The assay of cell proliferation was performed with Cell-Light Apollo567 In Vitro Kit (RiboBio, Guangzhou, China). Complete medium was used to re-suspend the Schwann cells that were then tallied and plated on 96-well poly-L-lysine-coated plates. EdU was applied and the cells were cultured after cell transfection. The cells were fixed with phosphate buffered saline containing 4% formaldehyde and stained with Apollo 567 (RiboBio, Guangzhou, China) and Hoechst 33342 (RiboBio). Schwann cell proliferation analysis was performed using randomly selected images through a fluorescence microscope (Leica, Mannheim, Germany). The proliferating cell numbers were calculated. The average number of proliferating cells in the control group was set as 100%. The cell proliferation rate of p-GV230-Claudin-15 group was obtained by dividing by the average number of proliferating cells in the negative control or pGV230 group. The results were presented as fold change.
Cell light apollo567 in vitro kit
Manufactured by RiboBio
Sourced in China
The Cell-Light Apollo567 In Vitro Kit is a laboratory equipment product designed for cell-based experiments. The kit provides the necessary components for conducting in vitro studies.
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Lab products found in correlation
3 protocols using cell light apollo567 in vitro kit
1
Schwann Cell Proliferation Assay
2
Cell Proliferation Assay with Apollo567
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A Cell-Light Apollo567 In Vitro Kit (RiboBio, Guangzhou, China) was used to assess the cell proliferation ability. Specific steps are described in detail in a previous study7 (link).
3
Quantifying Ovarian Cancer Cell Proliferation
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The cell proliferation was detected using Cell-Light Apollo567 in vitro kit (RiboBio, Guangzhou, China). Ovarian cancer cells were cultured in 1640 medium. EdU was applied at 20 μM. The cells were fixed with 4% paraformaldehyde and stained with Apollo 567 and Hoechst 33342 [12 (link)].
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