 mentions) Cd8 bv711, by BioLegend (3 mentions) Cd4 bv650, by BioLegend (3 mentions) Uea1 fitc, by Vector Laboratories (3 mentions) Cd25 percp cy5, by BioLegend (3 mentions) Lsrfortessa x50, by BD (3 mentions) Cd90 bv785, by BioLegend (3 mentions) Mhc 2 pac blue, by BioLegend (3 mentions) Epcam percpe710, by Thermo Fisher Scientific (3 mentions) Ly51 pe, by Thermo Fisher Scientific (3 mentions) Cd44 alexa fluor rtm700, by BioLegend (3 mentions) Cd62l apc cy7, by BioLegend (3 mentions) Facsdiva, by BD (3 mentions) Aria 2, by BD (3 mentions) Cd45 buv395, by BD (3 mentions) Cd31 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd103 percp cy5, by BioLegend (2 mentions) Cd140a apc, by BioLegend (2 mentions) Annexin 5 binding buffer, by BioLegend (2 mentions) Annexin 5, by BioLegend (2 mentions)

3 protocols using tcrbeta pecy7

1

Multiparameter Flow Cytometry of Immune Cells

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Cells were stained with the following antibodies for analysis CD3-FITC (35–0031, Tonbo Bioscience), CD8-BV711 (100748, BioLegend), CD4-BV650 (100546, BioLegend), CD45-BUV395 (565967, BD Biosciences), CD90-BV785 (105331, BioLegend), CD11c-APC (20–0114, Tonbo Biosciences), MHC-II-Pac Blue (107620, BioLegend), CD103-PercPCy5.5 (121416, BioLegend), CD11b-A700 (557960, BD PharMingen), EpCAM-PercPe710 (46–5791-82, eBioscience), Ly51-PE (12–5891-83, eBioscience), CD31-PECy7 (25–0311-82, eBioscience), CD140a-APC (135907, BioLegend), UEA1-FITC (FL-1061, Vector Laboratories), TCRbeta-PECy7 (109222, BioLegend), CD62L-APC-Cy7 (104427, BioLegend), CD44-Alexa Fluor RTM700 (56–0441-82, BioLegend), CD25-PercP-Cy5.5 (102030, BioLegend). Annexin V staining (640906, BioLegend) was performed in Annexin V binding buffer (422201, BioLegend). Flow cytometric analysis was performed on an LSRFortessa X50 (BD Biosciences) and cells were sorted on an Aria II (BD Biosciences) using FACSDiva (BD Biosciences) or FlowJo (Treestar Software).
Kinsella S., Evandy C.A., Cooper K., Iovino L., deRoos P.C., Hopwo K.S., Granadier D.W., Smith C.W., Rafii S, & Dudakov J.A. (2021). Attenuation of apoptotic cell detection triggers thymic regeneration after damage. Cell reports, 37(1), 109789.
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2

Multiparameter Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with the following antibodies for analysis CD3-FITC (35–0031, Tonbo Bioscience), CD8-BV711 (100748, BioLegend), CD4-BV650 (100546, BioLegend), CD45-BUV395 (565967, BD Biosciences), CD90-BV785 (105331, BioLegend), CD11c-APC (20–0114, Tonbo Biosciences), MHC-II-Pac Blue (107620, BioLegend), CD103-PercPCy5.5 (121416, BioLegend), CD11b-A700 (557960, BD PharMingen), EpCAM-PercPe710 (46–5791-82, eBioscience), Ly51-PE (12–5891-83, eBioscience), CD31-PECy7 (25–0311-82, eBioscience), CD140a-APC (135907, BioLegend), UEA1-FITC (FL-1061, Vector Laboratories), TCRbeta-PECy7 (109222, BioLegend), CD62L-APC-Cy7 (104427, BioLegend), CD44-Alexa Fluor RTM700 (56–0441-82, BioLegend), CD25-PercP-Cy5.5 (102030, BioLegend). Annexin V staining (640906, BioLegend) was performed in Annexin V binding buffer (422201, BioLegend). Flow cytometric analysis was performed on an LSRFortessa X50 (BD Biosciences) and cells were sorted on an Aria II (BD Biosciences) using FACSDiva (BD Biosciences) or FlowJo (Treestar Software).
Kinsella S., Evandy C.A., Cooper K., Iovino L., deRoos P.C., Hopwo K.S., Granadier D.W., Smith C.W., Rafii S, & Dudakov J.A. (2021). Attenuation of apoptotic cell detection triggers thymic regeneration after damage. Cell reports, 37(1), 109789.
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3

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with the following antibodies for analysis CD3-FITC (35–0031, Tonbo Bioscience), CD8-BV711 (100748, BioLegend), CD4-BV650 (100546, BioLegend), CD45-BUV395 (565967, BD Biosciences), CD90-BV785 (105331, BioLegend), MHC-II-Pac Blue (107620, BioLegend), EpCAM-PercPe710 (46-5791-82, eBioscience), Ly51-PE (12-5891-83, eBioscience), UEA1-FITC (FL-1061, Vector Laboratories), TCRbeta-PECy7 (109222, BioLegend), CD62L-APC-Cy7 (104427, BioLegend), CD44-Alexa Fluor RTM700 (56-0441-82, BioLegend), CD25-PercP-Cy5.5 (102030, BioLegend). Flow cytometry analysis was performed on an LSRFortessa X50 (BD Biosciences) and cells were sorted on an Aria II (BD Biosciences) using FACSDiva (BD Biosciences) or FlowJo (Treestar Software).
Kinsella S., Evandy C.A., Cooper K., Cardinale A., Iovino L., deRoos P., Hopwo K.S., Smith C.W., Granadier D., Sullivan L.B., Velardi E, & Dudakov J.A. (2023). Damage-induced pyroptosis drives endog thymic regeneration via induction of Foxn1 by purinergic receptor activation. bioRxiv.
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