Anti vim

Manufactured by Abcam

Sourced in United Kingdom

Anti-VIM is a monoclonal antibody that detects the Vimentin protein. Vimentin is a type III intermediate filament protein that is expressed in mesenchymal cells. This antibody can be used for the detection of Vimentin by various analytical methods.

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Mouse anti-VIM (3 citations)

5 protocols using anti vim

EMT Regulation and Src/STAT3 Signaling

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The study protocol was performed according to the principles of the Declaration of Helsinki and the ethics committee of the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. Informed consent was obtained from all patients.
The following primary antibodies were purchased from Abcam (Cambridge, England): anti-E-cad, anti-Vim, anti-Snail, anti-N-cad, anti-Twist, anti-PCNA, anti-Bax, anti-Bcl-2, anti-ZEB1, anti-NEDD9, anti-Myb, and anti-GAPDH. The following primary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA): anti-t-SRC, anti-p-SRC, anti-t-STAT3, and anti-p-STAT3. PP1, an SRC inhibitor, was purchased from Selleck Chemicals (Houston, TX, USA).

Xue Y., Wu T., Sheng Y., Zhong Y., Hu B, & Bao C. (2021). MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9. Aging (Albany NY), 13(14), 18924-18945.

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Immunofluorescence Staining of Brain Tissue

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The frozen slice was blocked by 10% goat serum (Sigma, USA) for 1 h at room temperature, then incubated with the primary antibody of anti-GFAP (chicken, Millipore, USA; rabbit, Dako, Japan), anti-VIM (chicken, Abcam, USA), anti-SerpinA3N (goat, R&D Systems), anti-Iba1 (rabbit, FUJIFILM Wako Pure Chemical Corporation, USA), anti-MBP (rabbit, Cell Signaling Technology, USA) at 4 °C overnight, washed three times, then incubated with the fluorescence-labeled second antibody (Invitrogen, USA) 1 h at room temperature; washed three times, and mounted with DAPI. Images were captured with a confocal laser-scanning microscope (Zeiss LSM710, USA) or Fluorescence Microscope BZ-X800 (KEYENCE, Japan).

Qiu S., Palavicini J.P., Wang J., Gonzalez N.S., He S., Dustin E., Zou C., Ding L., Bhattacharjee A., Van Skike C.E., Galvan V., Dupree J.L, & Han X. (2021). Adult-onset CNS myelin sulfatide deficiency is sufficient to cause Alzheimer’s disease-like neuroinflammation and cognitive impairment. Molecular Neurodegeneration, 16, 64.

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Immunofluorescence Analysis of Cell Adhesion Molecules

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Cells (1 × 10⁴/well) were seeded on 8-well chamber slides (Lab-Tech) after transfection. The cells were fixed, permeated and blocked. Then, they were incubated with the following antibodies: anti-VIM (1:200, Abcam), anti-ITGAV (1:1000, Cell Signaling Technology), anti-ITGB1 (1:1000, Cell Signaling Technology), anti-ITGB3 (1:500, Cell Signaling Technology) or anti-CD47 (1:200, Abcam). The secondary antibody, Alexa Fluor 488-conjugated goat anti-mouse IgG or anti-rabbit IgG (1:500, Invitrogen), was applied for 1 hour. Slides were mounted with antifading solution containing 4′-6′-diamidino-2-phenyl-indole (Vector Laboratories). Images were taken using a confocal microscope (Zeiss). All experiments were conducted in triplicate.

Yang S.Y., Choi S.A., Lee J.Y., Park A.K., Wang K.C., Phi J.H., Koh E.J., Park W.Y., Park S.H., Hwang D.W., Jung H.W, & Kim S.K. (2015). miR-192 suppresses leptomeningeal dissemination of medulloblastoma by modulating cell proliferation and anchoring through the regulation of DHFR, integrins, and CD47. Oncotarget, 6(41), 43712-43730.

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Western Blot Analysis of EMT Markers

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Cells were collected and washed with PBS, and protein was extracted using lysis buffer (PMSF: RIPA=1:100, Beyotime Institute of Biotechnology, Haimen, China). Protein concentrations were measured by BCA Protein Assay Reagent (Pierce; Thermo Fisher Scientific, Inc.). Total proteins were separated by 10% SDS-polyacrylamide gel electrophoresis, electro-transferred onto polyvinylidene fluoride membranes (EMD Millipore, Billerica, MA, USA), and blocked with 5% non-fat milk in PBS with 0.05% Tween-20 for 2 h. The membranes were incubated overnight at 4°C with the following antibodies: Anti-STMN1 (cat. no. ab52630; dilution 1:500; Abcam), anti-E-Cad (cat. no. ab40772; dilution 1:500; Abcam), anti-VIM (cat. no. ab92547; dilution 1:500; Abcam), and anti-β-actin (cat. no. ab8227; dilution 1:2,000; Abcam). The membrane was incubated with secondary antibodies for 1 h at room temperature. The immunocomplexes were visualized by enhanced chemiluminescence.

Ke B., Guo X.F., Li N., Wu L.L., Li B., Zhang R.P, & Liang H. (2018). Clinical significance of Stathmin1 expression and epithelial-mesenchymal transition in curatively resected gastric cancer. Molecular and Clinical Oncology, 10(2), 214-222.

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Epithelial-Mesenchymal Transition Pathway

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The study protocol was performed according to the principles of the Declaration of Helsinki and the ethics committee of the A liated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. Informed consent was obtained from all patients.
The following primary antibodies were purchased from Abcam (Cambridge, England): anti-E-cad, anti-Vim, anti-Snail, anti-N-cad, anti-Twist, anti-PCNA, anti-Bax, anti-Bcl-2, anti-ZEB1, anti-NEDD9 and anti-GAPDH. The following primary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA): anti-t-SRC, anti-p-SRC, anti-t-STAT3, anti-p-STAT3. PP1, an inhibitor of SRC, was purchased from Selleck Chemicals (Houston, TX, USA).

Xue Y., Wu T., Sheng Y., Yao Z., Hu B., & Bao C. (2020). MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9 .

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