MSU were purchased from the Jiancheng Institute of Biotechnology (Nanjing, China). Pentobarbital sodium, paraformaldehyde and physiologic saline solution were purchased from Shanghai Chemical Reagent Co. (Shanghai, China). The darutoside with more than 97.4% purity (batch number: BCBS7438) were obtained from Chengdu Cloma Biological Co., Ltd. (China) and the HPLC chromatography was shown in Supplementary Figure S1 . Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-8 (IL-8), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) assay were purchased from Assay R&D Systems. Nuclear factor kappa-B (NF-κB) and interleukin-10 (IL-10) were purchased from Biowest (Nuaillé, France) and HyClone (Logan, Utah, United States), respectively. Blood uric acid (UA) was bought from Chondex (Redmond, WA, United States). LC-MS grade acetonitrile (ACN) and methanol (MeOH) were purchased from Merck (Darmstadt, Germany), and formic acid (FA) was purchased from Sigma Chemicals Ltd. (St. Louis, MO, United States). Double-distilled water was purified using a Millipore water purification system (Millipore, Bedford, MA). Leucine enkephalin as a standard substance with the purity more than 99.2% were obtained from Hyclone (Logan, UT, United States). Other reagents used were of analytical grade and were purchased locally.
Lc ms grade acetonitrile acn
Manufactured by Merck Group
Sourced in Germany
LC–MS grade acetonitrile (ACN) is a high-purity solvent used in liquid chromatography-mass spectrometry (LC-MS) applications. It is a colorless, volatile liquid with a low viscosity and high polarity. LC-MS grade ACN meets stringent quality standards to ensure consistent performance and minimal interference in sensitive analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium acetate nh4oac, by Merck Group (4 mentions)
Ammonium hydroxide nh4oh, by Merck Group (4 mentions)
Water h2o, by Honeywell (3 mentions)
Formic acid, by Merck Group (3 mentions)
Methanol, by Merck Group (2 mentions)
Lc ms grade methanol meoh, by Honeywell (2 mentions)
Guanidine 8 m solution, by Thermo Fisher Scientific (2 mentions)
Iodoacetamide iam, by Merck Group (2 mentions)
Amicon ultracel 3k centrifugal filters, by Merck Group (2 mentions)
Urea, by Merck Group (2 mentions)
Milli q system, by Merck Group (2 mentions)
Ammonium formate, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Paraformaldehyde (pfa), by Shanghai Chemical Reagent (1 mentions)
Sodium pentobarbital, by Shanghai Chemical Reagent (1 mentions)
Millipore water purification system, by Merck Group (1 mentions)
Methanol meoh, by Merck Group (1 mentions)
Dithioerythritol dte, by Merck Group (1 mentions)
Ldqwlc cam ek, by Thermo Fisher Scientific (1 mentions)
18 protocols using lc ms grade acetonitrile acn
1
Darutoside Modulates Inflammatory Responses
2
Quantitative Bovine Milk Proteome Analysis
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α-LA standard (≥85% purity) from bovine milk, modified porcine trypsin (proteomics grade), iodoacetamide, dithioerythritol (DTE), and triethylammonium bicarbonate (TEAB) buffer (1M) were obtained from Sigma-Aldrich Inc. (Steinheim, Germany). LC-MS grade acetonitrile (ACN) and formic acid (FA) were obtained from Merck (Damstadt, Germany) and Honeywell Fluka (Roskilde, Denmark), respectively. β-CN standard (>90% purity) was purified from bovine milk according to Petrat-Melin et al. (2015) (link). The purity relative to total protein was determined by LC/ESI-MS. The peptide standards (LDQWLC[CAM]EK, VGINYWLAHK, VLPVPQK and AVPYPQR) and isotope labelled peptides as internal standards (ISTD) (LDQWLC[CAM]EK∗, VGINYWLAHK∗, VLPVPQK∗ and AVPYPQR∗ with the asterisk representing isotope labelled C13 and N15 at K or R) were purchased from Thermo Fisher Scientific (Biopolymers, Ulm, Germany) as AQUA Ultimate. Purity of these peptides was above 97% as in the certificates.
3
Simultaneous Quantification of Tramadol Metabolites
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Tramadol (CAS:27203-92-5), O-desmethyltramadol (CAS:144830-15-9), apatinib (CAS: 811803-05-1) and midazolam (CAS:59467-70-8) (used as internal standard, IS) were bought from Shanghai Canspec Scientific & Technology Co., Ltd. LC–MS grade acetonitrile (ACN) and methanol were purchased from Merck (Darmstadt, Germany). Formic acid of HPLC grade (FA, purity 99.9%) was obtained from J&K scientific Ltd. (Shanghai, China). The reduced nicotinamide adenine dinucleotide phosphate (NADPH, coenzymes for in vitro incubation systems) was purchased from Roche Pharmaceutical Ltd. (Basel, Switzerland). Carboxymethylcellulose sodium salt (CMC-Na) was from Sigma-Aldrich Company (Shanghai, China). Pooled RLM and HLM (enzymes for simulating metabolism in vitro) were bought from Corning Life Sciences Co., Ltd. Recombinant human CYP2D6.1 and cytochrome b5 (coenzymes for in vitro incubation systems) were kind gifts from Beijing Hospital (Beijing, China).
4
Amino Acid Quantification by LC-MS
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A multi-standard solution of amino acids was purchased from Wako Pure Chemistry (Wako Chemical Co., Japan). This multi-standard solution contained l -arginine (Arg), l -aspartic acid (Asp), l -cysteine (Cys), l -glutamic acid (Glu), l -isoleucine (ILe), l -leucine (Leu), dl -lysine (Lys), l -methionine (Met), d -phenylalanine (Phe), dl -proline (Pro), l -serine (Ser), l -threonine (Thr), and dl -valine (Val). The standard solution was stored in darkness at 4 °C. Working standard solutions were prepared daily by diluting the mixed standard stock solution in appropriate proportions. l -Valine-2,3,4,4,4,4′,4′,4-d8, 98 atom% D and l -cysteine-2,3,3-d3, 98 atom% D were purchased from Aldrich, Merck (Darmstadt, Germany). A working solution containing 1 μmol mL−1 of each amino acid was prepared in 0.1 M formic acid (FA). All the above solutions were stored at −20 °C until use.
LC-MS grade acetonitrile (ACN) and ammonium formate, ammonium acetate, ammonia, acetic acid and FA from Merck (Darmstadt, Germany) were used to prepare the chromatographic mobile phases. Deionized water from a Millipore Milli-Q water purification system was used to prepare all aqueous solutions. 36.5% (v/v) hydrochloric acid employed for sample preparation was purchased from Merck (Darmstadt, Germany).
LC-MS grade acetonitrile (ACN) and ammonium formate, ammonium acetate, ammonia, acetic acid and FA from Merck (Darmstadt, Germany) were used to prepare the chromatographic mobile phases. Deionized water from a Millipore Milli-Q water purification system was used to prepare all aqueous solutions. 36.5% (v/v) hydrochloric acid employed for sample preparation was purchased from Merck (Darmstadt, Germany).
5
Liver S9 Fraction Characterization
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Pooled human liver S9 fraction (H0610.S9) and NADPH regenerating system (K5100-5) were purchased from Sekisui Xenotech (Kansas City, KS, USA). The cofactors adenosine 3′-phosphate 5′-phosphosulfate lithium salt hydrate (PAPS), acetyl-coenzyme A (acetyl-CoA), uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The glutathione (GSH) was purchased from J&K (Shanghai, China). The NIST urine (SRM 3667) and NIST plasma (SRM 1950) samples were purchased from Ango Biotechnology Co. (Shanghai, China). LC–MS grade methanol (MeOH) and water (H2O) were purchased from Honeywell (Muskegon, MI, USA). LC–MS grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). Ammonium hydroxide (NH4OH) and ammonium acetate (NH4OAc) were purchased from Sigma (St. Louis, MO, USA). Other chemical standards were purchased from Sigma-Aldrich (St. Louis, MO), J&K (Shanghai, China), and TopScience (Shanghai, China).
6
LC-MS Analysis of Metabolites
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LC–MS grade methanol (MeOH) and water (H2O) were purchased from Honeywell (Muskegon, MI, USA). LC–MS grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). LC–MS grade methylene dichloride (CH2Cl2) was purchased from Fisher Scientific (Morris Plains, NJ, USA). Ammonium hydroxide (NH4OH) and ammonium acetate (NH4OAc) were purchased from Sigma (St. Louis, MO, USA). The chemical standard succinoadenosine was purchased from J&K (Shanghai, China), while other chemical standards were purchased from TopScience (Shanghai, China).
7
Quantitative Analysis of BPA and BPA-d16
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BPA (≥99 %) and BPA-d16 (98 % D) were purchased from Sigma-Aldrich (Deisenhofen, Germany), and LC-MS-grade acetonitrile (ACN) was purchased from Merck KGaA (Darmstadt, Germany). Ultra-pure water was obtained with the help of an HLP 5 system from Hydrolab (Wiślina, Poland).
8
Solvent Preparation for Analytical Procedures
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LC-MS-grade acetonitrile (ACN) and HPLC-grade methanol (MeOH) were purchased from Merck. Distilled water was used throughout all procedures.
9
Characterization of Biosimilar Monoclonal Antibodies
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The biosimilar of Cetuximab (IgG1 type) used in this paper was expressed in transfected CHO cells lines by our lab. The biosimilar of bevacizumab was given by Wuhan Xinshengye Co. Limited (Wuhan, China), which was also expressed by CHO cell lines. Peptide-N-glycosidase F (PNGase F) and endoglycosidase buffer kit were obtained from LCP Biomed (Lianyungang, China). Dimethyl sulfoxide (DMSO), 2-aminobenzoic acid (2-AA), 2-Picoline-borane (2-PB), trifluoroacetic acid (TFA), porous graphitic carbon (PGC) cartridges, microcrystalline cellulose (MCC) and ammonium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A). Glacial acetic acid, ethanol, 1-butanol were obtained from Aladdin (Shanghai, China). LC-MS grade Formic acid (FA), pure water for NanoLC ESI-MS and PierceTM Fab Preparation Kit were attained from Thermo Fisher Scientific (Waltham, MA, U.S.A). Cetuximab, LC-MS grade Acetonitrile (ACN) and methanol were purchased from Merck KGaA (Darmstadt, Germany). All reagents were HPLC grade and all the reaction solutions were prepared with water purified by the Direct-Q system (Millipore, Bedford, MA).
10
Netupitant Characterization in Aqueous Media
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Netupitant was purchased from Shanghai Seebio Biotech, Inc. (Shanghai, China). Ammonium formate (≥99.0%, for HPLC), ammonia solution (25%), and formic acid (99%) were obtained from Sigma-Aldrich (Steinheim, Germany) and Th. Geyer GmbH & Co. KG (Renningen, Germany). LC/MS-grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). Working solutions of netupitant (10 μM) were prepared by diluting a 1 mM stock solution (prepared in ACN) with 10 mM Ammonium formate buffer. The working solutions were adjusted to three different pH values by combining them respectively with 10 mM Ammonium formate buffers of pH 3.0, 7.4, and 9.0 that had been mixed with ACN at a 50/50 (V/V) ratio. Double-distilled water generated with an Aquatron A4000D water purification system (Cole-Parmer, Staffordshire, UK) was used for all sample preparations.
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