Log In Sign up
The largest database of trusted experimental protocols

M60 009rdpd

Manufactured by Bio-Rad
Visit Supplier
Sourced in United States

The M60-009RDPD is a laboratory equipment product manufactured by Bio-Rad. It serves as a core function device for various laboratory applications. No further details on the intended use or features of this product can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Bio plex 200 system, by Bio-Rad (4 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Dy453 05, by R&D Systems (1 mentions) Luminex 200 instrument system, by DiaSorin (1 mentions) Bio plex manager software 6, by Bio-Rad (1 mentions) Bio plex 200, by Bio-Rad (1 mentions) Varioskan lux multimode microplate reader, by Thermo Fisher Scientific (1 mentions) Pro mouse cytokine 23 plex, by Bio-Rad (1 mentions) Il 18 elisa kit, by Thermo Fisher Scientific (1 mentions) Durapore membrane, by Merck Group (1 mentions) Bio plex pro mouse cytokine 23 plex assay, by Bio-Rad (1 mentions) Bio plex manager software version 4, by Bio-Rad (1 mentions) Luminex 200 labmap system, by DiaSorin (1 mentions) Ultra sensitive mouse insulin elisa kit 90080, by Crystal Chem (1 mentions) Cfx96 real time instrument, by Bio-Rad (1 mentions) Sybr green, by Bio-Rad (1 mentions) Sandwich elisa kit, by R&D Systems (1 mentions) Ripa lysis buffer, by Thermo Fisher Scientific (1 mentions)

21 protocols using m60 009rdpd

1

Kidney Injury Biomarker and Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For kidney injury biomarker and cytokine profiling shown in Figures 2B and S2, supernatants were thawed on ice, diluted 1:2 and 1:10 in sample dilution buffer (M60-009RDPD, BioRad), and analyzed by multiplex ELISA using Human Kidney Tox Panel I (Bio-plex,171-ATR1CK, analytes: calbindin, clusterin, GST-π, IL-18, KIM-1, MCP-1), Human Kidney Tox Panel II (Bio-plex,171-ATR2CK, analytes: albumin, beta2 microglobulin, cystatin C, NGAL, OPN, TFF3), and Human Cytokine and Chemokine Panel (Milliplex, HCYTOMAG-60K-PX30, analytes: EGF, G-CSF, GM-CSF, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 [p40], IL-12 [p70], IL-13, IL-15, IL-17, IP-10, MCP-1, MIP-1α, MIP-1β, TNF-α, TNF-β, VEGF, RANTES, and eotaxin/CCL11) followed by analysis using the Bio-Plex 200 Systems (BioRad) according to manufacturer’s instructions. For analysis of human EGF and KIM-1 from Figures 2D, 3, 4, 5, 6, and 7, supernatants were thawed on ice, diluted 1:2 and 1:10 in sample dilution buffer (BioRad, M60-009RDPD), and analyzed by multiplex ELISA using Human Kidney Injury Panel 4 (Millipore, HKI4MAG-99K). Mean concentrations and SDs of triplicate wells were calculated and normalized on vehicle control.
Moisan A., Gubler M., Zhang J.D., Tessier Y., Dumong Erichsen K., Sewing S., Gérard R., Avignon B., Huber S., Benmansour F., Chen X., Villaseñor R., Braendli-Baiocco A., Festag M., Maunz A., Singer T., Schuler F, & Roth A.B. (2016). Inhibition of EGF Uptake by Nephrotoxic Antisense Drugs In Vitro and Implications for Preclinical Safety Profiling. Molecular Therapy. Nucleic Acids, 6, 89-105.
+ Open protocol
+ Expand
2

Multiplex Evaluation of Cytokine Profiles

Check if the same lab product or an alternative is used in the 5 most similar protocols
In the culture supernatants and liver homogenates, the following 27 cytokines and chemokines were measured using a multiplex ELISA kit (M60009RDPD, Bio-Rad). The cytokines and chemokines analysed were IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, basic FGF, Eotaxin, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1 (MCAF), MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF and VEGF. In some experiments, IL-22, IL-1α, IL-12 (p70), MIP-1α, IL-17, IFN-γ, IL-1β (88-7422-88, 88-5019-88, 88-7121-88, 88-56013-88, 88-7371-88, 88-7314-88 and 88-7013-88 from eBiosciences), KC, MIP-1β (DY453-05 and DY451 from R&D) and insulin (10124701, Mercodia) levels were measured using individual ELISA kits.
Tripathi D., Venkatasubramanian S., Cheekatla S.S., Paidipally P., Welch E., Tvinnereim A.R, & Vankayalapati R. (2016). A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nature Communications, 7, 13896.
+ Open protocol
+ Expand
3

Cytokine Profiling in Mtb-Infected Lungs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Using a 23-plex mouse Cytokine Panel (M60009RDPD, Biorad) and a Bio-plex (multiplex ELISA-Luminex) reader, cytokines were analysed in Mtb-infected lung homogenates from NCoR1fl/fl and NCoR1MyeKO mice, according to the recommended protocol. Prior to beginning the process of quantifying cytokines in the lung tissue samples, a commercial BCA test was done as recommended by Thermo Scientific to normalise the amounts of total protein in the lung lysate samples [81 (link)–83 ].
Biswas V.K., Sen K., Ahad A., Ghosh A., Verma S., Pati R., Prusty S., Nayak S.P., Podder S., Kumar D., Gupta B, & Raghav S.K. (2023). NCoR1 controls Mycobacterium tuberculosis growth in myeloid cells by regulating the AMPK-mTOR-TFEB axis. PLOS Biology, 21(8), e3002231.
+ Open protocol
+ Expand
4

Serum Cytokine Profiling Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum was collected by centrifugation at 2000 g for 5 min at 4°C, aliquoted, and stored at -70°C until analysis. A multiplex cytokine assay (M60009RDPD Bio-Rad, Hercules, CA, USA) was performed in accordance with the manufacturer's instructions using the Luminex 200 system instrument (Luminex Corporation).
Morowitz M., Di Caro V., Pang D., Cummings J., Firek B., Rogers M.B., Ranganathan S., Clark R.S, & Aneja R.K. (2017). Dietary supplementation with non-fermentable fiber alters the gut microbiota and confers protection in a murine model of sepsis. Critical care medicine, 45(5), e516-e523.
+ Open protocol
+ Expand
5

Multiplex Quantification of Plasma Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma concentrations of hormones (insulin and glucagon) and adipokines (leptin, resistin, and plasminogen activator inhibitor 1 (PAI-1)) were quantified in triplicate using a multiplex detection kit (171-F7001M, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s protocol. Plasma concentrations of adiponectin and plasma cytokines (interferon γ (IFN-γ), monocyte chemoattractant protein 1 (MCP-1), and tumor necrosis factor α (TNF-α)) were quantified in triplicate using a detection kit (171-F7002M, Bio-Rad, Hercules, CA, USA) and multiplex detection kit (M60-009RDPD, Bio-Rad), respectively, according to the manufacturer’s instructions.
Choi J.Y., Kim Y.J., Ryu R., Cho S.J., Kwon E.Y, & Choi M.S. (2016). Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles. Nutrients, 8(10), 640.
+ Open protocol
+ Expand
6

Cytokine Profiling in LPS, polyI:C, and CpG-Treated Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mice were injected with either LPS (3 mg/kg), polyI:C (5 mg/kg), CpG (100 nmol/kg), or saline, returned to their home cage, and decapitated through cervical dislocation 2 hr after treatment. Trunk blood was obtained by exsanguination, centrifuged at 800 g at 4°C for 15 min, and the resultant serum was frozen immediately and stored at −80°C until use. The cytokine concentrations in the blood were determined using ELISA (catalog BMS606INST, BMS614INST, BMS607/2INST, BMS6002INST, BMS613INST; eBioscience, San Diego, CA, USA) and multiplex assays (catalog M60-009RDPD; Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
AZUMA Y.T, & NISHIYAMA K. (2020). Interleukin-19 enhances cytokine production induced by lipopolysaccharide and inhibits cytokine production induced by polyI:C in BALB/c mice. The Journal of Veterinary Medical Science, 82(7), 891-896.
+ Open protocol
+ Expand
7

Multiplex Cytokine Quantification in BAL

Check if the same lab product or an alternative is used in the 5 most similar protocols
To quantify cytokines in the BAL, 50 μl of cell free undiluted supernatant from each sample was analyzed using mouse cytometric bead array flex sets (M60-009RDPD, Bio-Rad) according to the manufacturer’s instructions. Individual standards were reconstituted and serially diluted in a 96-well plate and all samples assessed by means of specific antibody-coated 7.5-mm capture beads and phycoerythrin-conjugated detection antibodies. Cytokine measurements were performed using the BioPlex-200 (Bio-Rad). Respective standard curves and cytokine concentrations were calculated with the BioPlex Manager Software 6.1 (Bio-Rad).
Hanschmann E.M., Berndt C., Hecker C., Garn H., Bertrams W., Lillig C.H, & Hudemann C. (2020). Glutaredoxin 2 Reduces Asthma-Like Acute Airway Inflammation in Mice. Frontiers in Immunology, 11, 561724.
+ Open protocol
+ Expand
8

Multiplex Cytokine and Tumor Marker Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum levels of mouse cytokines were analyzed by the Luminex Bio-Plex system. Cell culture supernatant cytokines were measured by the Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific). The mouse cytokine 23-multiplex assay was carried out according to the manufacturer’s instructions (Bio-Rad, catalog M60009RDPD). Specific cytokines such as IL23, IL6, IL1β, TGF-β, TNF-α, and IL17A were measured with corresponding cytokine ELISA kits (Thermo Fisher Scientific, catalog 88-7230-88, catalog 88-7064-88, catalog 88-7013-88, catalog 88-8350-88, catalog 88-7324-88, and catalog 88-7371-88). In addition, tumor markers CEA (Lifespan Biosciences Inc, catalog LS-F5042) and CA 19-9 (Lifespan Biosciences Inc, catalog LS-F24309) were used to distinguish between benign and malignant tumors.
Dong X., Qi M., Cai C., Zhu Y., Li Y., Coulter S., Sun F., Liddle C., Uboha N.V., Halberg R., Xu W., Marker P, & Fu T. (2024). Farnesoid X receptor mediates macrophage-intrinsic responses to suppress colitis-induced colon cancer progression. JCI Insight, 9(2), e170428.
+ Open protocol
+ Expand
9

Quantifying Plasma Adipokines and Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma concentrations of insulin and adipokines (leptin, resistin, and plasminogen activator inhibitor 1 (PAI-1)) were quantified using a multiplex detection kit (171-F7001M, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s protocol. Plasma concentrations of adiponectin and plasma cytokines (interferon γ (IFN-γ), and monocyte chemoattractant protein 1 (MCP-1)) were quantified using a detection kit (171-F7002M, Bio-Rad, Hercules, CA, USA) and multiplex detection kit (M60-009RDPD, Bio-Rad), respectively, according to the manufacturer’s instructions.
Choi J.Y., Kim Y.J., Cho S.J., Kwon E.Y., Ryu R, & Choi M.S. (2017). Metabolic Effect of an Oriental Herbal Medicine on Obesity and Its Comorbidities with Transcriptional Responses in Diet-Induced Obese Mice. International Journal of Molecular Sciences, 18(4), 747.
+ Open protocol
+ Expand
10

Multiplex Cytokine Profiling of Frozen Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokines were measured in blood samples frozen at −80°C using a multiplex cytokine assay kit (PRO Mouse Cytokine 23-PLEX, 1×96 [Reference: M60009RDPD/Bio-Rad Laboratories]). Cytokine levels were expressed in picograms per milliliter. The concentrations of cytokines were log transformed in order to limit influence of high concentrations and to normalize distributions.
Audi C., Baïz N., Maesano C.N., Ramousse O., Reboulleau D., Magnan A., Caillaud D, & Annesi-Maesano I. (2017). Serum cytokine levels related to exposure to volatile organic compounds and PM2.5 in dwellings and workplaces in French farmers – a mechanism to explain nonsmoking COPD. International Journal of Chronic Obstructive Pulmonary Disease, 12, 1363-1374.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!