Rabbit anti loxl2

Paraffin-embedded tissue sections were labeled with mouse SOX9 antibody (Abcam) or its isotype control (Vector Biolabs) detected with anti-mouse IgG conjugated to Alexa 488. To detect LOXL2 expression, the tissues were subjected to rabbit anti-LOXL2 (GeneTex), anti-RFP (Abcam), anti-phospho-SMAD2/3 (Cell Signaling Technology), anti-COL2A1 (Abcam) or its isotype control antibody and detected with anti-rabbit Alexa 488-conjugated antibody or biotin antibody followed by streptavidin-conjugated Texas red, in respective samples. Anti-fade reagent with DAPI was added to all samples. A Zeiss 710 dual scanner confocal microscope with a Plan-Apochromat objective, oil immersion lens, and a CCD detector was used to obtain confocal images. Image acquisition was performed with Zeiss Zen image analysis software (Carl Zeiss Micro Imaging). The image analysis was performed using Zeiss LSM viewer and Image J software (NIH). Z-stack images analysis and 3-dimensional reconstruction was performed by using LOCI and the 3D viewer plug-in of Image J software. Quantification was performed using Image J software as described [15 (link)].

Paraffin-embedded tissue sections were labeled with. To detect LOXL2 expression, the tissues were incubated with rabbit anti-LOXL2 (GeneTex, Inc) or its isotype control antibody and detected with anti-rabbit biotin followed by streptavidin-conjugated Texas red. For immunofluorescence analysis, anti-rabbit IgG conjugated to Alexa 488antibody Anti-fade reagent with DAPI was added to all samples before imaging. Image analysis was performed using Zeiss LSM viewer and Image J software (NIH, USA)^{19 (link),20 (link)}.